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IQGAP1 (NM_003870) Human Tagged ORF Clone
CAT＃: RC218549
IQGAP1 (Myc-DDK-tagged)-Human IQ motif containing GTPase activating protein 1 (IQGAP1)
ORF Plasmid: tGFP
"NM_003870" in other vectors (6)
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CNY 11,336.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HUMORFA01; p195; SAR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218549 representing NM_003870
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGCCGCAGACGAGGTTGACGGGCTGGGCGTGGCCCGGCCGCACTATGGCTCTGTCCTGGATAATG AAAGACTTACTGCAGAGGAGATGGATGAAAGGAGACGTCAGAACGTGGCTTATGAGTACCTTTGTCATTT GGAAGAAGCGAAGAGGTGGATGGAAGCATGCCTAGGGGAAGATCTGCCTCCCACCACAGAACTGGAGGAG GGGCTTAGGAATGGGGTCTACCTTGCCAAACTGGGGAACTTCTTCTCTCCCAAAGTAGTGTCCCTGAAAA AAATCTATGATCGAGAACAGACCAGATACAAGGCGACTGGCCTCCACTTTAGACACACTGATAATGTGAT TCAGTGGTTGAATGCCATGGATGAGATTGGATTGCCTAAGATTTTTTACCCAGAAACTACAGATATCTAT GATCGAAAGAACATGCCAAGATGTATCTACTGTATCCATGCACTCAGTTTGTACCTGTTCAAGCTAGGCC TGGCCCCTCAGATTCAAGACCTATATGGAAAGGTTGACTTCACAGAAGAAGAAATCAACAACATGAAGAC TGAGTTGGAGAAGTATGGCATCCAGATGCCTGCCTTTAGCAAGATTGGGGGCATCTTGGCTAATGAACTG TCAGTGGATGAAGCCGCATTACATGCTGCTGTTATTGCTATTAATGAAGCTATTGACCGTAGAATTCCAG CCGACACATTTGCAGCTTTGAAAAATCCGAATGCCATGCTTGTAAATCTTGAAGAGCCCTTGGCATCCAC TTACCAGGATATACTTTACCAGGCTAAGCAGGACAAAATGACAAATGCTAAAAACAGGACAGAAAACTCA GAGAGAGAAAGAGATGTTTATGAGGAGCTGCTCACGCAAGCTGAAATTCAAGGCAATATAAACAAAGTCA ATACATTTTCTGCATTAGCAAATATCGACCTGGCTTTAGAACAAGGAGATGCACTGGCCTTGTTCAGGGC TCTGCAGTCACCAGCCCTGGGGCTTCGAGGACTGCAGCAACAGAATAGCGACTGGTACTTGAAGCAGCTC CTGAGTGATAAACAGCAGAAGAGACAGAGTGGTCAGACTGACCCCCTGCAGAAGGAGGAGCTGCAGTCTG GAGTGGATGCTGCAAACAGTGCTGCCCAGCAATATCAGAGAAGATTGGCAGCAGTAGCACTGATTAATGC TGCAATCCAGAAGGGTGTTGCTGAGAAGACTGTTTTGGAACTGATGAATCCCGAAGCCCAGCTGCCCCAG GTGTATCCATTTGCCGCCGATCTCTATCAGAAGGAGCTGGCTACCCTGCAGCGACAAAGTCCTGAACATA ATCTCACCCACCCAGAGCTCTCTGTCGCAGTGGAGATGTTGTCATCGGTGGCCCTGATCAACAGGGCATT GGAATCAGGAGATGTGAATACAGTGTGGAAGCAATTGAGCAGTTCAGTTACTGGTCTTACCAATATTGAG GAAGAAAACTGTCAGAGGTATCTCGATGAGTTGATGAAACTGAAGGCTCAGGCACATGCAGAGAATAATG AATTCATTACATGGAATGATATCCAAGCTTGCGTGGACCATGTGAACCTGGTGGTGCAAGAGGAACATGA GAGGATTTTAGCCATTGGTTTAATTAATGAAGCCCTGGATGAAGGTGATGCCCAAAAGACTCTGCAGGCC CTACAGATTCCTGCAGCTAAACTTGAGGGAGTCCTTGCAGAAGTGGCCCAGCATTACCAAGACACGCTGA TTAGAGCGAAGAGAGAGAAAGCCCAGGAAATCCAGGATGAGTCAGCTGTGTTATGGTTGGATGAAATTCA AGGTGGAATCTGGCAGTCCAACAAAGACACCCAAGAAGCACAGAAGTTTGCCTTAGGAATCTTTGCCATT AATGAGGCAGTAGAAAGTGGTGATGTTGGCAAAACACTGAGTGCCCTTCGCTCCCCTGATGTTGGCTTGT ATGGAGTCATCCCTGAGTGTGGTGAAACTTACCACAGTGATCTTGCTGAAGCCAAGAAGAAAAAACTGGC AGTAGGAGATAATAACAGCAAGTGGGTGAAGCACTGGGTAAAAGGTGGATATTATTATTACCACAATCTG GAGACCCAGGAAGGAGGATGGGATGAACCCCCAAATTTTGTGCAAAATTCTATGCAGCTTTCTCGGGAGG AGATCCAGAGTTCTATCTCTGGGGTGACTGCCGCATATAACCGAGAACAGCTGTGGCTGGCCAATGAAGG CCTGATCACCAGGCTGCAGGCTCGCTGCCGTGGATACTTAGTTCGACAGGAATTCCGATCCAGGATGAAT TTCCTGAAGAAACAAATCCCTGCCATCACCTGCATTCAGTCACAGTGGAGAGGATACAAGCAGAAGAAGG CATATCAAGATCGGTTAGCTTACCTGCGCTCCCACAAAGATGAAGTTGTAAAGATTCAGTCCCTGGCAAG GATGCACCAAGCTCGAAAGCGCTATCGAGATCGCCTGCAGTACTTCCGGGACCATATAAATGACATTATC AAAATCCAGGCTTTTATTCGGGCAAACAAAGCTCGGGATGACTACAAGACTCTCATCAATGCTGAGGATC CTCCTATGGTTGTGGTCCGAAAATTTGTCCACCTGCTGGACCAAAGTGACCAGGATTTTCAGGAGGAGCT TGACCTTATGAAGATGCGGGAAGAGGTTATCACCCTCATTCGTTCTAACCAGCAGCTGGAGAATGACCTC AATCTCATGGATATCAAAATTGGACTGCTAGTGAAAAATAAGATTACGTTGCAGGATGTGGTTTCCCACA GTAAAAAACTTACCAAAAAAAATAAGGAACAGTTGTCTGATATGATGATGATAAATAAACAGAAGGGAGG TCTCAAGGCTTTGAGCAAGGAGAAGAGAGAGAAGTTGGAAGCTTACCAGCACCTGTTTTATTTATTGCAA ACCAATCCCACCTATCTGGCCAAGCTCATTTTTCAGATGCCCCAGAACAAGTCCACCAAGTTCATGGACT CTGTAATCTTCACACTCTACAACTACGCGTCCAACCAGCGAGAGGAGTACCTGCTCCTGCGGCTCTTTAA GACAGCACTCCAAGAGGAAATCAAGTCGAAGGTAGATCAGATTCAAGAGATTGTGACAGGAAATCCTACG GTTATTAAAATGGTTGTAAGTTTCAACCGTGGTGCCCGTGGCCAGAATGCCCTGAGACAGATCTTGGCCC CAGTCGTGAAGGAAATTATGGATGACAAATCTCTCAACATCAAAACTGACCCTGTGGATATTTACAAATC TTGGGTTAATCAGATGGAGTCTCAGACAGGAGAGGCAAGCAAACTGCCCTATGATGTGACCCCTGAGCAG GCGCTAGCTCATGAAGAAGTGAAGACACGGCTAGACAGCTCCATCAGGAACATGCGGGCTGTGACAGACA AGTTTCTCTCAGCCATTGTCAGCTCTGTGGACAAAATCCCTTATGGGATGCGCTTCATTGCCAAAGTGCT GAAGGACTCGTTGCATGAGAAGTTCCCTGATGCTGGTGAGGATGAGCTGCTGAAGATTATTGGTAACTTG CTTTATTATCGATACATGAATCCAGCCATTGTTGCTCCTGATGCCTTTGACATCATTGACCTGTCAGCAG GAGGCCAGCTTACCACAGACCAACGCCGAAATCTGGGCTCCATTGCAAAAATGCTTCAGCATGCTGCTTC CAATAAGATGTTTCTGGGAGATAATGCCCACTTAAGCATCATTAATGAATATCTTTCCCAGTCCTACCAG AAATTCAGACGGTTTTTCCAAACTGCTTGTGATGTCCCAGAGCTTCAGGATAAATTTAATGTGGATGAGT ACTCTGATTTAGTAACCCTCACCAAACCAGTAATCTACATTTCCATTGGTGAAATCATCAACACCCACAC TCTCCTGTTGGATCACCAGGATGCCATTGCTCCGGAGCACAATGATCCAATCCACGAACTGCTGGACGAC CTCGGCGAGGTGCCCACCATCGAGTCCCTGATAGGGGAAAGCTCTGGCAATTTAAATGACCCAAATAAGG AGGCACTGGCTAAGACGGAAGTGTCTCTCACCCTGACCAACAAGTTCGACGTGCCTGGAGATGAGAATGC AGAAATGGATGCTCGAACCATCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCA GGAGAGACCTTGACTGAAATCCTAGAAACACCAGCCACCAGTGAACAGGAAGCAGAACATCAGAGAGCCA TGCAGAGACGTGCTATCCGTGATGCCAAAACACCTGACAAGATGAAAAAGTCAAAATCTGTAAAGGAAGA CAGCAACCTCACTCTTCAAGAGAAGAAAGAGAAGATCCAGACAGGTTTAAAGAAGCTAACAGAGCTTGGA ACCGTGGACCCAAAGAACAAATACCAGGAACTGATCAACGACATTGCCAGGGATATTCGGAATCAGCGGA GGTACCGACAGAGGAGAAAGGCCGAACTAGTGAAACTGCAACAGACATACGCTGCTCTGAACTCTAAGGC CACCTTTTATGGGGAGCAGGTGGATTACTATAAAAGCTATATCAAAACCTGCTTGGATAACTTAGCCAGC AAGGGCAAAGTCTCCAAAAAGCCTAGGGAAATGAAAGGAAAGAAAAGCAAAAAGATTTCTCTGAAATATA CAGCAGCAAGACTACATGAAAAAGGAGTTCTTCTGGAAATTGAGGACCTGCAAGTGAATCAGTTTAAAAA TGTTATATTTGAAATCAGTCCAACAGAAGAAGTTGGAGACTTCGAAGTGAAAGCCAAATTCATGGGAGTT CAAATGGAGACTTTTATGTTACATTATCAGGACCTGCTGCAGCTACAGTATGAAGGAGTTGCAGTCATGA AATTATTTGATAGAGCTAAAGTAAATGTCAACCTCCTGATCTTCCTTCTCAACAAAAAGTTCTACGGGAA G AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC218549 representing NM_003870
Red=Cloning site Green=Tags(s) MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEE GLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIY DRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKTELEKYGIQMPAFSKIGGILANEL SVDEAALHAAVIAINEAIDRRIPADTFAALKNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENS ERERDVYEELLTQAEIQGNINKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQL LSDKQQKRQSGQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQ VYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLSSSVTGLTNIE EENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQA LQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAI NEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNL ETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMN FLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDII KIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDL NLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQ TNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPT VIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQ ALAHEEVKTRLDSSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNL LYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQ KFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDD LGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQP GETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQTGLKKLTELG TVDPKNKYQELINDIARDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLAS KGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGV QMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003870 |
| ORF Size | 4971 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003870.4 |
| RefSeq Size | 7219 bp |
| RefSeq ORF | 4974 bp |
| Locus ID | 8826 |
| UniProt ID | P46940 |
| Domains | IQ, RasGAP_C, WW, CH, RasGAP |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Regulation of actin cytoskeleton |
| MW | 189.1 kDa |
| Gene Summary | This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Reactivation of epigenetically silenced miR-124 reverses the epithelial-to-mesenchymal transition and inhibits invasion in endometrial cancer cells via the direct repression of IQGAP1 expression
,Dong, P;Ihira, K;Xiong, Y;Watari, H;Hanley, SJ;Yamada, T;Hosaka, M;Kudo, M;Yue, J;Sakuragi, N;,
Oncotarget
,PubMed ID 26934121
[IQGAP1]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218549L1 | Lenti ORF clone of Human IQ motif containing GTPase activating protein 1 (IQGAP1), Myc-DDK-tagged |
CNY 18,430.00 |
|
| RC218549L2 | Lenti ORF clone of Human IQ motif containing GTPase activating protein 1 (IQGAP1), mGFP tagged |
CNY 18,430.00 |
|
| RC218549L3 | Lenti ORF clone of Human IQ motif containing GTPase activating protein 1 (IQGAP1), Myc-DDK-tagged |
CNY 18,430.00 |
|
| RC218549L4 | Lenti ORF clone of Human IQ motif containing GTPase activating protein 1 (IQGAP1), mGFP tagged |
CNY 13,736.00 |
|
| RG218549 | IQGAP1 (tGFP-tagged) - Human IQ motif containing GTPase activating protein 1 (IQGAP1) |
CNY 18,150.00 |
|
| SC117725 | IQGAP1 (untagged)-Human IQ motif containing GTPase activating protein 1 (IQGAP1) |
CNY 11,344.00 |
