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cGKI (PRKG1) (NM_006258) Human Tagged ORF Clone
CAT＃: RC220986
PRKG1 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2
ORF Plasmid: tGFP
"NM_006258" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220986 representing NM_006258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCTTGCGGGATTTACAGTACGCGCTCCAGGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCTC TCATCGACGAGCTGGAGCTGGAGTTGGATCAGAAGGACGAACTGATCCAGAAGCTGCAGAACGAGCTGGA CAAGTACCGCTCGGTGATCCGACCAGCCACCCAGCAGGCGCAGAAGCAGAGCGCGAGCACCTTGCAGGGC GAGCCGCGCACCAAGCGGCAGGCGATCTCCGCCGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCATG TGACCCTGCCCTTCTACCCCAAGAGCCCACAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGA CTTTATGAAGAACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTACCCGGTGGAGTATGGC AAGGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTGTATGCCATGGAAGATGGTAAGGTTG AAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCCAGGAAAAGTGTTTGGGGAATTGGCTATTCT TTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTAAATGTAAAACTCTGGGCCATTGATCGACAA TGTTTTCAAACAATAATGATGAGGACAGGACTCATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCG TTCCAACATTCCAGAGCCTTCCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACCCACTA TGAAAATGGAGAATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATCATCAGCAAAGGAACG GTAAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTTCTTAGAACTTTAGGAAAAGGAGACT GGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTGAGAACAGCAAACGTAATTGCTGCAGAAGCTGTAAC CTGCCTTGTGATTGACAGAGACTCTTTTAAACATTTGATTGGAGGGCTGGATGATGTTTCTAATAAAGCA TATGAAGATGCAGAAGCTAAAGCAAAATATGAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTG ATTTCAACATCATTGATACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAAAGTGA AGAATCCAAAACGTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACAAGACAGCAGGAGCAC ATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTCATAGTGAGACTGTACAGAACATTTA AGGACAGCAAATATTTGTATATGTTGATGGAAGCTTGTCTAGGTGGAGAGCTCTGGACCATTCTCAGGGA TAGAGGTTCGTTTGAAGATTCTACAACCAGATTTTACACAGCATGTGTGGTAGAAGCTTTTGCCTATCTG CATTCCAAAGGAATCATTTACAGGGACCTCAAGCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCA AACTGGTTGATTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCC AGAGTATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGACTACTGGTCACTGGGA ATCCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGCCCAGATCCTATGAAAACCTATAACA TCATATTGAGGGGGATTGACATGATAGAATTTCCAAAGAAGATTGCCAAAAATGCTGCTAATTTAATTAA AAAACTATGCAGGGACAATCCATCAGAAAGATTAGGGAATTTGAAAAATGGAGTAAAAGACATTCAAAAG CACAAATGGTTTGAGGGCTTTAACTGGGAAGGCTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAA GTGTTGCATCACCCACAGACACAAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCACCTGA TGACAACTCAGGATGGGATATAGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220986 representing NM_006258
Red=Cloning site Green=Tags(s) MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQG EPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYG KDSCIIKEGDVGSLVYAMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQ CFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGT VNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKA YEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEH IRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYL HSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLG ILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQK HKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006258 |
| ORF Size | 2058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006258.4 |
| RefSeq Size | 3740 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 5592 |
| UniProt ID | Q13976 |
| Domains | cNMP, pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Gap junction, Long-term depression, Olfactory transduction, Vascular smooth muscle contraction |
| MW | 77.6 kDa |
| Gene Summary | Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
HIV-1 exposure promotes PKG1-mediated phosphorylation and degradation of stathmin to increase epithelial barrier permeability
,Xie, W;Chen, M;Zhai, Z;Li, H;Song, T;Zhu, Y;Dong, D;Zhou, P;Duan, L;Zhang, Y;Li, D;Liu, X;Zhou, J;Liu, M;,
The Journal of biological chemistry
,PubMed ID 33839152
[cGKI]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220986L1 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220986L2 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC220986L3 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220986L4 | Lenti ORF clone of Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG220986 | PRKG1 (tGFP-tagged) - Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2 |
CNY 4,370.00 |
|
| SC116252 | PRKG1 (untagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2 |
CNY 5,032.00 |
