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CERKL (NM_001030313) Human Tagged ORF Clone
CAT＃: RC218175
CERKL (Myc-DDK-tagged)-Human ceramide kinase-like (CERKL), transcript variant 4
ORF Plasmid: tGFP
"NM_001030313" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RP26 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218175 representing NM_001030313
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAGGAGGCGCAGGAACCGGGTGAGTGCCCTGGAGGGCGGCCGGGAGGAAGAGGCGCCCCCGG AGGCTGCCGCTGTGCCTCCGGCGCTGTTAACGTCCCCGCAGCAGACGGAGGCGGCGGCCGAGCGGATTCT GCTCCGGGGCATCTTCGAGATCGGGAGGGACAGTTGTGACGTGGTGCTGAGCGAGCGAGCACTGCGGTGG CGGCCCATTCAGCCCGAGCGCCCGGCGGGTGATTCTAAGTATGACTTGCTATGTAAAGAAGAATTTATTG AACTCAAAGACATATTCTCTGTGAAACTGAAACGGCGTTGTTCTGTTAAACAGCAGAGAAGTGGTACTTT ATTAGGTATCACACTCTTCATCTGCTTGAAAAAGGAACAAAATAAACTAAAGAATTCTACACTTGATCTT ATTAATTTAAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAGAAAATATTGGCAGGCTTTCCAA ACAGACCGAAGTCATTAAAAATACTCCTTAACCCCCAAAGTCACAAAAAAGAAGCTACCCAGGTTTATTA TGAGAAGGTTGAACCTCTGTTGAAGCTTGCAGGAATAAAAACTGATGTAACAAGATCTACCAATGTATTG GCACATTCTCTTCATGGAGTTCCTCATGTGATAACTGCAACATTGCACATTATAATGGGGCATGTACAGC TGGTCGACGTCTGCACCTTCAGCACCGCTGGCAAGCTTCTTCGCTTTGGGTTCTCAGCCATGTTTGGCTT TGGTGGAAGAACTTTGGCTCTGGCAGAAAAATATCGATGGATGTCCCCTAACCAACGGAGAGATTTTGCT GTTGTTAAGGCACTGGCAAAACTTAAGGCAGAAGACTGTGAAATATCATTTTTACCATTTAACAGCTCTG ATGATGTGCAAGAAAGGAGGGCACAGGGATCTCCCAAATCTGACTGTAATGATCAATGGCAAATGATCCA GGGTCAGTTCTTGAATGTCAGCATTATGGCAATTCCTTGCCTGTGTTCAGTGGCACCTAGAGGCTTGGCA CCTAATACCAGATTAAATAATGGAAGTATGGCTCTTATAATTGCCCGAAACACTTCTCGGCCAGAATTTA TAAAACACCTGAAAAGATATGCCAGTGTAAAAAATCAGTTCAATTTTCCATTTGTTGAGACTTACACTGT TGAGGAAGTAAAAGTTCATCCAAGGAATAATACTGGTGGATATAATCCAGAGGAGGAGGAGGATGAAACT GCTTCAGAAAATTGTTTCCCTTGGAATGTAGATGGTGACTTAATGGAAGTTGCATCAGAGGTCCATATTA GATTGCATCCAAGACTTATCAGTCTTTATGGAGGAAGCATGGAAGAAATGATTCCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218175 representing NM_001030313
Red=Cloning site Green=Tags(s) MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRW RPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDL INLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTRSTNVL AHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFA VVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLA PNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDET ASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001030313 |
| ORF Size | 1389 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001030313.2, NP_001025484.1 |
| RefSeq Size | 1392 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 375298 |
| UniProt ID | Q49MI3 |
| Protein Families | Druggable Genome |
| MW | 52.3 kDa |
| Gene Summary | This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218175L3 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218175L4 | Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| RG218175 | CERKL (tGFP-tagged) - Human ceramide kinase-like (CERKL), transcript variant 4 |
CNY 4,370.00 |
|
| SC302513 | CERKL (untagged)-Human ceramide kinase-like (CERKL), transcript variant 4 |
CNY 7,410.00 |
