ABCA12 (NM_015657) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARCI4A; ARCI4B; ICR2B; LI2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC218140 representing NM_015657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTACCTATATAAAAATTATTACTTCAGGTGACTCCGATAATATAACGCATGTGTGGAATGAGGATG ATGGACAGACCTTATCTCCAAGCAGTCTGGCTGCACAGCTCCTAATTCTGGAAAACTTTGAAGATGCCCT CTTAAATATATCAGCAAATAGTCCTTATATTCCTTACTTGGCATGTGTGAGAAATGTGACTGACAGTTTG GCCAGAGGTTCACCAGAAAATCTAAGACTCCTGCAGTCCACAATACGATTTAAAAAATCTTTTCTTCGCA ATGGTTCCTATGAAGATTACTTTCCTCCAGTTCCTGAAGTCCTAAAATCAAAACTGTCTCAACTTCGAAA CTTGACCGAACTTCTTTGTGAATCTGAAACTTTCAGTTTGATAGAGAAGTCATGCCAGCTCTCTGATATG AGCTTTGGGAGCCTGTGTGAAGAAAGTGAGTTTGATCTGCAACTCCTCGAAGCGGCAGAGCTGGGCACCG AAATAGCAGCCAGCTTACTGTACCATGACAATGTCATATCTAAAAAAGTGAGAGATTTGCTGACTGGAGA TCCAAGCAAAATTAATTTAAATATGGATCAGTTTCTAGAACAGGCACTGCAAATGAATTACTTGGAAAAT ATCACTCAGTTAATACCGATCATAGAAGCCATGCTGCATGTCAATAACAGTGCAGATGCTTCTGAAAAGC CAGGTCAGTTACTAGAAATGTTTAAAAATGTTGAAGAGCTGAAAGAAGATTTAAGGAGAACAACAGGAAT GTCCAACAGGACTATTGACAAGTTGCTGGCCATTCCCATCCCTGATAATAGAGCTGAGATTATTTCTCAG GTGTTCTGGCTGCATTCCTGTGATACTAATATCACCACTCCCAAACTAGAAGATGCAATGAAAGAATTCT GCAACCTGTCTCTTTCAGAGAGATCCCGGCAGTCTTACCTCATCGGACTCACCCTTCTGCACTACTTAAA CATTTACAACTTCACATACAAGGTGTTTTTCCCGAGGAAAGATCAAAAGCCAGTAGAAAAGATGATGGAG CTCTTCATAAGACTAAAAGAGATTCTCAATCAGATGGCTTCTGGCACACATCCGCTGCTAGACAAAATGA GATCCCTGAAGCAAATGCATCTGCCCAGAAGTGTTCCATTAACACAGGCAATGTACAGAAGCAACCGAAT GAACACACCACAAGGATCATTTAGCACCATCTCCCAAGCATTATGTTCTCAAGGAATTACCACTGAATAT TTAACTGCCATGCTGCCCTCTTCCCAGAGGCCAAAAGGCAACCACACCAAGGATTTTTTGACTTATAAAT TAACTAAAGAGCAAATTGCTTCAAAATATGGAATTCCCATAAATTCCACACCATTTTGCTTCTCCCTTTA TAAAGACATCATTAACATGCCCGCTGGACCTGTGATTTGGGCTTTCTTGAAACCTATGTTGTTGGGAAGA ATTTTGTATGCACCATATAACCCAGTCACAAAGGCAATAATGGAAAAGTCCAATGTAACTCTGAGACAGC TGGCGGAATTAAGAGAAAAATCTCAAGAGTGGATGGATAAGTCGCCACTTTTCATGAATTCCTTCCATCT GTTAAACCAGGCAATTCCAATGCTCCAGAATACTCTAAGGAACCCTTTTGTGCAAGTTTTTGTAAAGTTC TCCGTGGGACTCGATGCTGTTGAACTATTGAAACAGATAGATGAACTCGATATTCTAAGACTGAAATTAG AGAACAACATTGACATCATCGATCAGCTTAACACACTATCTTCCCTGACAGTAAATATTTCCTCTTGTGT ATTATATGACCGTATTCAGGCAGCAAAAACCATAGATGAAATGGAGAGAGAGGCTAAAAGGCTCTACAAA AGCAACGAACTCTTTGGAAGTGTTATTTTTAAGCTTCCTTCTAACAGAAGCTGGCACAGAGGCTATGACT CTGGAAATGTCTTTCTTCCTCCTGTCATAAAATATACCATCCGGATGAGTCTCAAGACCGCACAGACCAC AAGAAGCCTAAGAACCAAGATTTGGGCTCCAGGGCCACACAATTCTCCATCACACAACCAGATCTATGGC AGGGCTTTTATTTATTTACAGGATAGTATTGAAAGAGCAATCATTGAATTGCAAACTGGAAGGAACTCCC AGGAAATAGCAGTCCAGGTTCAAGCAATTCCTTATCCCTGCTTCATGAAAGACAACTTCCTAACCAGTGT CTCTTATTCTCTTCCAATTGTGCTTATGGTTGCCTGGGTTGTATTTATAGCTGCCTTTGTAAAAAAGCTT GTCTATGAGAAAGACCTCCGGCTTCATGAGTACATGAAGATGATGGGTGTGAACTCCTGCAGCCATTTCT TTGCCTGGCTTATAGAGAGTGTTGGATTTTTACTGGTTACCATCGTGATCCTCATCATTATACTCAAGTT TGGCAATATTCTTCCTAAAACAAATGGGTTCATTTTGTTCCTGTATTTTTCGGACTACAGCTTCTCGGTT ATTGCCATGAGCTATCTTATCAGTGTCTTCTTCAACAACACCAACATTGCAGCTCTGATCGGAAGCCTCA TCTACATCATTGCCTTCTTTCCATTTATTGTTCTGGTTACAGTGGAGAATGAGTTGAGCTATGTATTGAA AGTGTTCATGAGCCTGCTGTCCCCAACAGCATTCAGCTATGCAAGCCAATACATTGCACGATACGAAGAA CAGGGCATTGGTCTTCAGTGGGAAAATATGTACACCTCCCCGGTTCAGGATGACACCACCTCATTTGGCT GGCTGTGCTGTCTAATCCTAGCTGACTCTTTCATTTATTTCCTTATTGCTTGGTATGTCAGGAATGTCTT CCCAGGGACATACGGTATGGCAGCTCCCTGGTATTTTCCAATTCTTCCTTCCTATTGGAAGGAGCGATTT GGGTGTGCAGAGGTGAAGCCTGAGAAGAGCAATGGCCTCATGTTTACTAACATCATGATGCAGAACACCA ACCCATCTGCCAGTCCTGAATACATGTTTTCCTCTAACATCGAGCCTGAACCTAAAGATCTCACAGTCGG GGTTGCCCTGCATGGGGTCACAAAGATCTATGGCTCAAAAGTTGCTGTTGATAACCTCAATCTGAACTTT TATGAAGGGCATATTACTTCATTGCTGGGGCCCAATGGAGCTGGGAAAACTACTACCATTTCCATGTTAA CTGGGCTGTTTGGGGCCTCAGCAGGCACCATTTTTGTATATGGAAAAGATATCAAAACAGACCTACACAC GGTACGGAAGAACATGGGAGTCTGTATGCAGCACGACGTCTTGTTCAGTTACCTCACTACTAAGGAGCAC CTTCTCCTATATGGTTCCATCAAAGTTCCTCACTGGACTAAAAAGCAGCTCCACGAGGAAGTAAAAAGGA CTTTAAAAGATACTGGACTATATAGCCATCGTCATAAGAGAGTTGGAACACTGTCAGGAGGCATGAAGAG GAAGTTATCTATATCCATAGCTCTCATTGGTGGATCAAGGGTAGTAATTTTGGATGAACCATCTACTGGA GTTGACCCATGTTCTCGCCGAAGTATATGGGATGTTATATCCAAGAACAAAACTGCCAGAACAATCATTC TGTCAACGCACCACTTGGACGAGGCTGAAGTGCTGAGTGACCGCATCGCCTTCCTGGAGCAGGGTGGGCT TAGGTGCTGTGGGTCCCCATTTTACCTCAAGGAAGCCTTTGGCGATGGGTATCACCTCACGCTTACCAAG AAGAAGAGTCCAAATTTAAATGCAAATGCAGTATGTGACACCATGGCCGTGACAGCAATGATCCAATCAC ATCTCCCCGAAGCCTACCTCAAGGAGGATATTGGGGGAGAGCTTGTTTATGTACTTCCTCCATTCAGCAC CAAAGTCTCAGGGGCCTACCTGTCACTCCTACGGGCACTCGACAATGGCATGGGTGACCTCAACATCGGG TGCTACGGCATTTCAGATACCACCGTGGAGGAGGTCTTTCTGAACTTGACCAAAGAGTCACAAAAAAATA GTGCTATGAGTCTTGAGCACTTAACACAAAAGAAAATTGGGAATTCCAATGCCAATGGCATCTCAACTCC TGACGATTTATCTGTGAGCAGCAGCAATTTCACAGACAGAGATGACAAAATCCTGACAAGAGGAGAGAGG CTGGATGGCTTTGGACTGTTGCTGAAGAAGATCATGGCTATACTCATCAAGAGGTTCCACCACACCCGCA GGAACTGGAAAGGTCTCATTGCTCAGGTTATCCTCCCCATCGTCTTTGTTACCACTGCCATGGGCCTTGG CACACTGAGAAATTCCAGCAACAGTTATCCAGAGATTCAGATCTCCCCCTCTCTTTATGGTACCTCCGAA CAGACAGCCTTCTATGCTAATTATCACCCGAGCACGGAAGCACTTGTCTCAGCAATGTGGGACTTCCCTG GAATTGACAACATGTGTCTGAACACCAGTGATCTACAGTGTTTAAACAAAGACAGTCTGGAAAAATGGAA CACCAGTGGAGAACCCATCACTAATTTTGGTGTTTGCTCCTGCTCAGAAAATGTCCAGGAATGTCCTAAA TTTAACTATTCCCCACCGCACAGAAGAACTTACTCATCCCAGGTAATTTATAACCTCACTGGGCAACGAG TGGAAAATTATCTTATATCAACTGCAAATGAGTTTGTCCAAAAAAGATATGGAGGTTGGAGTTTTGGGCT GCCTTTGACAAAAGACCTTCGTTTTGATATAACAGGAGTCCCTGCCAATAGAACACTTGCCAAGGTATGG TATGATCCAGAAGGCTATCACTCCCTTCCAGCTTACCTCAACAGCCTGAATAATTTCCTTCTGCGAGTTA ACATGTCAAAATACGATGCTGCCCGACATGGCATCATCATGTATAGCCATCCTTATCCAGGAGTGCAAGA CCAAGAACAAGCCACAATCAGCAGTTTAATCGATATTTTAGTGGCACTGTCTATCTTGATGGGCTACTCT GTCACCACCGCCAGCTTTGTCACCTATGTTGTAAGGGAACATCAAACCAAAGCCAAACAGTTGCAGCACA TTTCAGGCATTGGCGTGACATGCTACTGGGTAACAAACTTCATTTATGACATGGTTTTCTACTTGGTGCC TGTAGCGTTTTCAATTGGTATCATTGCGATTTTCAAATTACCTGCATTCTACAGTGAAAACAACCTAGGC GCTGTATCTCTCCTACTTCTCCTGTTTGGGTATGCAACATTTTCCTGGATGTACTTGCTGGCTGGGCTCT TCCATGAAACAGGAATGGCCTTCATCACTTACGTCTGTGTCAACTTGTTTTTTGGCATTAATTCCATTGT TTCCCTGTCAGTGGTATACTTTCTTTCCAAGGAAAAGCCTAATGATCCGACTTTAGAACTTATTTCTGAA ACCCTCAAGCGCATTTTCCTGATTTTCCCACAATTCTGTTTTGGCTACGGTTTGATTGAACTTTCTCAAC AACAGTCGGTCCTAGACTTCTTAAAAGCATATGGAGTGGAATACCCAAATGAAACCTTTGAGATGAATAA ACTAGGTGCAATGTTTGTGGCTTTGGTTTCTCAGGGCACCATGTTTTTTTCCTTGCGACTCTTAATCAAC GAATCCCTGATAAAGAAACTCAGGCTTTTCTTCAGAAAATTTAATTCTTCACATGTAAGGGAGACAATAG ATGAGGATGAAGATGTGCGGGCTGAGAGATTAAGAGTTGAGAGTGGTGCAGCTGAATTTGACTTGGTCCA ACTTTATTGTCTCACAAAGACCTACCAACTTATCCACAAAAAGATTATAGCTGTAAACAACATCAGCATT GGGATACCTGCTGGAGAGTGTTTTGGGCTTCTTGGAGTGAATGGAGCAGGAAAGACCACTATATTCAAGA TGCTGACAGGAGACATCATTCCTTCAAGTGGAAACATTCTGATCAGAAATAAGACCGGATCTCTGGGTCA CGTTGATTCTCACAGCTCATTAGTTGGCTACTGTCCTCAGGAAGATGCCTTAGATGACCTGGTAACTGTG GAAGAACATTTGTATTTCTATGCCAGGGTACATGGAATTCCAGAAAAGGATATTAAAGAAACTGTTCATA AACTCCTTAGGAGACTTCACCTGATGCCCTTCAAGGACAGAGCTACCTCTATGTGCAGTTATGGCACAAA AAGAAAATTATCCACTGCACTGGCCTTGATAGGGAAACCTTCCATTCTACTGCTGGATGAGCCGAGCTCT GGCATGGATCCGAAGTCGAAACGGCACCTCTGGAAGATCATTTCAGAAGAAGTACAGAACAAATGTTCCG TCATCCTCACATCTCACAGCATGGAAGAATGTGAAGCTCTCTGTACCAGGTTGGCCATTATGGTGAATGG AAAGTTTCAATGTATTGGATCTTTGCAGCACATAAAGAGCAGGTTTGGACGAGGATTTACTGTCAAAGTT CACTTGAAGAATAACAAAGTGACCATGGAGACCCTCACAAAGTTCATGCAGCTGCACTTTCCAAAAACAT ACTTAAAAGATCAGCACCTCAGCATGCTAGAGTATCATGTACCAGTCACAGCAGGAGGAGTCGCAAACAT TTTTGATCTGCTGGAAACCAACAAGACTGCTTTAAATATTACAAATTTCTTAGTGAGTCAGACCACTCTG GAAGAGGTTTTCATCAACTTTGCCAAAGACCAGAAGTCCTATGAAACTGCTGATACCAGCAGCCAAGGTT CCACTATAAGTGTTGACTCACAAGATGACCAGATGGAGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC218140 representing NM_015657 Red=Cloning site Green=Tags(s) MFTYIKIITSGDSDNITHVWNEDDGQTLSPSSLAAQLLILENFEDALLNISANSPYIPYLACVRNVTDSL ARGSPENLRLLQSTIRFKKSFLRNGSYEDYFPPVPEVLKSKLSQLRNLTELLCESETFSLIEKSCQLSDM SFGSLCEESEFDLQLLEAAELGTEIAASLLYHDNVISKKVRDLLTGDPSKINLNMDQFLEQALQMNYLEN ITQLIPIIEAMLHVNNSADASEKPGQLLEMFKNVEELKEDLRRTTGMSNRTIDKLLAIPIPDNRAEIISQ VFWLHSCDTNITTPKLEDAMKEFCNLSLSERSRQSYLIGLTLLHYLNIYNFTYKVFFPRKDQKPVEKMME LFIRLKEILNQMASGTHPLLDKMRSLKQMHLPRSVPLTQAMYRSNRMNTPQGSFSTISQALCSQGITTEY LTAMLPSSQRPKGNHTKDFLTYKLTKEQIASKYGIPINSTPFCFSLYKDIINMPAGPVIWAFLKPMLLGR ILYAPYNPVTKAIMEKSNVTLRQLAELREKSQEWMDKSPLFMNSFHLLNQAIPMLQNTLRNPFVQVFVKF SVGLDAVELLKQIDELDILRLKLENNIDIIDQLNTLSSLTVNISSCVLYDRIQAAKTIDEMEREAKRLYK SNELFGSVIFKLPSNRSWHRGYDSGNVFLPPVIKYTIRMSLKTAQTTRSLRTKIWAPGPHNSPSHNQIYG RAFIYLQDSIERAIIELQTGRNSQEIAVQVQAIPYPCFMKDNFLTSVSYSLPIVLMVAWVVFIAAFVKKL VYEKDLRLHEYMKMMGVNSCSHFFAWLIESVGFLLVTIVILIIILKFGNILPKTNGFILFLYFSDYSFSV IAMSYLISVFFNNTNIAALIGSLIYIIAFFPFIVLVTVENELSYVLKVFMSLLSPTAFSYASQYIARYEE QGIGLQWENMYTSPVQDDTTSFGWLCCLILADSFIYFLIAWYVRNVFPGTYGMAAPWYFPILPSYWKERF GCAEVKPEKSNGLMFTNIMMQNTNPSASPEYMFSSNIEPEPKDLTVGVALHGVTKIYGSKVAVDNLNLNF YEGHITSLLGPNGAGKTTTISMLTGLFGASAGTIFVYGKDIKTDLHTVRKNMGVCMQHDVLFSYLTTKEH LLLYGSIKVPHWTKKQLHEEVKRTLKDTGLYSHRHKRVGTLSGGMKRKLSISIALIGGSRVVILDEPSTG VDPCSRRSIWDVISKNKTARTIILSTHHLDEAEVLSDRIAFLEQGGLRCCGSPFYLKEAFGDGYHLTLTK KKSPNLNANAVCDTMAVTAMIQSHLPEAYLKEDIGGELVYVLPPFSTKVSGAYLSLLRALDNGMGDLNIG CYGISDTTVEEVFLNLTKESQKNSAMSLEHLTQKKIGNSNANGISTPDDLSVSSSNFTDRDDKILTRGER LDGFGLLLKKIMAILIKRFHHTRRNWKGLIAQVILPIVFVTTAMGLGTLRNSSNSYPEIQISPSLYGTSE QTAFYANYHPSTEALVSAMWDFPGIDNMCLNTSDLQCLNKDSLEKWNTSGEPITNFGVCSCSENVQECPK FNYSPPHRRTYSSQVIYNLTGQRVENYLISTANEFVQKRYGGWSFGLPLTKDLRFDITGVPANRTLAKVW YDPEGYHSLPAYLNSLNNFLLRVNMSKYDAARHGIIMYSHPYPGVQDQEQATISSLIDILVALSILMGYS VTTASFVTYVVREHQTKAKQLQHISGIGVTCYWVTNFIYDMVFYLVPVAFSIGIIAIFKLPAFYSENNLG AVSLLLLLFGYATFSWMYLLAGLFHETGMAFITYVCVNLFFGINSIVSLSVVYFLSKEKPNDPTLELISE TLKRIFLIFPQFCFGYGLIELSQQQSVLDFLKAYGVEYPNETFEMNKLGAMFVALVSQGTMFFSLRLLIN ESLIKKLRLFFRKFNSSHVRETIDEDEDVRAERLRVESGAAEFDLVQLYCLTKTYQLIHKKIIAVNNISI GIPAGECFGLLGVNGAGKTTIFKMLTGDIIPSSGNILIRNKTGSLGHVDSHSSLVGYCPQEDALDDLVTV EEHLYFYARVHGIPEKDIKETVHKLLRRLHLMPFKDRATSMCSYGTKRKLSTALALIGKPSILLLDEPSS GMDPKSKRHLWKIISEEVQNKCSVILTSHSMEECEALCTRLAIMVNGKFQCIGSLQHIKSRFGRGFTVKV HLKNNKVTMETLTKFMQLHFPKTYLKDQHLSMLEYHVPVTAGGVANIFDLLETNKTALNITNFLVSQTTL EEVFINFAKDQKSYETADTSSQGSTISVDSQDDQMES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_015657
ORF Size	6831 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015657.3, NP_056472.2
RefSeq Size	8097 bp
RefSeq ORF	6834 bp
Locus ID	26154
UniProt ID	Q86UK0
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ABC transporters
MW	256.8 kDa
Gene Summary	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily, which is the only major ABC subfamily found exclusively in multicellular eukaryotes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

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