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UBE3B (NM_130466) Human Tagged ORF Clone
CAT＃: RC219024
- TrueORF®
UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1
ORF Plasmid: tGFP
"NM_130466" in other vectors (6)
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CNY 7,440.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BPIDS; KOS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219024 representing NM_130466
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACCCTGTCTCAGACCTCGAGAGCATGGTTCATCGATAGAGCCCGTCAGGCACGAGAAGAAAGGC TTGTGCAGAAGGAACGGGAGCGGGCAGCTGTTGTGATCCAGGCCCATGTCCGGAGTTTTCTCTGTCGGAG TCGACTGCAGAGAGATATCAGGAGAGAGATTGATGACTTTTTTAAAGCAGATGACCCTGAGTCCACTAAA AGAAGTGCACTTTGTATTTTCAAGATTGCCAGGAAACTGCTGTTCCTATTCAGAATCAAAGAGGATAATG AGAGATTTGAGAAGTTGTGTCGCAGCATCCTGAGCAGCATGGATGCTGAGAATGAGCCTAAGGTGTGGTA TGTGTCCCTGGCTTGTTCTAAGGACCTCACCCTCCTTTGGATTCAACAGATCAAGAACATTTTGTGGTAC TGCTGTGATTTTCTCAAGCAGCTCAAGCCTGAAATCCTGCAGGACTCCCGACTCATCACCCTGTACCTCA CGATGCTTGTCACCTTCACAGACACTTCAACGTGGAAAATTCTTCGGGGAAAAGGTGAAAGTCTTCGACC AGCGATGAACCACATTTGTGCAAATATAATGGGACATCTCAACCAGCATGGATTTTATTCTGTGCTGCAG ATATTGTTAACCCGTGGCCTGGCAAGACCCCGTCCTTGTCTATCCAAAGGCACTTTAACAGCAGCTTTTT CTCTAGCGTTACGCCCTGTGATTGCTGCACAGTTCTCAGACAATCTGATTCGGCCGTTCCTCATCCACAT CATGTCTGTGCCTGCTCTGGTGACTCATCTCAGCACAGTGACCCCTGAGCGCCTCACTGTTTTAGAATCC CATGACATGCTTCGTAAATTCATCATATTTTTAAGAGACCAAGATCGATGCCGTGATGTATGTGAAAGTT TAGAAGGATGCCATACGCTTTGTCTAATGGGCAACCTCCTACACTTGGGCTCCCTCAGCCCCAGAGTGTT AGAGGAGGAGACAGATGGGTTCGTGAGTTTGCTCACCCAGACGCTGTGCTACTGTCAGAAGTATGTGTCT CAGAAGAAGTCCAACCTGACCCACTGGCATCCTGTCCTTGGCTGGTTCTCCCAATCTGTGGATTATGGCC TTAACGAGTCAATGCACTTGATCACCAAACAGCTGCAGTTCTTGTGGGGGGTGCCTCTGATCCGGATCTT CTTCTGTGACATCCTGAGCAAGAAGCTACTGGAGAGCCAGGAGCCAGCCCACGCACAGCCAGCATCCCCT CAGAATGTGCTCCCAGTGAAGAGTCTCCTAAAGCGTGCTTTTCAAAAGTCGGCATCAGTCCGGAATATTC TCAGGCCTGTCGGGGGTAAACGGGTCGACTCTGCAGAAGTCCAGAAGGTTTGCAACATCTGTGTCCTCTA CCAGACCTCGCTGACAACTCTCACACAGATTCGGCTGCAGATACTCACAGGTCTCACTTACCTTGATGAC CTGCTTCCCAAACTGTGGGCATTTATCTGTGAGCTCGGGCCCCACGGAGGGTTAAAGCTCTTCTTGGAAT GCCTGAACAATGACACTGAAGAGTCCAAGCAACTCTTGGCCATGCTGATGCTGTTCTGTGACTGTTCGCG GCACCTCATCACAATCCTTGATGACATTGAAGTTTATGAAGAACAGATTTCATTCAAACTGGAAGAGCTG GTCACTATCTCCTCTTTCCTGAATTCTTTTGTGTTTAAGATGATCTGGGATGGAATTGTAGAGAACGCCA AGGGTGAGACCTTGGAGCTGTTCCAGTCTGTCCACGGGTGGCTTATGGTGCTGTACGAGCGGGACTGCCG GCGGCGCTTCACCCCCGAGGACCACTGGCTGCGAAAGGATCTCAAACCTAGCGTGCTCTTCCAAGAACTC GACAGGGACAGAAAACGGGCACAGTTGATCCTGCAGTACATCCCACATGTCATCCCTCACAAAAACAGAG TTCTACTGTTTCGAACCATGGTTACCAAGGAGAAGGAGAAACTGGGGCTGGTGGAAACCAGCTCTGCCTC CCCGCATGTCACTCACATCACCATCCGCCGGTCCAGGATGCTGGAGGACGGCTACGAGCAGCTTAGGCAG CTCTCCCAGCACGCCATGAAGGGGGTCATCCGTGTGAAGTTTGTCAATGACCTCGGGGTGGACGAAGCAG GGATTGATCAAGACGGTGTTTTTAAGGAGTTCTTGGAAGAGATCATCAAGAGAGTTTTTGACCCAGCACT CAATCTGTTCAAGACAACCAGTGGGGATGAGAGGCTGTACCCCTCACCCACATCCTACATCCATGAGAAT TACCTGCAGCTCTTCGAGTTTGTGGGGAAGATGCTGGGGAAGGCTGTGTATGAGGGAATTGTGGTGGACG TGCCATTTGCATCCTTCTTCCTGAGCCAACTGCTTGGGCACCACCACAGCGTCTTCTATAGCTCGGTGGA TGAACTGCCTTCTCTGGACTCCGAGTTCTATAAAAACCTCACCTCCATCAAGCGCTATGATGGGGACATC ACTGACCTGGGCCTGACGCTGTCTTACGACGAGGACGTCATGGGTCAGCTTGTTTGCCATGAACTGATTC CTGGAGGGAAGACCATTCCTGTTACAAATGAAAATAAAATTAGCTACATCCATCTGATGGCACATTTTCG AATGCACACTCAAATAAAAAACCAAACAGCTGCCCTCATTAGCGGATTCCGTTCCATTATCAAACCCGAG TGGATCCGAATGTTCTCAACTCCTGAACTGCAGCGTCTCATCTCTGGCGACAATGCTGAGATTGATCTGG AAGATTTAAAGAAGCACACAGTCTACTACGGTGGTTTCCATGGAAGTCACAGAGTCATCATCTGGCTCTG GGATATTCTGGCCTCCGACTTCACACCGGATGAGAGAGCTATGTTTCTGAAGTTCGTGACCAGCTGCTCC AGACCCCCGCTCCTGGGATTCGCCTACCTCAAGCCTCCATTCTCCATCCGCTGCGTGGAGGTGTCGGACG ATCAGGACACCGGGGACACTCTGGGCAGCGTCCTCCGGGGCTTCTTCACCATCCGCAAGCGGGAGCCAGG CGGCCGCCTGCCCACCTCCTCCACCTGCTTCAACCTGCTCAAGCTGCCCAACTACAGCAAGAAGAGCGTC CTCCGCGAGAAGCTGCGCTACGCCATCAGCATGAACACGGGCTTTGAACTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219024 representing NM_130466
Red=Cloning site Green=Tags(s) MFTLSQTSRAWFIDRARQAREERLVQKERERAAVVIQAHVRSFLCRSRLQRDIRREIDDFFKADDPESTK RSALCIFKIARKLLFLFRIKEDNERFEKLCRSILSSMDAENEPKVWYVSLACSKDLTLLWIQQIKNILWY CCDFLKQLKPEILQDSRLITLYLTMLVTFTDTSTWKILRGKGESLRPAMNHICANIMGHLNQHGFYSVLQ ILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQFSDNLIRPFLIHIMSVPALVTHLSTVTPERLTVLES HDMLRKFIIFLRDQDRCRDVCESLEGCHTLCLMGNLLHLGSLSPRVLEEETDGFVSLLTQTLCYCQKYVS QKKSNLTHWHPVLGWFSQSVDYGLNESMHLITKQLQFLWGVPLIRIFFCDILSKKLLESQEPAHAQPASP QNVLPVKSLLKRAFQKSASVRNILRPVGGKRVDSAEVQKVCNICVLYQTSLTTLTQIRLQILTGLTYLDD LLPKLWAFICELGPHGGLKLFLECLNNDTEESKQLLAMLMLFCDCSRHLITILDDIEVYEEQISFKLEEL VTISSFLNSFVFKMIWDGIVENAKGETLELFQSVHGWLMVLYERDCRRRFTPEDHWLRKDLKPSVLFQEL DRDRKRAQLILQYIPHVIPHKNRVLLFRTMVTKEKEKLGLVETSSASPHVTHITIRRSRMLEDGYEQLRQ LSQHAMKGVIRVKFVNDLGVDEAGIDQDGVFKEFLEEIIKRVFDPALNLFKTTSGDERLYPSPTSYIHEN YLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVDELPSLDSEFYKNLTSIKRYDGDI TDLGLTLSYDEDVMGQLVCHELIPGGKTIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPE WIRMFSTPELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPDERAMFLKFVTSCS RPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRGFFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSV LREKLRYAISMNTGFELS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130466 |
| ORF Size | 3204 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130466.4 |
| RefSeq Size | 5753 bp |
| RefSeq ORF | 3207 bp |
| Locus ID | 89910 |
| UniProt ID | Q7Z3V4 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 122.9 kDa |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219024L1 | Lenti-ORF clone of UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 9,840.00 |
|
| RC219024L2 | Lenti-ORF clone of UBE3B (mGFP-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 10,260.00 |
|
| RC219024L3 | Lenti-ORF clone of UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 10,260.00 |
|
| RC219024L4 | Lenti-ORF clone of UBE3B (mGFP-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 10,260.00 |
|
| RG219024 | UBE3B (tGFP-tagged) - Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 9,040.00 |
|
| SC315135 | UBE3B (untagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 1 |
CNY 10,450.00 |
