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ROCK2 (NM_004850) Human Tagged ORF Clone
CAT＃: RC217764
ROCK2 (Myc-DDK-tagged)-Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2)
ORF Plasmid: tGFP
"NM_004850" in other vectors (7)
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CNY 14,248.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ROCK-II |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217764 representing NM_004850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCAGGCG CGAGCCGCCAGAGGAAGCTGGAGGCGCTGATCCGAGACCCTCGCTCCCCCATCAACGTGGAGAGCTTGCT GGATGGCTTAAATTCCTTGGTCCTTGATTTAGATTTTCCTGCTTTGAGGAAAAACAAGAACATAGATAAT TTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTCTACAGATGAAGGCAGAAGACTATGATG TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTGCAGTTGGTTCGTCACAAGGCATCGCAGAAGGT TTATGCTATGAAGCTTCTTAGTAAGTTTGAAATGATAAAAAGATCAGATTCTGCCTTTTTTTGGGAAGAA AGAGATATTATGGCCTTTGCCAATAGCCCCTGGGTGGTTCAGCTTTTTTATGCCTTTCAAGATGATAGGT ATCTGTACATGGTAATGGAGTACATGCCTGGTGGAGACCTTGTAAACCTTATGAGTAATTATGATGTGCC TGAAAAATGGGCCAAATTTTACACTGCTGAAGTTGTTCTTGCTCTGGATGCAATACACTCCATGGGTTTA ATACACAGAGATGTGAAGCCTGACAACATGCTCTTGGATAAACATGGACATCTAAAATTAGCAGATTTTG GCACGTGTATGAAGATGGATGAAACAGGCATGGTACATTGTGATACAGCAGTTGGAACACCGGATTATAT ATCACCTGAGGTTCTGAAATCACAAGGGGGTGATGGTTTCTATGGGCGAGAATGTGATTGGTGGTCTGTA GGTGTTTTCCTTTATGAGATGCTAGTGGGGGATACTCCATTTTATGCGGATTCACTTGTAGGAACATATA GCAAAATTATGGATCATAAGAATTCACTGTGTTTCCCTGAAGATGCAGAAATTTCCAAACATGCAAAGAA TCTCATCTGTGCTTTCTTAACAGATAGGGAGGTACGACTTGGGAGAAATGGGGTGGAAGAAATCAGACAG CATCCTTTCTTTAAGAATGATCAGTGGCATTGGGATAACATAAGAGAAACGGCAGCTCCTGTAGTACCTG AACTCAGCAGTGACATAGACAGCAGCAATTTCGATGACATTGAAGATGACAAAGGAGATGTAGAAACCTT CCCAATTCCTAAAGCTTTTGTTGGAAATCAGCTGCCTTTCATCGGATTTACCTACTATAGAGAAAATTTA TTATTAAGTGACTCTCCATCTTGTAGAGAAACTGATTCCATACAATCAAGGAAAAATGAAGAAAGTCAAG AGATTCAGAAAAAACTGTATACATTAGAAGAACATCTTAGCAATGAGATGCAAGCCAAAGAGGAACTGGA ACAGAAGTGCAAATCTGTTAATACTCGCCTAGAAAAAACAGCAAAGGAGCTAGAAGAGGAGATTACCTTA CGGAAAAGTGTGGAATCAGCATTAAGACAGTTAGAAAGAGAAAAGGCGCTTCTTCAGCACAAAAATGCAG AATATCAGAGGAAAGCTGATCATGAAGCAGACAAAAAACGAAATTTGGAAAATGATGTTAACAGCTTAAA AGATCAACTTGAAGATTTGAAAAAAAGAAATCAAAACTCTCAAATATCCACTGAGAAAGTGAATCAACTC CAGAGACAACTGGATGAAACCAATGCTTTACTGCGAACAGAGTCTGATACTGCAGCCCGGTTAAGGAAAA CCCAGGCAGAAAGTTCAAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG CCTGCTGGAGACTGCCAAGTTAAAACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTCTAGAATCTGAA AGGAGGGATCGAACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATGTGGCCTAGAAGAAG ATTTAAAGAACGGCAAAATCTTACTAGCGAAAGTAGAACTGGAGAAGAGACAACTTCAGGAGAGATTTAC TGATTTGGAAAAGGAAAAAAGCAACATGGAAATAGATATGACATACCAACTAAAAGTTATACAGCAGAGC CTAGAACAAGAAGAAGCTGAACATAAGGCCACAAAGGCACGACTAGCAGATAAAAATAAGATCTATGAGT CCATCGAAGAAGCCAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTCTTGGAGGAAAGAACTTTAAA ACAGAAAGTGGAGAACCTATTGCTAGAAGCTGAGAAAAGATGTTCTCTATTAGACTGTGACCTCAAACAG TCACAGCAGAAAATAAATGAGCTCCTTAAACAGAAAGATGTGCTAAATGAGGATGTTAGAAACCTGACAT TAAAAATAGAGCAAGAAACTCAGAAGCGCTGCCTTACACAAAATGACCTGAAGATGCAAACACAACAGGT TAACACACTAAAAATGTCAGAAAAGCAGTTAAAGCAAGAAAATAACCATCTCATGGAAATGAAAATGAAC TTGGAAAAACAAAATGCTGAACTTCGAAAAGAACGTCAGGATGCAGATGGGCAAATGAAAGAGCTCCAGG ATCAGCTCGAAGCAGAACAGTATTTCTCAACCCTTTATAAAACACAAGTTAGGGAGCTTAAAGAAGAATG TGAAGAAAAGACCAAACTTGGTAAAGAATTGCAGCAGAAGAAACAGGAATTACAGGATGAACGGGACTCT TTGGCTGCCCAACTGGAGATCACCTTGACCAAAGCAGATTCTGAGCAACTGGCTCGTTCAATTGCTGAAG AACAATATTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAGATCAAAGAGATGATGGCTAGACA CAAACAGGAACTTACGGAAAAAGATGCTACAATTGCTTCTCTTGAGGAAACTAATAGGACACTAACTAGT GATGTTGCCAATCTTGCAAATGAGAAAGAAGAATTAAATAACAAATTGAAAGATGTTCAAGAGCAACTGT CAAGATTGAAAGATGAAGAAATAAGCGCAGCAGCTATTAAAGCACAGTTTGAGAAGCAGCTATTAACAGA AAGAACACTCAAAACTCAAGCTGTGAATAAGTTGGCTGAGATCATGAATCGAAAAGAACCTGTCAAGCGT GGTAATGACACAGATGTGCGGAGAAAAGAGAAGGAGAATAGAAAGCTACATATGGAGCTTAAATCTGAAC GTGAGAAATTGACCCAGCAGATGATCAAGTATCAGAAAGAACTGAATGAAATGCAGGCACAAATAGCTGA AGAGAGCCAGATTCGAATTGAACTGCAGATGACATTGGACAGTAAAGACAGTGACATTGAGCAGCTGCGG TCACAACTCCAAGCCTTGCATATTGGTCTGGATAGTTCCAGTATAGGCAGTGGACCAGGGGATGCTGAGG CAGATGATGGGTTTCCAGAATCAAGATTAGAAGGATGGCTTTCATTGCCTGTACGAAACAACACTAAGAA ATTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGTAAGAAGATTCTTTTCTATGACAGTGAACAAGAT AAAGAACAATCCAATCCTTACATGGTTTTAGATATAGACAAGTTATTTCATGTCCGACCAGTTACACAGA CAGATGTGTATAGAGCAGATGCTAAAGAAATTCCAAGGATATTCCAGATTCTGTATGCCAATGAAGGAGA AAGTAAGAAGGAACAAGAATTTCCAGTGGAGCCAGTTGGAGAAAAATCTAATTATATTTGCCACAAGGGA CATGAGTTTATTCCTACTCTTTATCATTTCCCAACCAACTGTGAGGCTTGTATGAAGCCCCTGTGGCACA TGTTTAAGCCTCCTCCTGCTTTGGAGTGCCGCCGTTGCCATATTAAGTGTCATAAAGATCATATGGACAA AAAGGAGGAGATTATAGCACCTTGCAAAGTATATTATGATATTTCAACGGCAAAGAATCTGTTATTACTA GCAAATTCTACAGAAGAGCAGCAGAAGTGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCCCCAG CTCCAGACCCTTTTGCCCGATCATCTCCTAGAACTTCAATGAAGATACAGCAAAACCAGTCTATTAGACG GCCAAGTCGACAGCTTGCCCCAAACAAACCTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217764 representing NM_004850
Red=Cloning site Green=Tags(s) MSRPPPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDN FLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEE RDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGL IHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSV GVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQ HPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENL LLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITL RKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQL QRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESE RRDRTHGSEIINDLQGRICGLEEDLKNGKILLAKVELEKRQLQERFTDLEKEKSNMEIDMTYQLKVIQQS LEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQ SQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMN LEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLGKELQQKKQELQDERDS LAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTS DVANLANEKEELNNKLKDVQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKR GNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLR SQLQALHIGLDSSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQD KEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKG HEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLL ANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004850 |
| ORF Size | 4164 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004850.5 |
| RefSeq Size | 6401 bp |
| RefSeq ORF | 4167 bp |
| Locus ID | 9475 |
| UniProt ID | O75116 |
| Domains | pkinase, HR1, PH, M |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway |
| MW | 160.7 kDa |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Two functional polymorphisms of ROCK2 enhance arterial stiffening through inhibiting its activity and expression
,Liao, Y;Liu, P;Lin, H;Lin, W;Liao, JK;Juo, SH;,
J. Mol. Cell. Cardiol.
,PubMed ID 25481646
[ROCK2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217764L1 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), Myc-DDK-tagged |
CNY 16,648.00 |
|
| RC217764L2 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mGFP tagged |
CNY 15,770.00 |
|
| RC217764L3 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), Myc-DDK-tagged |
CNY 16,648.00 |
|
| RC217764L4 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mGFP tagged |
CNY 16,648.00 |
|
| RG217764 | ROCK2 (tGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) |
CNY 15,848.00 |
|
| SC117098 | ROCK2 (untagged)-Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) |
CNY 9,504.00 |
|
| SC323671 | ROCK2 (untagged)-Kinase deficient mutant (K121M) of Human Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) |
CNY 9,504.00 |
