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UCK (UCK1) (NM_031432) Human Tagged ORF Clone
CAT＃: RC220876
UCK1 (Myc-DDK-tagged)-Human uridine-cytidine kinase 1 (UCK1), transcript variant 1
ORF Plasmid: tGFP
"NM_031432" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | URK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220876 representing NM_031432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCACCAGCGGCCCT TCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGGAAGTCGACCGTGTGTGAGAAGATCATGGAGTTGCT GGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGACAGGTTCTACAAGGTC CTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAATTTTGACCATCCAGATGCCTTTGATA ATGATTTGATGCACAGGACTCTGAAGAACATCGTGGAGGGCAAAACGGTGGAGGTGCCGACCTATGATTT TGTGACACACTCAAGGTTACCAGAGACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATC TTGGTGTTCTACAGCCAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACG TCAGGCTGTCTCGAAGAGTTCTCCGGGACGTGCGCCGAGGGAGGGACCTGGAGCAGATTCTGACGCAGTA CACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTTTGCCTGCCGACAAAGAAGTATGCCGATGTGATCATC CCACGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTGCAGCACATCCAGGACATTCTGAATGGTG ACATCTGCAAATGGCACCGAGGAGGGTCCAATGGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGA CCACCCTGGGATGCTGACCTCTGGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220876 representing NM_031432
Red=Cloning site Green=Tags(s) MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIMELLGQNEVEQRQRKVVILSQDRFYKV LTAEQKAKALKGQYNFDHPDAFDNDLMHRTLKNIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGI LVFYSQEIRDMFHLRLFVDTDSDVRLSRRVLRDVRRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVII PRGVDNMVAINLIVQHIQDILNGDICKWHRGGSNGRSYKRTFSEPGDHPGMLTSGKRSHLESSSRPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031432 |
| ORF Size | 831 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031432.5 |
| RefSeq Size | 2160 bp |
| RefSeq ORF | 834 bp |
| Locus ID | 83549 |
| UniProt ID | Q9HA47 |
| Domains | PRK |
| Protein Families | Druggable Genome |
| Protein Pathways | Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism |
| MW | 31.3 kDa |
| Gene Summary | This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
An optimized chemical-genetic method for cell-specific metabolic labeling of RNA
,Nainar, S;Cuthbert, BJ;Lim, NM;England, WE;Ke, K;Sophal, K;Quechol, R;Mobley, DL;Goulding, CW;Spitale, RC;,
Nat. Methods
,PubMed ID 32015544
[UCK]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220876L1 | Lenti ORF clone of Human uridine-cytidine kinase 1 (UCK1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC220876L2 | Lenti ORF clone of Human uridine-cytidine kinase 1 (UCK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC220876L3 | Lenti ORF clone of Human uridine-cytidine kinase 1 (UCK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220876L4 | Lenti ORF clone of Human uridine-cytidine kinase 1 (UCK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG220876 | UCK1 (tGFP-tagged) - Human uridine-cytidine kinase 1 (UCK1), transcript variant 1 |
CNY 4,000.00 |
|
| SC107347 | UCK1 (untagged)-Human uridine-cytidine kinase 1 (UCK1), transcript variant 1 |
CNY 2,400.00 |
