CD47 (NM_001777) Human Tagged ORF Clone
CAT#: RC218813
CD47 (Myc-DDK-tagged)-Human CD47 molecule (CD47), transcript variant 1
ORF Plasmid: tGFP
"NM_001777" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | IAP; MER6; OA3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218813 representing NM_001777
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCCCCTGGTAGCGGCGCTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATA AAACAAAATCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATGGA GGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTACACCTTTGATGGA GCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATTGAAGTCTCACAATTACTAAAAG GAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTGAAGTAAC AGAATTAACCAGAGAAGGTGAAACGATCATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCAAAT GAAAATATTCTTATTGTTATTTTCCCAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAA CACTTAAATATAGATCCGGTGGTATGGATGAGAAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCAC TGTCATTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGTGAATATTCATTAAAGAATGCTACTGGCCTT GGTTTAATTGTGACTTCTACAGGGATATTAATATTACTTCACTACTATGTGTTTAGTACAGCGATTGGAT TAACCTCCTTCGTCATTGCCATATTGGTTATTCAGGTGATAGCCTATATCCTCGCTGTGGTTGGACTGAG TCTCTGTATTGCGGCGTGTATACCAATGCATGGCCCTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTA GCACAATTACTTGGACTAGTTTATATGAAATTTGTGGCTTCCAATCAGAAGACTATACAACCTCCTAGGA AAGCTGTAGAGGAACCCCTTAATGCATTCAAAGAATCAAAAGGAATGATGAATGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218813 representing NM_001777
Red=Cloning site Green=Tags(s) MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDG ALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPN ENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGL GLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILAL AQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001777 |
ORF Size | 969 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001777.4 |
RefSeq Size | 5346 bp |
RefSeq ORF | 972 bp |
Locus ID | 961 |
UniProt ID | Q08722 |
Domains | CD47 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | ECM-receptor interaction |
MW | 35.21 kDa |
Gene Summary | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
,null,
The Journal of Biological Chemistry
,PubMed ID 36796515
[CD47]
|
Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation
,Yang, Z;Shi, J;Xie, J;Wang, Y;Sun, J;Liu, T;Zhao, Y;Zhao, X;Wang, X;Ma, Y;Malkoc, V;Chiang, C;Deng, W;Chen, Y;Fu, Y;Kwak, KJ;Fan, Y;Kang, C;Yin, C;Rhee, J;Bertani, P;Otero, J;Lu, W;Yun, K;Lee, AS;Jiang, W;Teng, L;Kim, BYS;Lee, LJ;,
Nat Biomed Eng
,PubMed ID 31844155
[CD47]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218813L1 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC218813L2 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RC218813L3 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC218813L4 | Lenti ORF clone of Human CD47 molecule (CD47), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RG218813 | CD47 (tGFP-tagged) - Human CD47 molecule (CD47), transcript variant 1 |
CNY 4,000.00 |
|
SC119049 | CD47 (untagged)-Human CD47 molecule (CD47), transcript variant 1 |
CNY 3,600.00 |