GPR86 (P2RY13) (NM_023914) Human Tagged ORF Clone

CAT#: RC217904

P2RY13 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 13 (P2RY13), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_023914" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-P2Y13 Receptor
    • 50 ul

CNY 11,000.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FKSG77; G-protein coupled receptor 94; GPCR1; GPCR1, GPR86, GPR94, P2Y13, SP174, FKSG77; GPR86; GPR94; G protein-coupled receptor 86; P2Y13; P2Y purinoceptor 13; purinergic receptor P2Y, G-protein coupled, 13; SP174
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217904 representing NM_023914
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACTCGGATAGTACAGC
TGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTGCTGAATACTTTGGCTCTGTGGGT
GTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATCTACCTCAAAAACACTTTGGTGGCCGACTTGATA
ATGACACTCATGCTTCCTTTCAAAATCCTCTCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTG
TGTGTCGTTTTTCTTCGGTGATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGC
CTTTGACAGATTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCAAAA
ACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATATGATCTTGAGCAACAAGG
AAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCTCTGGGGCTGAAATGGCATCAAAT
GGTAAATAACATATGCCAGTTTATTTTCTGGACTGTTTTTATCCTAATGCTTGTGTTTTATGTGGTTATT
GCAAAAAAAGTATATGATTCTTATAGAAAGTCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAG
GCAAAGTATTTGTTGTCGTGGCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATA
TACTCACAGTCAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAAACA
ACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTATGTAAAAAATTCA
CAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGCCAAGAAAATCATAGCAGTCAGAC
AGACAACATAACCTTAGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217904 representing NM_023914
Red=Cloning site Green=Tags(s)

MNTTVMQGFNRSERCPRDTRIVQLVFPALYTVVFLTGILLNTLALWVFVHIPSSSTFIIYLKNTLVADLI
MTLMLPFKILSDSHLAPWQLRAFVCRFSSVIFYETMYVGIVLLGLIAFDRFLKIIRPLRNIFLKKPVFAK
TVSIFIWFFLFFISLPNMILSNKEATPSSVKKCASLKGPLGLKWHQMVNNICQFIFWTVFILMLVFYVVI
AKKVYDSYRKSKSKDRKNNKKLEGKVFVVVAVFFVCFAPFHFARVPYTHSQTNNKTDCRLQNQLFIAKET
TLFLAATNICMDPLIYIFLCKKFTEKLPCMQGRKTTASSQENHSSQTDNITLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023914
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023914.1
RefSeq Size 2858 bp
RefSeq ORF 1001 bp
Locus ID 53829
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 38.9 kDa
Gene Summary The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is activated by ADP. [provided by RefSeq, Sep 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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