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ATP6V1H (NM_213619) Human Tagged ORF Clone
CAT＃: RC218081
- TrueORF®
ATP6V1H (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2
ORF Plasmid: tGFP
"NM_213619" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-11; MSTP042; NBP1; SFD; SFDalpha; SFDbeta; VMA13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218081 representing NM_213619
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAAATGGATATCCGAGGTGCTGTGGATGCTGCTGTCCCCACCAATATTATTGCTGCCAAGGCTG CAGAAGTTCGTGCAAACAAAGTCAACTGGCAATCCTATCTTCAGGGACAGATGATTTCTGCTGAAGATTG TGAGTTTATTCAGAGGTTTGAAATGAAACGAAGCCCTGAAGAGAAGCAAGAGATGCTTCAAACTGAAGGC AGCCAGTGTGCTAAAACATTTATAAATCTGATGACTCATATCTGCAAAGAACAGACCGTTCAGTATATAC TAACTATGGTGGATGATATGCTGCAGGAAAATCATCAGCGTGTTAGCATTTTCTTTGACTATGCAAGATG TAGCAAGAACACTGCGTGGCCCTACTTTCTGCCAATGTTGAATCGCCAGGATCCCTTCACTGTTCATATG GCAGCAAGAATTATTGCCAAGTTAGCAGCTTGGGGAAAAGAACTGATGGAAGGCAGTGACTTAAATTACT ATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAGTTCGCAGTATGTGCAGTGCGTGGCCGGGTGTTT GCAGCTGATGCTCCGGGTCAATGAGTACCGCTTTGCTTGGGTGGAAGCAGATGGGGTAAATTGCATAATG GGAGTGTTGAGTAACAAGTGTGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCCTGGCAT TCAGTCCTCAAATGTGTGAACACCTGCGGCGCTATAATATCATTCCAGTTCTGTCTGATATCCTTCAGGA GTCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGCATTTCGTAACTTTTTAGAAAAATCAACTGAA AGAGAAACTCGCCAAGAATATGCCCTGGCTATGATTCAGTGCAAAGTTCTGAAACAGTTGGAGAACTTGG AACAGCAGAAGTACGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAAAACTTGGAGAGAG TGTCCAGGACCTTAGTTCATTTGATGAATACAGTTCAGAACTTAAATCTGGAAGGTTGGAATGGAGTCCT GTGCACAAATCTGAGAAATTTTGGAGAGAGAATGCTGTGAGGTTAAATGAGAAGAATTATGAACTCTTGA AAATCTTGACAAAACTTTTGGAAGTGTCAGATGATCCCCAAGTCTTAGCTGTTGCTGCTCACGATGTTGG AGAATATGTGCGGCATTATCCACGAGGCAAACGGGTCATCGAGCAGCTCGGTGGGAAGCAGCTGGTCATG AACCACATGCATCATGAAGACCAGCAGGTCCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATGGTGC ACAACTGGGAATACCTTGGCAAGCAGCTCCAGTCCGAGCAGCCCCAGACCGCTGCCGCCCGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218081 representing NM_213619
Red=Cloning site Green=Tags(s) MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPEEKQEMLQTEG SQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHM AARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIM GVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTE RETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSP VHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVM NHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_213619 |
| ORF Size | 1395 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213619.2, NP_998784.1 |
| RefSeq Size | 2111 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 51606 |
| UniProt ID | Q9UI12 |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 54 kDa |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218081L1 | Lenti-ORF clone of ATP6V1H (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 7,888.00 |
|
| RC218081L2 | Lenti-ORF clone of ATP6V1H (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 5,890.00 |
|
| RC218081L3 | Lenti-ORF clone of ATP6V1H (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 5,890.00 |
|
| RC218081L4 | Lenti-ORF clone of ATP6V1H (mGFP-tagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 5,890.00 |
|
| RG218081 | ATP6V1H (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 7,088.00 |
|
| SC308682 | ATP6V1H (untagged)-Human ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H), transcript variant 2 |
CNY 7,410.00 |
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