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l Afadin (AFDN) (NM_001040000) Human Tagged ORF Clone
CAT＃: RC217774
MLLT4 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (MLLT4), transcript variant 2
ORF Plasmid: tGFP
"NM_001040000" in other vectors (4)
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CNY 16,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AF6; l-afadin; MLL-AF6; MLLT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217774 representing NM_001040000
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGCGGCCGTGACGAGGAGCGGCGGAAGCTGGCCGACATCATCCACCACTGGAACGCCAACC GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTCCATGGAGTGATGAGATTTTATTT TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTGCCACCACTCAAGAT GTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCTCCCAAGTATTCACTCT ATGAAGTGCATGTCAGCGGAGAAAGAAGATTGGATATAGATGAGAAACCTCTAGTTGTACAACTGAATTG GAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCATTCCTCCTAAGAAGGCT CAAAGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAAGAGAACTCTCTCAAAGA AAGAAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCTGATAAAGATGATAGACC TTTCCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTTTACAAAGACATGCCGGAAACCAGC TTTACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGGAGGCAGCAAAAATTGGAAAAGAGAA TGCAGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGAACATTGAGAATTTATGCAGATAGTTT AAAACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTGGCTGAA GCTTTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGCCCGGGTTATGCTTCCTC CTGGAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGATGATGAGTGTCCTTTACA AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGGCCACCAGACCAC ATCCCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACACCCAAGGGAAAGGAGAGAGCTGACGGGTCTG GCTATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTATTTAGTAGAGTTAAGCCCAGGGAGAAGGAATCA CTTTGCCTACTACAACTATCACACTTACGAAGATGGTTCTGACTCTAGAGATAAGCCAAAGCTTTACCGC CTTCAGTTAAGTGTTACTGAAGTTGGGACAGAAAAGTTGGATGACAACTCTATCCAGTTGTTTGGCCCAG GAATTCAGCCCCATCACTGTGACCTTACCAACATGGATGGAGTGGTCACTGTGACGCCCAGAAGTATGGA CGCAGAAACCTACGTGGAAGGCCAGCGCATCTCAGAAACCACCATGCTGCAGAGTGGCATGAAAGTGCAG TTTGGGGCGTCCCATGTATTTAAGTTTGTGGACCCCAGTCAGGATCATGCTCTTGCAAAAAGATCTGTGG ATGGAGGCCTGATGGTTAAGGGCCCAAGACATAAACCTGGAATTGTTCAGGAGACAACTTTTGATTTGGG AGGAGATATTCATAGTGGGACAGCATTACCGACAAGCAAGAGCACCACTAGGCTGGACAGCGACAGAGTG TCGTCTGCCTCTAGCACAGCCGAGCGGGGAATGGTGAAGCCGATGATCAGAGTAGAACAGCAGCCAGATT ATCGCAGGCAAGAAAGCAGAACACAGGATGCTTCTGGGCCTGAGCTGATACTACCTGCAAGCATTGAATT CAGGGAAAGTTCTGAAGATTCATTTTTGTCTGCCATTATAAATTATACTAATAGCTCTACAGTCCACTTT AAGTTGTCCCCTACATATGTATTATATATGGCATGCCGGTATGTATTGTCCAACCAGTACAGACCTGACA TCAGCCCTACAGAGCGCACACATAAAGTCATTGCAGTCGTCAACAAGATGGTGAGCATGATGGAGGGTGT CATCCAGAAACAGAAGAATATTGCAGGGGCACTTGCCTTCTGGATGGCAAATGCATCTGAACTTCTCAAC TTCATTAAGCAAGACCGAGACCTTAGTCGGATCACACTGGATGCTCAAGATGTTTTAGCACATTTGGTTC AAATGGCATTTAAATACTTGGTTCACTGTCTTCAATCAGAACTTAATAATTACATGCCAGCCTTTCTAGA TGACCCTGAAGAGAACAGTCTGCAACGACCAAAAATAGATGATGTGCTGCACACGCTCACAGGAGCCATG TCCTTGCTACGACGCTGCAGAGTCAATGCCGCCCTGACCATCCAGCTCTTCTCTCAGCTCTTCCACTTCA TCAATATGTGGCTGTTCAATAGATTGGTGACCGACCCAGATTCGGGGCTGTGCTCCCATTACTGGGGTGC GATTATCCGTCAGCAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCAGGGGCTGGAACTGGCTGCGGAC TGTCATCTGAGCAGGATCGTGCAGGCAACGACTTTGCTTACCATGGATAAGTATGCACCTGATGACATTC CAAATATAAACAGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAGCCTTATTACAGAACTATCACTG TGCACCTGATGAGCCTTTTATCCCAACGGATCTTATAGAAAATGTAGTGACTGTGGCTGAAAACACTGCC GATGAGCTGGCCCGCAGTGATGGAAGGGAAGTGCAGTTGGAGGAGGATCCTGATCTGCAGCTGCCGTTTC TTTTGCCAGAAGATGGTTATTCTTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGA CCCTCTGTGCCAGAGAGGATTTTGCAGGTTAATTCCTCACACACGTTCACCAGGTACTTGGACAATATAT TTTGAAGGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAGCTGGCTCAGCCTCTGAGGAAAG AACCTGAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCCTTAGCATTGTTGCAGCAAAGGG TGCTGGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGGAGGTGCTGCAGATGTGGATGGA CGTCTAGCTGCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTCTCTCAGGAAAGGG CGGCAGAACTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAAAGCAGGGTGCCATCTACCA CGGTCTGGCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTCAGATCGTCGTGGCTCAGGT AAACCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAAAATGGGTCTCCTGAGAGTC CTCAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATGACAGACTGATGAAAAATAG AGCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGGAGGGAAAAGTGCATATGCC TCTGGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACTGAGGAGCAGACGCCTCCGC CTAGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATTTTACCTTCCCAGCTTCCAA ATCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGAAAAGCCACATATGCACACA GATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAAGAACTTCGAGAAGATAAAG CTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTGATAGTGATATGTGGATAAA TCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCATTCCTCTAAGTCGGTCACC CCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCAGCCATACCGGCCACCCCTG TGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCACCTCCAGTCCACTATGCCGG TGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGCCAACCAGATAGGGCTGCCG TCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGTTGGTATGAGAAGGAGAAGG CCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGTTGGGCCAGATGCGCACTCA GTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCCCGAAAAGCCTTCCACACTC CAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCCCGCACGATCGAGCGCAGAG ACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTCTGTCCCCCGACCCGTGGAA GCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGACATGCTGAGCAAGGAGATC CAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTGCGCAAGCTCATGCTGGAGT GGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACGAGGAGGAGGAGGACGATGA TGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAGGGACGAGGAGCGGAGGCGG CAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGAGCGAGGCAAGAGGAAGAGCGCCGGC GGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGGTTATGGTCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217774 representing NM_001040000
Red=Cloning site Green=Tags(s) MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQD VIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKA QSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETS FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAE ALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDH IPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYR LQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQ FGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRV SSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHF KLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLN FIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAM SLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAAD CHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTA DELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIY FEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDG RLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSG KPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYA SGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHT DSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVT PASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLP SAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTL QRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEI QELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRR QQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040000 |
| ORF Size | 4953 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040000.3 |
| RefSeq Size | 7629 bp |
| RefSeq ORF | 4956 bp |
| Locus ID | 4301 |
| UniProt ID | P55196 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Leukocyte transendothelial migration, Tight junction |
| MW | 187.7 kDa |
| Gene Summary | This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217774L3 | Lenti-ORF clone of MLLT4 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 (MLLT4), transcript variant 2 |
CNY 13,970.00 |
|
| RC217774L4 | Lenti-ORF clone of MLLT4 (mGFP-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 (MLLT4), transcript variant 2 |
CNY 13,970.00 |
|
| RG217774 | MLLT4 (tGFP-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 (MLLT4), transcript variant 2 |
CNY 18,544.00 |
|
| SC311059 | MLLT4 (untagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (MLLT4), transcript variant 2 |
CNY 16,952.00 |
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