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ARAP3 (NM_022481) Human Tagged ORF Clone
CAT＃: RC221115
ARAP3 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3)
ORF Plasmid: tGFP
"NM_022481" in other vectors (6)
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CNY 10,568.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CENTD3; DRAG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221115 representing NM_022481
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCTCAGGACCTGGACATCGCTGTGTGGCTGGCCACGGTGCACCTGGAGCAGTATGCAGACA CGTTCCGACGGCATGGCCTGGCTACAGCAGGTGCAGCCCGGGGCCTGGGCCACGAGGAGTTGAAGCAGTT GGGCATCAGCGCCACAGGGCACCGGAAACGCATTCTACGCCTGCTGCAGACAGGCACCGAAGAGGGCTCC CTGGATCCCAAATCAGATAGTGCCATGGAACCATCCCCCAGCCCAGCCCCGCAAGCCCAGCCCCCTAAGC CCGTGCCGAAGCCCAGGACCGTGTTTGGTGGACTCAGTGGCCCTGCCACCACTCAGAGACCTGGGCTGAG CCCAGCCCTCGGGGGACCAGGAGTGTCCAGGAGCCCAGAGCCCAGCCCAAGGCCCCCTCCTCTCCCCACT TCCTCCTCTGAGCAGTCTTCAGCCCTAAATACTGTGGAGATGATGCCTAATTCCATCTACTTCGGCCTGG ACTCAAGAGGCAGGGCACAGGCAGCTCAGGACAAGGCCCCAGACAGCTCCCAAATCTCTGCCCCCACCCC TGCCCTCAGGCCCACAACAGGCACAGTGCACATCATGGATCCTGGTTGCCTGTACTATGGTGTCCAACCT GTGGGGACTCCAGGAGCCCCCGACAGAAGAGAGAGCAGAGGTGTTTGTCAGGGCAGGGCTGAACACAGGC TCAGCAGACAGGATCTGGAGGCACGGGAGGATGCTGGCTATGCCAGCCTTGAGCTACCTGGAGACTCCAC CCTCTTATCGCCCACCCTGGAAACAGAGGAGACCAGTGATGACCTCATTTCACCCTATGCCAGCTTCTCC TTCACGGCAGACCGCCTCACGCCCCTGCTCAGTGGCTGGCTAGACAAGCTCTCCCCTCAGGGAAACTATG TCTTCCAGAGACGCTTTGTGCAGTTCAATGGGAGGAGTCTGATGTACTTTGGCAGTGACAAGGACCCCTT CCCTAAGGGTGTGATACCTTTGACTGCCATTGAGATGACCCGCAGCAGCAAGGACAACAAGTTCCAGGTC ATCACCGGCCAGAGGGTGTTCGTGTTCCGCACAGAGAGCGAGGCTCAGCGGGACATGTGGTGCTCCACGC TGCAGTCCTGTCTGAAGGAGCAGCGCCTCCTGGGCCACCCCCGGCCCCCCCAACCACCCCGACCCCTCCG CACGGGCATGCTGGAGCTGCGTGGACACAAGGCCAAGGTGTTTGCTGCCTTGAGCCCTGGAGAGCTGGCA CTGTACAAGAGTGAGCAGGCCTTCTCTCTGGGCATCGGGATCTGCTTCATCGAACTGCAGGGCTGCAGCG TCCGGGAGACCAAGAGTCGAAGCTTTGACCTGCTCACACCCCATCGCTGCTTCAGCTTCACAGCCGAGTC TGGGGGTGCTCGGCAGAGCTGGGCGGCCGCTCTGCAGGAAGCAGTAACCGAGACCCTGTCTGACTACGAG GTGGCTGAGAAGATCTGGTCTAATCGGGCCAACCGGCAGTGTGCGGACTGTGGGTCCTCCCGCCCAGATT GGGCTGCTGTCAATTTGGGGGTGGTCATCTGCAAGCAGTGTGCAGGTCAGCACCGGGCCCTGGGTTCTGG GATCTCCAAGGTGCAGAGCCTGAAGCTGGACACGAGTGTCTGGAGTAATGAGATAGTACAGTTATTCATT GTCCTGGGAAATGATCGTGCCAACCGCTTCTGGGCAGGGACCCTACCCCCAGGTGAGGGACTACATCCAG ATGCGACCCCTGGCCCCCGGGGAGAGTTCATCTCCCGAAAGTACCGTCTGGGTCTCTTCCGGAAGCCCCA CCCTCAGTACCCAGATCATAGCCAGCTTCTCCAGGCACTGTGTGCAGCTGTGGCAAGACCCAACCTGCTG AAGAACATGACCCAGCTCCTCTGTGTTGAGGCCTTTGAAGGCGAGGAGCCCTGGTTCCCCCCAGCCCCTG ATGGCAGCTGCCCTGGCCTCTTGCCCTCAGACCCCTCCCCTGGTGTGTACAATGAGGTGGTGGTGCGTGC TACTTACAGCGGCTTCCTGTACTGCAGTCCCGTCAGCAACAAAGCTGGACCCTCACCCCCTCGCAGGGGC CGGGATGCTCCCCCGCGCCTTTGGTGTGTGCTGGGAGCAGCTCTGGAAATGTTTGCATCGGAAAACAGCC CTGAACCCCTCAGCCTCATACAGCCCCAGGATATTGTATGTCTGGGTGTGAGCCCCCCACCCACTGACCC AGGTGACAGGTTCCCCTTTTCCTTTGAGCTCATCCTCGCTGGGGGGAGGATCCAGCATTTTGGCACAGAT GGAGCTGACAGTCTGGAGGCCTGGACTAGTGCTGTGGGCAAGTGGTTCTCCCCGCTGAGCTGCCACCAGC TGCTGGGCCCCGGGCTGCTGCGGCTGGGCCGCCTATGGCTGCGGTCCCCCTCCCATACAGCCCCGGCCCC TGGTCTCTGGCTGTCAGGGTTTGGCCTCCTTCGTGGTGACCACCTCTTCCTGTGCTCAGCGCCGGGCCCA GGCCCCCCAGCCCCTGAGGACATGGTGCATCTGCGGCGGCTACAGGAGATCAGTGTGGTTTCTGCAGCTG ACACCCCAGATAAGAAAGAGCATTTGGTCCTGGTGGAGACAGGAAGGACCCTGTATCTGCAAGGAGAGGG CCGGCTGGACTTCACGGCATGGAACGCAGCCATTGGGGGCGCGGCTGGTGGGGGCGGCACAGGGCTGCAG GAGCAGCAGATGAGCCGGGGTGACATCCCCATCATCGTGGATGCCTGCATCAGTTTTGTTACCCAGCATG GGCTCCGGCTGGAAGGTGTATACCGGAAAGGGGGCGCTCGTGCCCGCAGCCTGAGACTCCTGGCTGAGTT CCGTCGGGATGCCCGGTCGGTGAAGCTCCGACCAGGGGAGCACTTTGTGGAGGATGTCACTGACACACTC AAACGCTTCTTTCGTGAGCTCGATGACCCTGTGACCTCTGCACGGTTGCTGCCTCGCTGGAGGGAGGCTG CTGAGCTGCCCCAGAAGAATCAGCGCCTGGAGAAATATAAAGATGTGATTGGCTGCCTGCCGCGGGTCAA CCGCCGCACACTGGCCACCCTCATTGGGCATCTCTATCGGGTGCAGAAATGTGCGGCTCTAAACCAGATG TGCACGCGGAACTTGGCTCTGCTGTTTGCACCCAGCGTGTTCCAGACGGATGGGCGAGGGGAGCACGAGG TGCGAGTGCTGCAAGAGCTCATTGATGGCTACATCTCTGTCTTTGATATCGATTCTGACCAGGTAGCTCA GATTGACTTGGAGGTCAGTCTTATCACCACCTGGAAGGACGTGCAGCTGTCTCAGGCTGGAGACCTCATC ATGGAAGTTTATATAGAGCAGCAGCTCCCAGACAACTGTGTCACCCTGAAGGTGTCCCCAACCCTGACTG CTGAGGAGCTGACTAACCAGGTACTGGAGATGCGGGGGACAGCAGCTGGGATGGACTTGTGGGTGACTTT TGAGATTCGCGAGCATGGGGAGCTGGAGCGGCCACTGCATCCCAAGGAAAAGGTCTTAGAGCAGGCTTTA CAATGGTGCCAGCTCCCAGAGCCCTGCTCAGCCTCCCTGCTCTTGAAAAAAGTCCCCCTGGCCCAAGCTG GCTGCCTCTTCACAGGTATCCGACGTGAGAGCCCACGGGTGGGGCTGTTGCGGTGTCGTGAGGAGCCACC TCGCTTGCTGGGAAGCCGCTTCCAGGAGAGGTTCTTTCTGCTGCGTGGCCGCTGCCTGCTGCTGCTCAAG GAGAAGAAAAGCTCTAAACCAGAACGGGAGTGGCCTTTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCA AGAAGTTAAAGCCCCCAACACCGTGGGGCTTCACATTGATACTAGAGAAGATGCACCTCTACTTGTCCTG CACTGACGAGGATGAAATGTGGGATTGGACCACCAGCATCCTTAAAGCCCAGCACGATGACCAGCAGCCA GTGGTCTTACGACGCCATTCCTCCTCTGACCTTGCCCGTCAGAAGTTTGGCACTATGCCTTTGCTGCCTA TCCGTGGGGATGACAGTGGAGCCACCCTCCTCTCTGCCAATCAGACCCTGCGGCGACTACACAACCGGAG GACCCTGTCCATGTTCTTTCCAATGAAGTCATCCCAGGGGTCTGTGGAGGAGCAAGAGGAGCTGGAGGAG CCTGTGTACGAGGAGCCAGTGTATGAGGAAGTAGGGGCCTTCCCTGAGTTGATCCAGGACACTTCTACCT CCTTCTCCACCACACGGGAGTGGACAGTGAAGCCAGAGAACCCCCTCACCAGCCAGAAGTCATTGGATCA ACCCTTTCTCTCCAAGTCAAGCACCCTTGGCCAGGAGGAGAGGCCACCTGAGCCCCCTCCAGGCCCCCCT TCAAAGAGCAGTCCCCAGGCACGGGGGTCCCTAGAGGAACAGCTGCTCCAGGAGCTCAGCAGCCTCATCC TGAGGAAAGGAGAGACCACTGCAGGCCTGGGAAGTCCTTCCCAGCCATCCAGCCCCCAATCCCCCAGCCC CACTGGCCTTCCAACACAGACACCTGGCTTCCCCACCCAACCCCCATGCACTTCCAGTCCACCCTCCAGC CAGCCCCTCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221115 representing NM_022481
Red=Cloning site Green=Tags(s) MAAPQDLDIAVWLATVHLEQYADTFRRHGLATAGAARGLGHEELKQLGISATGHRKRILRLLQTGTEEGS LDPKSDSAMEPSPSPAPQAQPPKPVPKPRTVFGGLSGPATTQRPGLSPALGGPGVSRSPEPSPRPPPLPT SSSEQSSALNTVEMMPNSIYFGLDSRGRAQAAQDKAPDSSQISAPTPALRPTTGTVHIMDPGCLYYGVQP VGTPGAPDRRESRGVCQGRAEHRLSRQDLEAREDAGYASLELPGDSTLLSPTLETEETSDDLISPYASFS FTADRLTPLLSGWLDKLSPQGNYVFQRRFVQFNGRSLMYFGSDKDPFPKGVIPLTAIEMTRSSKDNKFQV ITGQRVFVFRTESEAQRDMWCSTLQSCLKEQRLLGHPRPPQPPRPLRTGMLELRGHKAKVFAALSPGELA LYKSEQAFSLGIGICFIELQGCSVRETKSRSFDLLTPHRCFSFTAESGGARQSWAAALQEAVTETLSDYE VAEKIWSNRANRQCADCGSSRPDWAAVNLGVVICKQCAGQHRALGSGISKVQSLKLDTSVWSNEIVQLFI VLGNDRANRFWAGTLPPGEGLHPDATPGPRGEFISRKYRLGLFRKPHPQYPDHSQLLQALCAAVARPNLL KNMTQLLCVEAFEGEEPWFPPAPDGSCPGLLPSDPSPGVYNEVVVRATYSGFLYCSPVSNKAGPSPPRRG RDAPPRLWCVLGAALEMFASENSPEPLSLIQPQDIVCLGVSPPPTDPGDRFPFSFELILAGGRIQHFGTD GADSLEAWTSAVGKWFSPLSCHQLLGPGLLRLGRLWLRSPSHTAPAPGLWLSGFGLLRGDHLFLCSAPGP GPPAPEDMVHLRRLQEISVVSAADTPDKKEHLVLVETGRTLYLQGEGRLDFTAWNAAIGGAAGGGGTGLQ EQQMSRGDIPIIVDACISFVTQHGLRLEGVYRKGGARARSLRLLAEFRRDARSVKLRPGEHFVEDVTDTL KRFFRELDDPVTSARLLPRWREAAELPQKNQRLEKYKDVIGCLPRVNRRTLATLIGHLYRVQKCAALNQM CTRNLALLFAPSVFQTDGRGEHEVRVLQELIDGYISVFDIDSDQVAQIDLEVSLITTWKDVQLSQAGDLI MEVYIEQQLPDNCVTLKVSPTLTAEELTNQVLEMRGTAAGMDLWVTFEIREHGELERPLHPKEKVLEQAL QWCQLPEPCSASLLLKKVPLAQAGCLFTGIRRESPRVGLLRCREEPPRLLGSRFQERFFLLRGRCLLLLK EKKSSKPEREWPLEGAKVYLGIRKKLKPPTPWGFTLILEKMHLYLSCTDEDEMWDWTTSILKAQHDDQQP VVLRRHSSSDLARQKFGTMPLLPIRGDDSGATLLSANQTLRRLHNRRTLSMFFPMKSSQGSVEEQEELEE PVYEEPVYEEVGAFPELIQDTSTSFSTTREWTVKPENPLTSQKSLDQPFLSKSSTLGQEERPPEPPPGPP SKSSPQARGSLEEQLLQELSSLILRKGETTAGLGSPSQPSSPQSPSPTGLPTQTPGFPTQPPCTSSPPSS QPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022481 |
| ORF Size | 4632 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022481.6 |
| RefSeq Size | 5281 bp |
| RefSeq ORF | 4635 bp |
| Locus ID | 64411 |
| UniProt ID | Q8WWN8 |
| Protein Pathways | Endocytosis |
| MW | 169.7 kDa |
| Gene Summary | This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221115L1 | Lenti ORF clone of Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3), Myc-DDK-tagged |
CNY 12,968.00 |
|
| RC221115L2 | Lenti ORF clone of Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3), mGFP tagged |
CNY 12,968.00 |
|
| RC221115L3 | Lenti ORF clone of Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3), Myc-DDK-tagged |
CNY 15,680.00 |
|
| RC221115L4 | Lenti ORF clone of Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3), mGFP tagged |
CNY 12,968.00 |
|
| RG221115 | ARAP3 (tGFP-tagged) - Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3) |
CNY 15,200.00 |
|
| SC305027 | ARAP3 (untagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (ARAP3) |
CNY 29,640.00 |
