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NrCAM (NM_001037132) Human Tagged ORF Clone
CAT＃: RC220506
- TrueORF®
NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1
ORF Plasmid: tGFP
"NM_001037132" in other vectors (4)
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CNY 13,616.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220506 representing NM_001037132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCTTGAAGACTTGGTACAGCCTCCAAC CATCACCCAACAGTCTCCAAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCC AAAGGGAAACCGCCCCCAAGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTC TGGTCACCATGAAGCCTGGCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTA TGAAGGAGTCTATCAGTGTACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGC CCATCCAGATCACCATTGTGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAG TACTTCCCTGCAGACCCCCAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAG ACTTCCACAAAGTGAGAGAGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAG GACACCCGCGAAGACTATATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTA TTTCTGTGAAGGTGATTTCAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTT TTATGGTGCTAAATCAAGTAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAATGCAAGTAACAAA GAGGAATTAAGAGGAAATGTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACCCCAATTATTTACT GGGCAAAGGAAGATGGAATGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGAAAACCTTGCAGAT CATTCATGTTTCAGAAGCAGACTCTGGAAATTACCAATGTATAGCAAAAAACGCATTAGGAGCCATCCAC CATACCATTTCTGTTAGAGTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAATCTTGTGCTGTCCC CAGGAGAGGATGGGACCTTGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTAGCTGGTTAACAAA TGGAGTCCCAATAGAAATTGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATACCATTATTTTTTCA AATGTTCAAGAAAGATCAAGTGCAGTCTATCAGTGCAATGCCTCTAATGAATATGGATATTTACTGGCAA ACGCATTTGTAAATGTGCTGGCTGAGCCACCACGAATCCTCACACCTGCAAACACACTCTACCAGGTCAT TGCAAACAGGCCTGCTTTACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCATCGAGTGGTTTAAA GGAGCTAAAGGAAGTGCTCTTCATGAAGATATTTATGTTTTACATGAAAATGGAACTTTGGAAATTCCTG TGGCCCAAAAGGACAGTACAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGATGGCGAAGAATGA AGTTCACTTAGAAATCAAAGATCCTACATGGATCGTTAAACAGCCCGAATATGCAGTTGTGCAAAGAGGG AGCATGGTGTCCTTTGAATGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTCCTGTGGCTGAAGG ACAACAGGGAACTGCCCAGTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGGTAGCTGATGTCAG TGACGATGACAGCGGGACCTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTCCGCCAGCGCTGTG CTTAGCGTTGTTGCTCCTACTCCAACTCCAGCTCCCGTTTACGATGTCCCAAATCCTCCCTTTGACTTAG AACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAGGCGATGACAACAATAGCCCCAT TACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCTGTGGCACCACCAAACTGAAGTT TCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAACTACTCCTTCCGCGTGATGGCAG TGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCGTCTGAGCAGTATTTGACGAAAGCCTCAGAACCAGA TAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTTGGTGATTACGTGGAAGCCCTTG AATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGGCGCCAGAAAGATGGTGATGATG AATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAGGCACGCCAACCTTTGTTCCATA CCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCCAGCTGTAGTCATGGGACATTCT GGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTGGTGAACAGTACCTTAGCCGAGG TGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAGGCTATCGGATTTACTATTGGAA GACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGATCCTCACCTTCCAAGGCAGCAAG ACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTGAATGTCCGAGTGGTCAATGGGA AAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAGGAGTCCCCAGTGCTCCCTCGTC TTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGATCCACCGAGCCACCCGAATGGC ATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACATGAATTAGGCCCTCTGGTAGATT TGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATTTCAGCACTCGATATAAGTTTTA TTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGAAGCAGTAACAACTGTGGATGAA GCTGGTATTCTTCCACCTGATGTAGGTGCAGGCAAAGTTCAAGCAGTAAATCCCAGGATCAGCAATCTTA CTGCTGCAGCTGCTGAGACCTATGCCAATATCAGTTGGGAATATGAGGGACCAGAGCATGTGAACTTTTA TGTTGAATATGGTGTAGCAGGCAGCAAAGAAGAATGGAGAAAAGAAATTGTAAATGGTTCTCGGAGCTTC TTTGGGTTAAAGGGTCTAATGCCAGGAACAGCATACAAAGTTCGAGTTGGTGCTGTGGGGGACTCTGGTT TTGTGAGTTCAGAGGATGTGTTTGAGACAGGCCCAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCA GGGCTGGTTCATTGGTCTGATGTGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATC AGAAGAAACAAGGGTGGTAAATATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGC CTATGAAGGAAGATGATGGGACATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGG AAGTCGAACTCCTTCAGACAGGACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAA GGGGTTAATGGCCAGTTCAATGAGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGC CGGCTGAAGGAAACGAAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220506 representing NM_001037132
Red=Cloning site Green=Tags(s) MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDPKLLEDLVQPPTITQQSPKDYIIDPRENIVIQCEA KGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVR PSRSPLWTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPE DTREDYICYARFNHTQTIQQKQPISVKVISVDELNDTIAANLSDTEFYGAKSSRERPPTFLTPEGNASNK EELRGNVLSLECIAEGLPTPIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIH HTISVRVKAAPYWITAPQNLVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFS NVQERSSAVYQCNASNEYGYLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFK GAKGSALHEDIYVLHENGTLEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDPTWIVKQPEYAVVQRG SMVSFECKVKHDHTLSLTVLWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAV LSVVAPTPTPAPVYDVPNPPFDLELTDQLDKSVQLSWTPGDDNNSPITKFIIEYEDAMHKPGLWHHQTEV SGTQTTAQLKLSPYVNYSFRVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKPL NGFESNGPGLQYKVSWRQKDGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDMGFAPEPAVVMGHS GEDLPMVAPGNVRVNVVNSTLAEVHWDPVPLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSK THGMLPGLEPFSHYTLNVRVVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNG ILTEYTLKYQPINSTHELGPLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSAGSGSQITEEAVTTVDE AGILPPDVGAGKVQAVNPRISNLTAAAAETYANISWEYEGPEHVNFYVEYGVAGSKEEWRKEIVNGSRSF FGLKGLMPGTAYKVRVGAVGDSGFVSSEDVFETGPAMASRQVDIATQGWFIGLMCAVALLILILLIVCFI RRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDRTVKKEDSDDSLVDYGE GVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001037132 |
| ORF Size | 3912 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001037132.4 |
| RefSeq Size | 6305 bp |
| RefSeq ORF | 3915 bp |
| Locus ID | 4897 |
| UniProt ID | Q92823 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 144.3 kDa |
| Gene Summary | Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220506L3 | Lenti-ORF clone of NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1 |
CNY 14,920.00 |
|
| RC220506L4 | Lenti-ORF clone of NRCAM (mGFP-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1 |
CNY 14,920.00 |
|
| RG220506 | NRCAM (tGFP-tagged) - Human neuronal cell adhesion molecule (NRCAM), transcript variant 1 |
CNY 14,350.00 |
|
| SC302851 | NRCAM (untagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 1 |
CNY 13,632.00 |
