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Nogo A (RTN4) (NM_207521) Human Tagged ORF Clone
CAT＃: RC221136
- TrueORF®
RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 5
ORF Plasmid: tGFP
"NM_207521" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221136 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGAAGGAGCAGCCAGGTAACACTATTTCGGCTGGTCAAGAGGATTTCCCATCTGTCCTGCTTG AAACTGCTGCTTCTCTTCCTTCTCTGTCTCCTCTCTCAGCCGCTTCTTTCAAAGAACATGAATACCTTGG TAATTTGTCAACAGTATTACCCACTGAAGGAACACTTCAAGAAAATGTCAGTGAAGCTTCTAAAGAGGTC TCAGAGAAGGCAAAAACTCTACTCATAGATAGAGATTTAACAGAGTTTTCAGAATTAGAATACTCAGAAA TGGGATCATCGTTCAGTGTCTCTCCAAAAGCAGAATCTGCCGTAATAGTAGCAAATCCTAGGGAAGAAAT AATCGTGAAAAATAAAGATGAAGAAGAGAAGTTAGTTAGTAATAACATCCTTCATAATCAACAAGAGTTA CCTACAGCTCTTACTAAATTGGTTAAAGAGGATGAAGTTGTGTCTTCAGAAAAAGCAAAAGACAGTTTTA ATGAAAAGAGAGTTGCAGTGGAAGCTCCTATGAGGGAGGAATATGCAGACTTCAAACCATTTGAGCGAGT ATGGGAAGTGAAAGATAGTAAGGAAGATAGTGATATGTTGGCTGCTGGAGGTAAAATCGAGAGCAACTTG GAAAGTAAAGTGGATAAAAAATGTTTTGCAGATAGCCTTGAGCAAACTAATCACGAAAAAGATAGTGAGA GTAGTAATGATGATACTTCTTTCCCCAGTACGCCAGAAGGTATAAAGGATCGTTCAGGAGCATATATCAC ATGTGCTCCCTTTAACCCAGCAGCAACTGAGAGCATTGCAACAAACATTTTTCCTTTGTTAGGAGATCCT ACTTCAGAAAATAAGACCGATGAAAAAAAAATAGAAGAAAAGAAGGCCCAAATAGTAACAGAGAAGAATA CTAGCACCAAAACATCAAACCCTTTTCTTGTAGCAGCACAGGATTCTGAGACAGATTATGTCACAACAGA TAATTTAACAAAGGTGACTGAGGAAGTCGTGGCAAACATGCCTGAAGGCCTGACTCCAGATTTAGTACAG GAAGCATGTGAAAGTGAATTGAATGAAGTTACTGGTACAAAGATTGCTTATGAAACAAAAATGGACTTGG TTCAAACATCAGAAGTTATGCAAGAGTCACTCTATCCTGCAGCACAGCTTTGCCCATCATTTGAAGAGTC AGAAGCTACTCCTTCACCAGTTTTGCCTGACATTGTTATGGAAGCACCATTGAATTCTGCAGTTCCTAGT GCTGGTGCTTCCGTGATACAGCCCAGCTCATCACCATTAGAAGCTTCTTCAGTTAATTATGAAAGCATAA AACATGAGCCTGAAAACCCCCCACCATATGAAGAGGCCATGAGTGTATCACTAAAAAAAGTATCAGGAAT AAAGGAAGAAATTAAAGAGCCTGAAAATATTAATGCAGCTCTTCAAGAAACAGAAGCTCCTTATATATCT ATTGCATGTGATTTAATTAAAGAAACAAAGCTTTCTGCTGAACCAGCTCCGGATTTCTCTGATTATTCAG AAATGGCAAAAGTTGAACAGCCAGTGCCTGATCATTCTGAGCTAGTTGAAGATTCCTCACCTGATTCTGA ACCAGTTGACTTATTTAGTGATGATTCAATACCTGACGTTCCACAAAAACAAGATGAAACTGTGATGCTT GTGAAAGAAAGTCTCACTGAGACTTCATTTGAGTCAATGATAGAATATGAAAATAAGGAAAAACTCAGTG CTTTGCCACCTGAGGGAGGAAAGCCATATTTGGAATCTTTTAAGCTCAGTTTAGATAACACAAAAGATAC CCTGTTACCTGATGAAGTTTCAACATTGAGCAAAAAGGAGAAAATTCCTTTGCAGATGGAGGAGCTCAGT ACTGCAGTTTATTCAAATGATGACTTATTTATTTCTAAGGAAGCACAGATAAGAGAAACTGAAACGTTTT CAGATTCATCTCCAATTGAAATTATAGATGAGTTCCCTACATTGATCAGTTCTAAAACTGATTCATTTTC TAAATTAGCCAGGGAATATACTGACCTAGAAGTATCCCACAAAAGTGAAATTGCTAATGCCCCGGATGGA GCTGGGTCATTGCCTTGCACAGAATTGCCCCATGACCTTTCTTTGAAGAACATACAACCCAAAGTTGAAG AGAAAATCAGTTTCTCAGATGACTTTTCTAAAAATGGGTCTGCTACATCAAAGGTGCTCTTATTGCCTCC AGATGTTTCTGCTTTGGCCACTCAAGCAGAGATAGAGAGCATAGTTAAACCCAAAGTTCTTGTGAAAGAA GCTGAGAAAAAACTTCCTTCCGATACAGAAAAAGAGGACAGATCACCATCTGCTATATTTTCAGCAGAGC TGAGTAAAACTTCAGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGC CAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTAACAGCCTACATTGCCTTGGCC CTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGTGTGATCCAAGCTATCCAGAAATCAGATGAAG GCCACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAA TTCTGCTCTTGGTCATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTT GATTCTCTGAAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACAC TACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAGGCACAGATAGA TCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAAATCCAAGCAAAAATCCCTGGA TTGAAGCGCAAAGCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221136 protein sequence
Red=Cloning site Green=Tags(s) MDLKEQPGNTISAGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNLSTVLPTEGTLQENVSEASKEV SEKAKTLLIDRDLTEFSELEYSEMGSSFSVSPKAESAVIVANPREEIIVKNKDEEEKLVSNNILHNQQEL PTALTKLVKEDEVVSSEKAKDSFNEKRVAVEAPMREEYADFKPFERVWEVKDSKEDSDMLAAGGKIESNL ESKVDKKCFADSLEQTNHEKDSESSNDDTSFPSTPEGIKDRSGAYITCAPFNPAATESIATNIFPLLGDP TSENKTDEKKIEEKKAQIVTEKNTSTKTSNPFLVAAQDSETDYVTTDNLTKVTEEVVANMPEGLTPDLVQ EACESELNEVTGTKIAYETKMDLVQTSEVMQESLYPAAQLCPSFEESEATPSPVLPDIVMEAPLNSAVPS AGASVIQPSSSPLEASSVNYESIKHEPENPPPYEEAMSVSLKKVSGIKEEIKEPENINAALQETEAPYIS IACDLIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSDDSIPDVPQKQDETVML VKESLTETSFESMIEYENKEKLSALPPEGGKPYLESFKLSLDNTKDTLLPDEVSTLSKKEKIPLQMEELS TAVYSNDDLFISKEAQIRETETFSDSSPIEIIDEFPTLISSKTDSFSKLAREYTDLEVSHKSEIANAPDG AGSLPCTELPHDLSLKNIQPKVEEKISFSDDFSKNGSATSKVLLLPPDVSALATQAEIESIVKPKVLVKE AEKKLPSDTEKEDRSPSAIFSAELSKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALA LLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLV DSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPG LKRKAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_207521 |
| ORF Size | 2958 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_207521.2 |
| RefSeq Size | 4129 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 57142 |
| UniProt ID | Q9NQC3 |
| Protein Families | Transmembrane |
| MW | 108.4 kDa |
| Gene Summary | This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221136L3 | Lenti-ORF clone of RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 5 |
CNY 9,030.00 |
|
| RC221136L4 | Lenti-ORF clone of RTN4 (mGFP-tagged)-Human reticulon 4 (RTN4), transcript variant 5 |
CNY 9,030.00 |
|
| RG221136 | RTN4 (tGFP-tagged) - Human reticulon 4 (RTN4), transcript variant 5 |
CNY 7,890.00 |
|
| SC308608 | RTN4 (untagged)-Human reticulon 4 (RTN4), transcript variant 5 |
CNY 7,220.00 |
