OLAH (NM_018324) Human Tagged ORF Clone

CAT#: RC219627

OLAH (Myc-DDK-tagged)-Human oleoyl-ACP hydrolase (OLAH), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_018324" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-OLAH Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AURA1; SAST; TE2; THEDC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219627 representing NM_018324
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGGAGACCAACCTAAGAGAACCAGGAATGAAAACATTTTCAACTGCTTATACAAAAACCCTG
AGGCAACTTTTAAGCTGATTTGCTTTCCCTGGATGGGAGGTGGCTCCACTCATTTTGCCAAATGGGGCCA
AGATACTCATGATTTGCTGGAAGAGACAGCATCTCACCATGTTGCCAAGGCTGGTCTCAAACTCCGGCGC
TCAAGTGATCCTCCTGCTTCAGCCTACCCATGTGCTGGCGTGAGCCACCGTAGGCGTGAGCCACCGTGCC
TGGCCAAAATTCTTGGTCTATTCTGGATTCTAATTTTTTTTATGCACTCCTTAAGGCTTCCTGGAAGAGA
AAGCAGAGTTGAAGAACCTCTTGAAAATGACATCTCCCAGTTAGTTGATGAAGTTGTTTGTGCTCTGCAG
CCAGTCATCCAGGATAAACCATTTGCATTTTTTGGCCACAGTATGGGATCCTACATTGCTTTTAGGACTG
CACTAGGTCTAAAAGAAAACAATCAACCAGAACCATTGCATTTATTTTTGTCAAGTGCAACTCCTGTACA
TTCAAAGGCCTGGCATCGCATTCCCAAAGATGATGAATTGTCAGAAGAACAAATAAGTCATTACCTTATG
GAATTTGGAGGCACCCCCAAGCATTTTGCTGAAGCCAAGGAATTTGTGAAACAATGTAGTCCCATCATAA
GGGCAGATCTGAACATTGTTAGAAGTTGCACCTCTAACGTACCATCTAAGGCTGTTCTTTCCTGTGACTT
GACATGTTTTGTTGGATCTGAAGACATAGCAAAGGACATGGAAGCCTGGAAAGATGTAACCAGTGGAAAT
GCTAAAATTTACCAGCTTCCAGGGGGTCACTTTTATCTTCTGGATCCTGCGAACGAGAAATTAATCAAGA
ACTACATAATCAAGTGTCTAGAAGTATCATCGATATCCAATTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219627 representing NM_018324
Red=Cloning site Green=Tags(s)

MERGDQPKRTRNENIFNCLYKNPEATFKLICFPWMGGGSTHFAKWGQDTHDLLEETASHHVAKAGLKLRR
SSDPPASAYPCAGVSHRRREPPCLAKILGLFWILIFFMHSLRLPGRESRVEEPLENDISQLVDEVVCALQ
PVIQDKPFAFFGHSMGSYIAFRTALGLKENNQPEPLHLFLSSATPVHSKAWHRIPKDDELSEEQISHYLM
EFGGTPKHFAEAKEFVKQCSPIIRADLNIVRSCTSNVPSKAVLSCDLTCFVGSEDIAKDMEAWKDVTSGN
AKIYQLPGGHFYLLDPANEKLIKNYIIKCLEVSSISNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018324
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018324.3
RefSeq Size 1765 bp
RefSeq ORF 957 bp
Locus ID 55301
UniProt ID Q9NV23
Protein Pathways Fatty acid biosynthesis, Metabolic pathways
MW 35.6 kDa
Gene Summary Contributes to the release of free fatty acids from fatty acid synthase (FASN). Has broad substrate specificity, giving rise to a range of free fatty acids with chain lengths between 10 and 16 carbon atoms (C10 - C16).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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