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CSB (ERCC6) (NM_000124) Human Tagged ORF Clone
CAT＃: RC219020
ERCC6 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6)
ORF Plasmid: tGFP
"NM_000124" in other vectors (6)
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CNY 15,320.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219020 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTCA GTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTGTCCTT TCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGGCCA GCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGG GTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCAT CCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCTGCCACCA GCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAGCTAAAAAA GATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAACTAGATCAC GCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGA CTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGA GAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCCCCAGTCACGC CTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCCAAAAAAGAGGAGCG TTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAAGTGGGATTGCCAAAG GCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGT ATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGG AGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGAGAAGGAGGAG GAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTATTATAAGCAGCGGTTAAGGAG ATGGAATAAACTGAGACTGCAGGACAAAGAGAAACGTCTGAAGCTGGAGGACGATTCTGAGGAAAGTGAT GCTGAATTTGACGAAGGTTTTAAAGTGCCAGGTTTTCTGTTCAAAAAGCTTTTTAAGTACCAGCAGACAG GTGTTAGGTGGCTGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGATGAAATGGGATTGGG CAAGACCATCCAGATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGTGGTTCAAAT TACAGGTTTGAGGGGTTGGGTCCAACTGTAATTGTCTGTCCAACAACAGTGATGCATCAGTGGGTGAAGG AATTTCACACGTGGTGGCCTCCGTTCAGAGTGGCAATTCTACATGAAACCGGTTCCTATACCCACAAAAA GGAGAAACTAATTCGAGATGTTGCTCATTGTCATGGAATTTTGATCACATCTTACTCCTACATTCGATTG ATGCAGGATGACATTAGCAGGTATGACTGGCACTATGTGATCTTGGACGAAGGACACAAAATTCGAAATC CAAATGCTGCTGTCACCCTTGCTTGCAAACAGTTTTGCACCCCTCATCGGATCATTCTGTCTGGCTCACC GATGCAAAATAACCTCCGAGAGCTGTGGTCGCTCTTTGACTTCATCTTCCCGGGAAAGTTAGGCACGTTG CCTGTGTTTATGGAGCAGTTCTCCGTCCCCATCACCATGGGGGGATATTCAAATGCTTCCCCAGTACAGG TCAAAACTGCTTACAAGTGTGCATGTGTCTTACGAGATACCATAAATCCATACCTACTGCGGAGAATGAA GTCAGATGTCAAGATGAGCCTTTCTTTGCCAGATAAAAATGAACAGGTCTTATTTTGCCGTCTTACAGAT GAGCAGCATAAAGTCTACCAAAATTTCGTTGATTCCAAAGAAGTTTACAGGATTCTCAATGGAGAGATGC AGATTTTCTCCGGACTTATAGCCCTAAGAAAAATTTGCAACCACCCTGATCTCTTTTCTGGAGGTCCCAA GAATCTCAAAGGTCTTCCTGATGATGAACTAGAAGAAGATCAGTTTGGGTACTGGAAACGTTCTGGGAAA ATGATTGTTGTTGAGTCTTTGTTGAAAATATGGCACAAGCAGGGTCAGCGAGTATTGCTGTTTTCTCAGT CAAGGCAGATGCTGGACATACTTGAAGTATTCCTTAGAGCCCAAAAGTATACCTATCTCAAGATGGATGG TACCACTACAATAGCTTCAAGACAGCCACTGATTACGAGATACAATGAGGACACATCCATATTTGTGTTT CTTCTGACCACGCGGGTGGGTGGCTTAGGTGTCAACCTGACGGGGGCAAACAGAGTTGTCATCTATGACC CAGACTGGAACCCAAGCACGGACACGCAGGCCCGGGAGCGAGCATGGAGAATAGGCCAGAAGAAGCAAGT GACTGTGTACAGGCTCCTGACTGCGGGCACCATTGAAGAAAAGATCTACCACCGACAAATCTTCAAGCAG TTTTTGACAAATAGAGTGCTAAAAGACCCAAAACAAAGGCGGTTTTTCAAATCCAATGATCTCTATGAGC TATTTACTCTGACTAGTCCTGATGCATCCCAGAGCACTGAAACAAGTGCAATTTTTGCAGGAACTGGATC AGATGTTCAGACACCCAAATGCCATCTAAAAAGAAGGATTCCACCAGCCTTTGGAGCAGACCATGATGTT CCAAAACGCAAGAAGTTCCCTGCTTCTAACATATCTGTAAATGATGCCACATCATCTGAAGAGAAATCTG AGGCTAAAGGAGCTGAAGTAAATGCAGTAACTTCTAATCGAAGTGATCCTTTGAAAGATGACCCTCACAT GAGTAGTAATGTAACTAGCAATGATAGGCTTGGAGAAGAGACAAATGCAGTATCTGGACCAGAAGAGTTG TCAGTGATTAGTGGAAATGGGGAATGTTCAAATTCTTCAGGAACAGGCAAAACTTCTATGCCATCTGGTG ATGAAAGCATTGATGAAAAGTTAGGTCTTTCTTACAAAAGAGAAAGACCCAGCCAGGCTCAAACAGAAGC TTTTTGGGAGAATAAACAAATGGAAAATAATTTTTATAAGCACAAGTCAAAAACAAAACATCATAGTGTG GCAGAAGAAGAGACCCTGGAGAAACATCTGAGACCAAAGCAAAAGCCTAAGAACTCTAAGCATTGCAGAG ACGCCAAGTTTGAAGGAACTCGAATTCCACACCTGGTGAAGAAAAGGCGTTACCAGAAGCAAGACAGTGA AAACAAGAGTGAGGCCAAGGAACAGAGCAATGACGATTATGTTTTGGAAAAGCTTTTCAAAAAATCAGTT GGCGTGCACAGTGTCATGAAGCACGATGCCATCATGGATGGAGCCAGCCCAGATTATGTACTGGTGGAGG CAGAAGCCAACCGAGTGGCCCAGGATGCCCCGAAAGCACTGAGGCTCTCTCGTCAGCGGTGTCTGGGAGC AGTGTCTGGTGTTCCCACCTGGACTGGCCACAGGGGGATTTCTGGTGCACCAGCAGGAAAAAAGAGTAGA TTTGGTAAGAAAAGGAATTCTAACTTCTCTGTGCAGCATCCTTCATCAACATCTCCAACAGAGAAGTGCC AGGATGGCATCATGAAAAAGGAGGGAAAAGATAATGTCCCTGAGCATTTTAGTGGAAGAGCAGAAGATGC AGACTCTTCATCCGGGCCCCTCGCTTCCTCCTCACTCTTGGCTAAAATGAGAGCTAGAAACCACCTGATT CTGCCAGAGCGTTTAGAAAGTGAAAGCGGGCACCTGCAGGAAGCTTCTGCCCTGCTGCCCACCACAGAAC ACGATGACCTTCTGGTGGAGATGAGAAACTTCATCGCTTTCCAGGCCCACACTGATGGCCAGGCCAGCAC CAGGGAGATACTGCAGGAGTTTGAATCCAAGTTATCTGCATCACAGTCTTGTGTCTTCCGAGAACTATTG AGAAATCTGTGCACTTTCCATAGAACTTCTGGTGGTGAAGGAATTTGGAAACTCAAGCCAGAATACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219020 protein sequence
Red=Cloning site Green=Tags(s) MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVGCASAAPRRGP ALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDL TSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDH ASLEEDAEPGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAK LSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPK ARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQ EIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLRRWNKLRLQDKEKRLKLEDDSEESD AEFDEGFKVPGFLFKKLFKYQQTGVRWLWELHCQQAGGILGDEMGLGKTIQIIAFLAGLSYSKIRTRGSN YRFEGLGPTVIVCPTTVMHQWVKEFHTWWPPFRVAILHETGSYTHKKEKLIRDVAHCHGILITSYSYIRL MQDDISRYDWHYVILDEGHKIRNPNAAVTLACKQFCTPHRIILSGSPMQNNLRELWSLFDFIFPGKLGTL PVFMEQFSVPITMGGYSNASPVQVKTAYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTD EQHKVYQNFVDSKEVYRILNGEMQIFSGLIALRKICNHPDLFSGGPKNLKGLPDDELEEDQFGYWKRSGK MIVVESLLKIWHKQGQRVLLFSQSRQMLDILEVFLRAQKYTYLKMDGTTTIASRQPLITRYNEDTSIFVF LLTTRVGGLGVNLTGANRVVIYDPDWNPSTDTQARERAWRIGQKKQVTVYRLLTAGTIEEKIYHRQIFKQ FLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQSTETSAIFAGTGSDVQTPKCHLKRRIPPAFGADHDV PKRKKFPASNISVNDATSSEEKSEAKGAEVNAVTSNRSDPLKDDPHMSSNVTSNDRLGEETNAVSGPEEL SVISGNGECSNSSGTGKTSMPSGDESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSV AEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSV GVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSR FGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLI LPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELL RNLCTFHRTSGGEGIWKLKPEYC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000124 |
| ORF Size | 4479 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000124.4 |
| RefSeq Size | 8993 bp |
| RefSeq ORF | 4482 bp |
| Locus ID | 2074 |
| UniProt ID | Q03468 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Nucleotide excision repair |
| MW | 168.3 kDa |
| Gene Summary | This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence
,Crochemore, C;Fernández-Molina, C;Montagne, B;Salles, A;Ricchetti, M;,
Nat Commun
,PubMed ID 31811121
[CSB]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219020L1 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6), Myc-DDK-tagged |
CNY 17,720.00 |
|
| RC219020L2 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6), mGFP tagged |
CNY 16,820.00 |
|
| RC219020L3 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6), Myc-DDK-tagged |
CNY 16,820.00 |
|
| RC219020L4 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6), mGFP tagged |
CNY 17,720.00 |
|
| RG219020 | ERCC6 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6) |
CNY 16,920.00 |
|
| SC309065 | ERCC6 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6) |
CNY 10,450.00 |
