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METTL2B (NM_018396) Human Tagged ORF Clone
CAT＃: RC219439
METTL2B (Myc-DDK-tagged)-Human methyltransferase like 2B (METTL2B)
ORF Plasmid: tGFP
"NM_018396" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | METL; METTL2; METTL2A; PSENIP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219439 representing NM_018396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAGTCCTCGCCGATAAGAGGCAGCAGTTCGGAAGCCGGT TCCTGAGAGATCCGGCGCGCGTCTTCCACCACAATGCCTGGGACAATGTGGAGTGGTCGGAAGAGCAAGC CGCGGCGGCGGAGAGAAAAGTCCAGGAGAACAGTATCCAGCGGGTGTGCCAGGAGAAACAAGTTGATTAT GAGATCAATGCCCACAAATACTGGAATGACTTCTACAAAATCCACGAAAATGGGTTTTTCAAGGATAGAC ATTGGCTTTTTACCGAATTCCCTGAGCTGGCACCTAGCCAAAATCAAAATCATTTGAAGGACTGGTTCTT GGAGAACAAGAGTGAAGTACCTGAATGTAGAAACAATGAGGATGGACCTGGTTTAATAATGGAAGAACAG CACAAGTGTTCTTCGAAGAGCCTTGAACATAAAACACAGACACCTCCTGTGGAGGAGAATGTAACTCAGA AAATTAGTGACCTGGAAATTTGTGCTGATGAGTTTCCTGGATCCTCAGCCACCTACCGAATACTGGAGGT TGGCTGTGGTGTGGGAAACACAGTCTTTCCAATTTTACAAACGAACAATGACCCAGGACTCTTTGTTTAT TGCTGTGATTTTTCTTCCACAGCTATAGAACTGGTCCAGACAAATTCAGAATATGATCCTTCTCGGTGTT TTGCCTTTGTTCACGACCTGTGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGGCAGTCTTGATATTAT CATTCTCATATTTGTTCTTTCAGCAATTGTTCCAGACAAGATGCAGAAGGCTATCAACAGGCTGAGCAGG CTTCTGAAACCTGGCGGGATGATGCTTCTGCGAGATTACGGCCGCTATGACATGGCTCAGCTTCGGTTTA AAAAAGGTCAGTGTCTATCTGGAAATTTCTACGTGAGAGGTGATGGAACCAGAGTTTACTTCTTCACACA AGAGGAACTGGACACGCTTTTCACCACTGCTGGACTGGAAAAAGTTCAGAACCTGGTGGATCGCCGACTG CAGGTGAACCGAGGAAAGCAACTGACAATGTACCGGGTTTGGATTCAGTGCAAATACTGCAAGCCCCTTC TGTCCAGCACCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219439 representing NM_018396
Red=Cloning site Green=Tags(s) MAGSYPEGAPAVLADKRQQFGSRFLRDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQRVCQEKQVDY EINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVPECRNNEDGPGLIMEEQ HKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVY CCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSR LLKPGGMMLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRL QVNRGKQLTMYRVWIQCKYCKPLLSSTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018396 |
| ORF Size | 1134 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2192 bp |
| RefSeq ORF | 1137 bp |
| Locus ID | 55798 |
| UniProt ID | Q6P1Q9 |
| Protein Families | Druggable Genome |
| Protein Pathways | Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism |
| MW | 43.2 kDa |
| Gene Summary | This gene is a member of a family of methyltransferases that share homology with, but are distinct from, the UbiE family of methyltransferases. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The interactome of a family of potential methyltransferases in HeLa cells
,Ignatova, VV;Jansen, PWTC;Baltissen, MP;Vermeulen, M;Schneider, R;,
Sci Rep
,PubMed ID 31036863
[METTL2B]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219439L1 | Lenti ORF clone of Human methyltransferase like 2B (METTL2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219439L2 | Lenti ORF clone of Human methyltransferase like 2B (METTL2B), mGFP tagged |
CNY 5,890.00 |
|
| RC219439L3 | Lenti ORF clone of Human methyltransferase like 2B (METTL2B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219439L4 | Lenti ORF clone of Human methyltransferase like 2B (METTL2B), mGFP tagged |
CNY 5,890.00 |
|
| RG219439 | METTL2B (tGFP-tagged) - Human methyltransferase like 2B (METTL2B) |
CNY 4,370.00 |
|
| SC317606 | METTL2B (untagged)-Human methyltransferase like 2B (METTL2B) |
CNY 6,180.00 |
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