MT (MCAT) (NM_014507) Human Tagged ORF Clone

CAT#: RC220973

MCAT (Myc-DDK-tagged)-Human malonyl CoA:ACP acyltransferase (mitochondrial) (MCAT), nuclear gene encoding mitochondrial protein, transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_014507" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


MCAT mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms fabD; FASN2C; MCT; MCT1; MT; NET62
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220973 representing NM_014507
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGTCCGGGTCGCACGGGTAGCGTGGGTCAGGGGCTTGGGCGCCAGCTACCGCCGCGGCGCCTCGA
GCTTCCCGGTGCCTCCGCCGGGCGCCCAGGGTGTAGCGGAGCTGCTGCGAGATGCGACCGGGGCGGAGGA
GGAGGCGCCCTGGGCGGCGACGGAGCGGCGAATGCCGGGCCAGTGCTCCGTGCTGCTCTTCCCGGGCCAG
GGCAGCCAGGTGGTGGGCATGGGCCGCGGTCTGCTCAACTACCCGCGCGTCCGCGAACTCTACGCCGCCG
CCCGCCGCGTGCTGGGCTACGACCTGCTGGAACTGAGCCTGCACGGGCCGCAGGAGACCCTGGACCGCAC
CGTGCACTGTCAGCCCGCGATCTTCGTGGCATCGCTGGCCGCTGTCGAGAAACTACATCACCTGCAGCCC
TCGGTGATTGAGAACTGTGTTGCTGCTGCTGGATTCAGTGTGGGAGAGTTTGCAGCCCTAGTGTTTGCCG
GAGCCATGGAATTTGCTGAAGGCTCTACGGTTTCTCCAGAAGAATTCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220973 representing NM_014507
Red=Cloning site Green=Tags(s)

MSVRVARVAWVRGLGASYRRGASSFPVPPPGAQGVAELLRDATGAEEEAPWAATERRMPGQCSVLLFPGQ
GSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQPAIFVASLAAVEKLHHLQP
SVIENCVAAAGFSVGEFAALVFAGAMEFAEGSTVSPEEFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014507
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014507.3
RefSeq Size 1174 bp
RefSeq ORF 543 bp
Locus ID 27349
UniProt ID Q8IVS2
Protein Pathways Fatty acid biosynthesis, Metabolic pathways
MW 19.18 kDa
Gene Summary The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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