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GGA1 (NM_013365) Human Tagged ORF Clone
CAT＃: RC219134
GGA1 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 1
ORF Plasmid: tGFP
"NM_013365" in other vectors (4)
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CNY 4,760.00
CNY 7,505.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219134 representing NM_013365
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCGCGAATCAATAGAGCCACGAACCCCCTGAACAAGG AGCTCGACTGGGCCAGCATCAACGGCTTCTGCGAGCAGCTCAACGAGGACTTTGAGGGGCCTCCACTCGC CACCCGGCTGCTGGCCCACAAGATCCAGTCCCCACAGGAGTGGGAGGCGATCCAGGCCTTGACGGTGCTG GAAACATGCATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAACGAGC TCATCAAGGTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATCTTGGA GCTCCTCTACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATGCTAAAG AAGCAGGGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCTCCACGGC CGAAGAATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCCCATCC CGAAGACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAGAAG ATCTCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAGATGGTGATGA GCCACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGA GCGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCCTTAGCGGAGATC CTGCAGGCCAATGACAACCTCACCCAGGTGATCAACCTGTATAAGCAGCTGGTGCGGGGTGAGGAGGTCA ACGGTGATGCCACAGCCGGCTCCATCCCTGGGAGCACCTCGGCCCTGCTGGATCTCTCAGGCCTGGATCT CCCGCCTGCGGGCACCACCTACCCAGCTATGCCCACCCGCCCTGGCGAGCAGGCCAGCCCTGAGCAGCCC AGTGCCTCAGTTTCCCTGCTTGACGACGAGCTCATGTCTCTGGGCCTCAGTGACCCCACACCCCCTTCAG GCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAGTCGTCGGATGCCACTGAGCCCCCAGCCCCTGC TCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCAGCGCAGACATCCCTGCCAGCAAGCAGCGGTCTG GACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAGCAGTCGCTGCCCCCGGAATCCCAGCAAGTGCGGT GGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTCCGGGACCTGCAGAATAAGAGCAGCAGCTGCAGCTC CCCCAGCTCCAGCGCCACCAGCCTTCTCCACACCGTGTCCCCAGAGCCCCCCAGGCCTCCGCAGCAGCCC GTACCAACCGAGCTCTCACTGGCCAGCATCACTGTGCCCCTGGAGTCCATCAAACCCAGCAACATCCTGC CCGTGACTGTGTATGACCAGCACGGCTTCCGCATCCTCTTCCATTTTGCCCGGGACCCACTGCCAGGGCG CTCCGACGTGCTGGTGGTGGTGGTTTCCATGCTGAGCACCGCCCCCCAGCCCATCCGCAACATCGTGTTC CAGTCAGCTGTCCCCAAGGTTATGAAGGTGAAGCTGCAGCCACCCTCGGGCACGGAGCTGCCAGCTTTTA ACCCCATCGTCCACCCCTCAGCAATCACCCAGGTCCTGCTGCTTGCCAACCCCCAGAAGGAGAAGGTTCG CCTCCGCTACAAGCTCACCTTCACCATGGGTGACCAGACCTACAACGAGATGGGGGATGTGGACCAGTTC CCCCCACCTGAAACCTGGGGTAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219134 representing NM_013365
Red=Cloning site Green=Tags(s) MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVL ETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLK KQGIVKSDPKLPDDTTFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEK ISKRVNAIEEVNNNVKLLTEMVMSHSQGGAAAGSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEI LQANDNLTQVINLYKQLVRGEEVNGDATAGSIPGSTSALLDLSGLDLPPAGTTYPAMPTRPGEQASPEQP SASVSLLDDELMSLGLSDPTPPSGPSLDGTGWNSFQSSDATEPPAPALAQAPSMESRPPAQTSLPASSGL DDLDLLGKTLLQQSLPPESQQVRWEKQQPTPRLTLRDLQNKSSSCSSPSSSATSLLHTVSPEPPRPPQQP VPTELSLASITVPLESIKPSNILPVTVYDQHGFRILFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVF QSAVPKVMKVKLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLTFTMGDQTYNEMGDVDQF PPPETWGSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013365 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013365.5 |
| RefSeq Size | 3163 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 26088 |
| UniProt ID | Q9UJY5 |
| Domains | VHS, GAT, Alpha_adaptinC2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome |
| MW | 70.2 kDa |
| Gene Summary | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219134L3 | Lenti-ORF clone of GGA1 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 1 |
CNY 5,890.00 |
|
| RC219134L4 | Lenti-ORF clone of GGA1 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 1 |
CNY 5,890.00 |
|
| RG219134 | GGA1 (tGFP-tagged) - Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 1 |
CNY 4,370.00 |
|
| SC314296 | GGA1 (untagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 1 |
CNY 10,260.00 |
