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IQSEC2 (NM_015075) Human Tagged ORF Clone
CAT＃: RC218367
- TrueORF®
IQSEC2 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 2
ORF Plasmid: tGFP
"NM_015075" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BRAG1; IQ-ArfGEF; MRX1; MRX18; MRX78 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218367 representing NM_015075
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCCCGGGAGGAGCAGCAGGTCCACAGCGTCTCATACTCTACACCAGTATTGCTGTCCTACTC AGGTCCTTGACTCCATGAAGCTTACCCCCTCAGGCAGGCTGGCAGAGAGCAGTGTGGAGGGTGATGCCCC AGGCAGTGACCTGAGCACAGCGGTTGATAGTCCTGGGAGCCAACCCCCCTACCGGCTGAGCCAGCTGCCC CCCTCCAGCAGCCACATGGGGGGCCCCCCTGCTGGAGTGGGCCTTCCCTGGGCTCAGCGGGCACGCCTCC AGCCAGCCAGTGTCGCCCTGAGGAAGCAGGAGGAGGAGGAGATAAAGCGCTCCAAGGCCCTATCGGACAG CTATGAACTCTCCACAGACCTGCAGGACAAGAAGGTGGAAATGCTGGAGAGGAAGTATGGGGGCTCCTTC CTGAGCCGCAGGGCTGCCAGGACCATCCAGACAGCCTTCCGCCAGTACCGCATGAACAAGAACTTTGAGC GGCTACGCAGCTCAGCCTCAGAGAGCCGCATGTCCCGCCGCATCATCCTTTCCAACATGCGGATGCAGTT CTCCTTTGAGGAGTATGAGAAGGCACAGAACCCCGCGTACTTCGAGGGCAAGCCTGCCTCGCTGGACGAG GGTGCCATGGCTGGTGCCCGGAGCCACCGGCTTGAACGGGGGCTCCCATATGGAGGCTCCTGTGGTGGGG GCATCGATGGTGGTGGAAGCTCCGTCACCACATCTGGAGAGTTTTCTAATGACATCACAGAACTTGAGGA CTCCTTCTCCAAACAGGTAAAGTCTCTGGCTGAATCCATCGACGAAGCCCTGAACTGCCACCCGTCAGGG CCCATGTCTGAGGAGCCAGGGTCAGCCCAGCTGGAGAAGCGGGAGTCAAAGGAACAGCAAGAGGACAGCT CAGCCACATCCTTCAGTGATCTTCCCCTCTACCTGGATGACACAGTTCCCCAACAATCCCCTGAGCGACT GCCCAGCACAGAACCCCCACCCCAGGGCCGGCCCGAGTTCTGGGCGCCAGCCCCTCTCCCGCCAGTTCCT CCACCAGTGCCATCAGGAACCCGGGAAGACGGTAGCCGTGAGGAAGGCACTCGCAGGGGTCCCGGGTGCT TGGAGTGCCGGGATTTCCGGCTGCGGGCTGCCCACCTTCCCCTGCTTACCATTGAGCCTCCTAGTGACAG CTCCGTGGACCTGAGTGACCGCTCAGATCGCGGCTCTGTCCACCGCCAGCTGGTGTATGAGGCTGATGGC TGCAGCCCCCATGGGACCCTGAAGCACAAGGGGCCACCAGGCAGGGCCCCGATCCCACACCGCCACTACC CAGCCCCTGAAGGCCCAGCCCCAGCCCCACCAGGGCCCCTGCCACCAGCCCCCAACAGTGGCACTGGGCC CAGTGGTGTGGCTGGGGGTCGGAGGTTGGGGAAGTGCGAGGCAGCAGGCGAGAACTCTGATGGTGGAGAT AACGAGAGCCTTGAGAGCTCCAGCAATTCCAATGAGACCATCAACTGCAGCTCCGGCTCCTCTTCTCGGG ACAGTCTAAGGGAGCCTCCGGCTACCGGCCTGTGCAAGCAGACTTACCAGCGGGAGACAAGGCATAGCTG GGACTCGCCAGCTTTCAACAATGATGTGGTCCAGAGGCGGCACTACCGAATCGGCCTCAACCTCTTCAAC AAGAAGCCAGAGAAGGGTATCCAGTATCTGATCGAGCGGGGCTTCCTGTCAGACACACCGGTGGGAGTGG CTCACTTCATCCTGGAGCGGAAAGGCCTCAGCCGGCAGATGATAGGGGAATTCCTAGGGAACCGGCAGAA GCAGTTCAACAGAGACGTGTTGGACTGTGTGGTGGATGAGATGGACTTCTCCTCCATGGATCTGGATGAT GCGCTCCGGAAGTTCCAGTCCCATATCCGGGTTCAGGGTGAGGCCCAGAAAGTGGAGCGACTCATCGAAG CCTTCAGCCAGCGGTACTGTGTCTGTAACCCAGCCCTCGTGCGCCAGTTCCGGAACCCAGACACCATCTT CATCCTTGCTTTTGCCATCATCCTCCTCAATACCGACATGTACAGTCCCAGCGTCAAAGCTGAACGAAAG ATGAAACTAGATGACTTCATCAAGAACCTGAGAGGGGTTGACAATGGTGAAGACATCCCCCGAGACCTCC TGGTGGGCATCTACCAGCGCATCCAGGGGCGTGAACTGCGGACCAACGATGACCATGTGTCCCAGGTGCA GGCTGTGGAGCGCATGATTGTTGGAAAGAAACCAGTCCTGTCTCTCCCTCACCGTCGACTGGTTTGCTGC TGCCAGCTCTACGAGGTGCCAGATCCAAACCGCCCCCAGAGGCTAGGGTTGCATCAGCGGGAGGTCTTCC TCTTCAATGATCTCCTTGTGGTCACCAAAATTTTCCAGAAGAAGAAGATCTTGGTGACGTACAGTTTCCG TCAGTCTTTCCCCCTCGTGGAAATGCACATGCAGCTCTTCCAGAATTCATATTACCAGTTTGGGATCAAG TTGCTGTCTGCAGTACCTGGTGGGGAGCGAAAAGTCCTCATCATCTTCAATGCCCCCAGCCTCCAGGACC GGCTGCGCTTTACATCCGACCTGCGCGAGTCCATTGCGGAGGTGCAGGAGATGGAGAAATACCGTGTGGA GTCGGAGCTGGAGAAGCAGAAAGGTATGATGCGGCCTAACGCCTCACAGCCTGGAGGGGCCAAGGACTCA GTGAATGGGACGATGGCCCGCAGTAGCCTGGAGGACACTTACGGGGCAGGCGATGGGCTCAAACGGGGCG CACTCAGCAGTTCCCTGCGAGACCTCTCTGATGCAGGGGTCTGTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218367 representing NM_015075
Red=Cloning site Green=Tags(s) MEPPGRSSRSTASHTLHQYCCPTQVLDSMKLTPSGRLAESSVEGDAPGSDLSTAVDSPGSQPPYRLSQLP PSSSHMGGPPAGVGLPWAQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERKYGGSF LSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEKAQNPAYFEGKPASLDE GAMAGARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDITELEDSFSKQVKSLAESIDEALNCHPSG PMSEEPGSAQLEKRESKEQQEDSSATSFSDLPLYLDDTVPQQSPERLPSTEPPPQGRPEFWAPAPLPPVP PPVPSGTREDGSREEGTRRGPGCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADG CSPHGTLKHKGPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGGD NESLESSSNSNETINCSSGSSSRDSLREPPATGLCKQTYQRETRHSWDSPAFNNDVVQRRHYRIGLNLFN KKPEKGIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSSMDLDD ALRKFQSHIRVQGEAQKVERLIEAFSQRYCVCNPALVRQFRNPDTIFILAFAIILLNTDMYSPSVKAERK MKLDDFIKNLRGVDNGEDIPRDLLVGIYQRIQGRELRTNDDHVSQVQAVERMIVGKKPVLSLPHRRLVCC CQLYEVPDPNRPQRLGLHQREVFLFNDLLVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIK LLSAVPGGERKVLIIFNAPSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPNASQPGGAKDS VNGTMARSSLEDTYGAGDGLKRGALSSSLRDLSDAGVCY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015075 |
| ORF Size | 2847 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015075.2 |
| RefSeq Size | 5248 bp |
| RefSeq ORF | 2850 bp |
| Locus ID | 23096 |
| UniProt ID | Q5JU85 |
| Protein Pathways | Endocytosis |
| MW | 104.9 kDa |
| Gene Summary | This gene encodes a guanine nucleotide exchange factor for the ARF family of small GTP-binding proteins. The encoded protein is a component of the postsynaptic density at excitatory synapses, and may play a critical role in cytoskeletal and synaptic organization through the activation of selected ARF substrates including ARF1 and ARF6. Mutations in this gene have been implicated in nonsyndromic X-linked cognitive disability. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218367L3 | Lenti-ORF clone of IQSEC2 (Myc-DDK-tagged)-Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 2 |
CNY 9,030.00 |
|
| RC218367L4 | Lenti-ORF clone of IQSEC2 (mGFP-tagged)-Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 2 |
CNY 9,030.00 |
|
| RG218367 | IQSEC2 (tGFP-tagged) - Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 2 |
CNY 7,890.00 |
|
| SC304220 | IQSEC2 (untagged)-Human IQ motif and Sec7 domain 2 (IQSEC2), transcript variant 2 |
CNY 11,128.00 |
