COL11A1 (NM_080629) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CO11A1; COLL6; DFNA37; STL2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC220895 representing NM_080629 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGTGGTCCTCTAGGTGGAAAACGAAACGGTGGCTCTGGGATTTCACCGTAACAACCCTCGCAT TGACCTTCCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATGTACTAAAAGCACTAGATTT TCACAATTCTCCAGAGGGAATATCAAAAACAACGGGATTTTGCACAAACAGAAAGAATTCTAAAGGCTCA GATACTGCTTACAGAGTTTCAAAGCAAGCACAACTCAGTGCCCCAACAAAACAGTTATTTCCAGGTGGAA CTTTCCCAGAAGACTTTTCAATACTATTTACAGTAAAACCAAAAAAAGGAATTCAGTCTTTCCTTTTATC TATATATAATGAGCATGGTATTCAGCAAATTGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTGTTTGAA GACCACACTGGAAAACCTGCCCCAGAAGACTATCCCCTCTTCAGAACTGTTAACATCGCTGACGGGAAGT GGCATCGGGTAGCAATCAGCGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAACCAC GAAACCACTTGATAGAAGTGAGAGAGCAATTGTTGATACCAATGGAATCACGGTTTTTGGAACAAGGATT TTGGATGAAGAAGTTTTTGAGGGGGACATTCAGCAGTTTTTGATCACAGGTGATCCCAAGGCAGCATATG ACTACTGTGAGCATTATAGTCCAGACTGTGACTCTTCAGCACCCAAGGCTGCTCAAGCTCAGGAACCTCA GATAGATGAGAAAAAGAAATCCAATTTCAAAAAGAAGATGAGGACAGTGGCTACTAAATCAAAGGAAAAA TCCAAAAAGTTTACACCCCCCAAATCTGAAAAATTTTCATCCAAGAAGAAGAAAAGTTATCAAGCATCAG CAAAAGCCAAACTAGGGGTAAAGGCAAACATCGTTGATGATTTTCAAGAATACAACTATGGAACAATGGA AAGTTACCAGACAGAAGCTCCTAGGCATGTTTCTGGGACAAATGAGCCAAATCCAGTTGAAGAAATATTT ACTGAAGAATATCTAACGGGAGAGGATTATGATTCCCAGAGGAAAAATTCTGAGGATACACTATATGAAA ACAAAGAAATAGACGGCAGGGATTCTGATCTTCTGGTAGATGGAGATTTAGGCGAATATGATTTTTATGA ATATAAAGAATATGAAGATAAACCAACAAGCCCCCCTAATGAAGAATTTGGTCCAGGTGTACCAGCAGAA ACTGATATTACAGAAACAAGCATAAATGGCCATGGTGCATATGGAGAGAAAGGACAGAAAGGAGAACCAG CAGTGGTTGAGCCTGGTATGCTTGTCGAAGGACCACCAGGACCAGCAGGACCTGCAGGTATTATGGGTCC TCCAGGTCTACAAGGCCCCACTGGACCCCCTGGTGACCCTGGCGATAGGGGCCCCCCAGGACGTCCTGGC TTACCAGGGGCTGATGGTCTACCTGGTCCTCCTGGTACTATGTTGATGTTACCGTTCCGTTATGGTGGTG ATGGTTCCAAAGGACCAACCATCTCTGCTCAGGAAGCTCAGGCTCAAGCTATTCTTCAGCAGGCTCGGAT TGCTCTGAGAGGCCCACCTGGCCCAATGGGTCTAACTGGAAGACCAGGTCCTGTGGGGGGGCCTGGTTCA TCTGGGGCCAAAGGTGAGAGTGGTGATCCAGGTCCTCAGGGCCCTCGAGGCGTCCAGGGTCCCCCTGGTC CAACGGGAAAACCTGGAAAAAGGGGTCGTCCAGGTGCAGATGGAGGAAGAGGAATGCCAGGAGAACCTGG GGCAAAGGGAGATCGAGGGTTTGATGGACTTCCGGGTCTGCCAGGTGACAAAGGTCACAGGGGTGAACGA GGTCCTCAAGGTCCTCCAGGTCCTCCTGGTGATGATGGAATGAGGGGAGAAGATGGAGAAATTGGACCAA GAGGTCTTCCAGGTGAAGCTGGCCCACGAGGTTTGCTGGGTCCAAGGGGAACTCCAGGAGCTCCAGGGCA GCCTGGTATGGCAGGTGTAGATGGCCCCCCAGGACCAAAAGGGAACATGGGTCCCCAAGGGGAGCCTGGG CCTCCAGGTCAACAAGGGAATCCAGGACCTCAGGGTCTTCCTGGTCCACAAGGTCCAATTGGTCCTCCTG GTGAAAAAGGACCACAAGGAAAACCAGGACTTGCTGGACTTCCTGGTGCTGATGGGCCTCCTGGTCATCC TGGGAAAGAAGGCCAGTCTGGAGAAAAGGGGGCTCTGGGTCCCCCTGGTCCACAAGGTCCTATTGGATAC CCGGGCCCCCGGGGAGTAAAGGGAGCAGATGGTGTCAGAGGTCTCAAGGGATCTAAAGGTGAAAAGGGTG AAGATGGTTTTCCAGGATTCAAAGGTGACATGGGTCTAAAAGGTGACAGAGGAGAAGTTGGTCAAATTGG CCCAAGAGGGGAAGATGGCCCTGAAGGACCCAAAGGTCGAGCAGGCCCAACTGGAGACCCAGGTCCTTCA GGTCAAGCAGGAGAAAAGGGAAAACTTGGAGTTCCAGGATTACCAGGATATCCAGGAAGACAAGGTCCAA AGGGTTCCACTGGATTCCCTGGGTTTCCAGGTGCCAATGGAGAGAAAGGTGCACGGGGAGTAGCTGGCAA ACCAGGCCCTCGGGGTCAGCGTGGTCCAACGGGTCCTCGAGGTTCAAGAGGTGCAAGAGGTCCCACTGGG AAACCTGGGCCAAAGGGCACTTCAGGTGGCGATGGCCCTCCTGGCCCTCCAGGTGAAAGAGGTCCTCAAG GACCTCAGGGTCCAGTTGGATTCCCTGGACCAAAAGGCCCTCCTGGACCACCAGGAAGGATGGGCTGCCC AGGACACCCTGGGCAACGTGGGGAGACTGGATTTCAAGGCAAGACCGGCCCTCCTGGGCCAGGGGGAGTG GTTGGACCACAGGGACCAACCGGTGAGACTGGTCCAATAGGGGAACGTGGGCATCCTGGCCCTCCTGGCC CTCCTGGTGAGCAAGGTCTTCCTGGTGCTGCAGGAAAAGAAGGTGCAAAGGGTGATCCAGGTCCTCAAGG TATCTCAGGGAAAGATGGACCAGCAGGATTACGTGGTTTCCCAGGGGAAAGAGGTCTTCCTGGAGCTCAG GGTGCACCTGGACTGAAAGGAGGGGAAGGTCCCCAGGGCCCACCAGGTCCAGTTGGCTCACCAGGAGAAC GTGGGTCAGCAGGTACAGCTGGCCCAATTGGTTTACCAGGGCGCCCGGGACCTCAGGGTCCTCCTGGTCC AGCTGGAGAGAAAGGTGCTCCTGGAGAAAAAGGTCCCCAAGGGCCTGCAGGGAGAGATGGAGTTCAAGGT CCTGTTGGTCTCCCAGGGCCAGCTGGTCCTGCCGGCTCCCCTGGGGAAGACGGAGACAAGGGTGAAATTG GTGAGCCGGGACAAAAAGGCAGCAAGGGTGACAAGGGAGAAAATGGCCCTCCCGGTCCCCCAGGTCTTCA AGGACCAGTTGGTGCCCCTGGAATTGCTGGAGGTGATGGTGAACCAGGTCCTAGAGGACAGCAGGGGATG TTTGGGCAAAAAGGTGATGAGGGTGCCAGAGGCTTCCCTGGACCTCCTGGTCCAATAGGTCTTCAGGGTC TGCCAGGCCCACCTGGTGAAAAAGGTGAAAATGGGGATGTTGGTCCATGGGGGCCACCTGGTCCTCCAGG CCCAAGAGGCCCTCAAGGTCCCAATGGAGCTGATGGACCACAAGGACCCCCAGGTTCTGTTGGTTCAGTT GGTGGTGTTGGAGAAAAGGGTGAACCTGGAGAAGCAGGAAACCCAGGGCCTCCTGGGGAAGCAGGTGTAG GCGGTCCCAAAGGAGAAAGAGGAGAGAAAGGGGAAGCTGGTCCACCTGGAGCTGCTGGACCTCCAGGTGC CAAGGGGCCGCCAGGTGATGATGGCCCTAAGGGTAACCCGGGTCCTGTTGGTTTTCCTGGAGATCCTGGT CCTCCTGGGGAACTTGGCCCTGCAGGTCAAGATGGTGTTGGTGGTGACAAGGGTGAAGATGGAGATCCTG GTCAACCGGGTCCTCCTGGCCCATCTGGTGAGGCTGGCCCACCAGGTCCTCCTGGAAAACGAGGTCCTCC TGGAGCTGCAGGTGCAGAGGGAAGACAAGGTGAAAAAGGTGCTAAGGGGGAAGCAGGTGCAGAAGGTCCT CCTGGAAAAACCGGCCCAGTCGGTCCTCAGGGACCTGCAGGAAAGCCTGGTCCAGAAGGTCTTCGGGGCA TCCCTGGTCCTGTGGGAGAACAAGGTCTCCCTGGAGCTGCAGGCCAAGATGGACCACCTGGTCCTATGGG ACCTCCTGGCTTACCTGGTCTCAAAGGTGACCCTGGCTCCAAGGGTGAAAAGGGACATCCTGGTTTAATT GGCCTGATTGGTCCTCCAGGAGAACAAGGGGAAAAAGGTGACCGAGGGCTCCCTGGAACTCAAGGATCTC CAGGAGCAAAAGGGGATGGGGGAATTCCTGGTCCTGCTGGTCCCTTAGGTCCACCTGGTCCTCCAGGCTT ACCAGGTCCTCAAGGCCCAAAGGGTAACAAAGGCTCTACTGGACCCGCTGGCCAGAAAGGTGACAGTGGT CTTCCAGGGCCTCCTGGGCCTCCAGGTCCACCTGGTGAAGTCATTCAGCCTTTACCAATCTTGTCCTCCA AAAAAACGAGAAGACATACTGAAGGCATGCAAGCAGATGCAGATGATAATATTCTTGATTACTCGGATGG AATGGAAGAAATATTTGGTTCCCTCAATTCCCTGAAACAAGACATCGAGCATATGAAATTTCCAATGGGT ACTCAGACCAATCCAGCCCGAACTTGTAAAGACCTGCAACTCAGCCATCCTGACTTCCCAGATGGTGAAT ATTGGATTGATCCTAACCAAGGTTGCTCAGGAGATTCCTTCAAAGTTTACTGTAATTTCACATCTGGTGG TGAGACTTGCATTTATCCAGACAAAAAATCTGAGGGAGTAAGAATTTCATCATGGCCAAAGGAGAAACCA GGAAGTTGGTTTAGTGAATTTAAGAGGGGAAAACTGCTTTCATACTTAGATGTTGAAGGAAATTCCATCA ATATGGTGCAAATGACATTCCTGAAACTTCTGACTGCCTCTGCTCGGCAAAATTTCACCTACCACTGTCA TCAGTCAGCAGCCTGGTATGATGTGTCATCAGGAAGTTATGACAAAGCACTTCGCTTCCTGGGATCAAAT GATGAGGAGATGTCCTATGACAATAATCCTTTTATCAAAACACTGTATGATGGTTGTACGTCCAGAAAAG GCTATGAAAAGACTGTCATTGAAATCAATACACCAAAAATTGATCAAGTACCTATTGTTGATGTCATGAT CAATGACTTTGGTGATCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTCTTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC220895 representing NM_080629 Red=Cloning site Green=Tags(s) MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGS DTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFE DHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRI LDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEPQIDEKKKSNFKKKMRTVATKSKEK SKKFTPPKSEKFSSKKKKSYQASAKAKLGVKANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIF TEEYLTGEDYDSQRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAE TDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDPGDRGPPGRPG LPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGS SGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHRGER GPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPG PPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGY PGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPS GQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARGPTG KPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGRMGCPGHPGQRGETGFQGKTGPPGPGGV VGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGLRGFPGERGLPGAQ GAPGLKGGEGPQGPPGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQG PVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEPGPRGQQGM FGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPWGPPGPPGPRGPQGPNGADGPQGPPGSVGSV GGVGEKGEPGEAGNPGPPGEAGVGGPKGERGEKGEAGPPGAAGPPGAKGPPGDDGPKGNPGPVGFPGDPG PPGELGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGAAGAEGRQGEKGAKGEAGAEGP PGKTGPVGPQGPAGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLI GLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPLGPPGPPGLPGPQGPKGNKGSTGPAGQKGDSG LPGPPGPPGPPGEVIQPLPILSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMG TQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKP GSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSYDKALRFLGSN DEEMSYDNNPFIKTLYDGCTSRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQNQKFGFEVGPVCFLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_080629
ORF Size	5454 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_080629.1, NP_542196.1
RefSeq Size	6355 bp
RefSeq ORF	5457 bp
Locus ID	1301
UniProt ID	P12107
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	178 kDa
Gene Summary	This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]

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