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PIAS1 (NM_016166) Human Tagged ORF Clone
CAT＃: RC219594
PIAS1 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 1 (PIAS1)
ORF Plasmid: tGFP
"NM_016166" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DDXBP1; GBP; GU/RH-II; ZMIZ3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219594 representing NM_016166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAAGTACTGTTGG GCTACGCCGGGAGAAACAAGCACGGACGCAAACACGAACTTCTCACAAAAGCCCTGCATTTGCTAAAGGC TGGCTGTAGTCCTGCTGTGCAAATGAAAATTAAGGAACTCTATAGGCGGCGGTTCCCACAGAAAATCATG ACGCCTGCAGACTTGTCCATCCCCAACGTACATTCAAGTCCTATGCCAGCAACTTTGTCTCCATCTACCA TTCCACAACTCACTTACGATGGTCACCCTGCATCATCACCATTACTCCCTGTTTCTCTTCTGGGACCTAA ACATGAACTGGAACTCCCACATCTTACATCAGCTCTTCACCCAGTCCATCCGGATATAAAACTTCAAAAA TTACCATTTTATGATTTACTGGATGAACTGATAAAACCCACCAGTCTAGCATCAGACAACAGTCAGCGCT TTCGAGAAACCTGTTTTGCATTTGCCTTGACACCACAACAAGTGCAGCAAATCAGTAGTTCCATGGATAT TTCTGGGACCAAATGTGACTTCACAGTACAGGTCCAGTTAAGGTTTTGTTTATCAGAAACCAGTTGTCCA CAAGAAGATCACTTCCCACCCAATCTTTGTGTGAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACC TTCCACCTACAAAAAATGGCGTGGAACCAAAGCGACCCAGCCGACCAATTAATATCACCTCACTTGTCCG ACTGTCCACAACAGTACCAAACACGATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAAACTATTCCATG GCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAGGGAATAAGGA ATCCGGATCATTCTAGAGCTTTAATTAAAGAGAAGTTGACTGCGGATCCAGACAGTGAAATAGCTACAAC CAGCCTAAGGGTTTCTCTACTATGTCCACTTGGTAAAATGCGGCTGACAATTCCGTGTCGGGCCCTTACA TGTTCTCATCTACAATGTTTTGACGCAACTCTTTACATTCAGATGAATGAGAAAAAACCAACCTGGGTTT GTCCTGTCTGTGATAAGAAGGCTCCATATGAACACCTTATTATTGATGGCTTGTTTATGGAAATCCTAAA GTACTGTACAGACTGTGATGAAATACAATTTAAGGAGGATGGCACTTGGGCACCGATGAGATCAAAAAAG GAAGTACAGGAAGTTTCTGCCTCTTACAATGGAGTCGATGGATGCTTGAGCTCCACATTGGAGCATCAGG TAGCGTCTCACCACCAGTCCTCAAATAAAAACAAGAAAGTAGAAGTGATTGACCTAACCATAGACAGTTC ATCTGATGAAGAGGAAGAAGAGCCATCTGCCAAGAGGACCTGTCCTTCCCTATCTCCCACATCACCACTA AATAATAAAGGCATTTTAAGTCTTCCACATCAAGCATCTCCAGTATCCCGCACCCCAAGCCTTCCTGCTG TAGACACAAGCTACATTAATACCTCCCTCATCCAAGACTATAGGCATCCTTTCCACATGACACCCATGCC TTACGACTTACAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCTTG CTTGCCGCTGCAGCAGCAGCAGTTTCAGATGATCAAGACCTCCTACACTCGTCTCGGTTTTTCCCGTATA CCTCCTCACAGATGTTTCTTGATCAGTTAAGTGCAGGAGGCAGTACTTCTCTGCCAACCACCAATGGAAG CAGTAGTGGCAGTAACAGCAGCCTGGTTTCTTCCAACAGCCTAAGGGAAAGCCATAGCCACACCGTCACA AACAGGAGCAGCACGGACACGGCATCCATCTTTGGCATCATACCAGACATTATTTCATTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219594 representing NM_016166
Red=Cloning site Green=Tags(s) MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIM TPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQK LPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCP QEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSM AVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALT CSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGTWAPMRSKK EVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPL NNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSL LAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVT NRSSTDTASIFGIIPDIISLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016166 |
| ORF Size | 1953 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016166.3 |
| RefSeq Size | 2309 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 8554 |
| UniProt ID | O75925 |
| Domains | SAP, zf-MIZ |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| MW | 71.7 kDa |
| Gene Summary | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219594L1 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219594L2 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), mGFP tagged |
CNY 5,890.00 |
|
| RC219594L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219594L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), mGFP tagged |
CNY 5,890.00 |
|
| RG219594 | PIAS1 (tGFP-tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1) |
CNY 4,370.00 |
|
| SC114401 | PIAS1 (untagged)-Human protein inhibitor of activated STAT, 1 (PIAS1) |
CNY 4,856.00 |
