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NDST3 (NM_004784) Human Tagged ORF Clone
CAT＃: RC217551
NDST3 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3)
ORF Plasmid: tGFP
"NM_004784" in other vectors (6)
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CNY 6,392.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSST3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217551 representing NM_004784
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTTATCATGAAGCTTCACAGACACTTTCAAAGAACAGTCATTCTGCTTGCCACTTTTTGTATGG TGAGCATTATCATTTCTGCTTACTACCTGTACAGTGGCTACAAACAGGAAAATGAACTCTCTGAGACGGC TTCAGAAGTTGACTGTGGCGACCTCCAACACCTACCATATCAACTAATGGAAGTGAAAGCAATGAAGCTT TTTGATGCCTCAAGGACAGACCCCACAGTCCTAGTATTTGTAGAGAGCCAGTACTCATCTCTTGGTCAAG ACATCATTATGATTCTAGAATCAAGTAGATTCCAGTATCACATTGAAATTGCCCCTGGAAAGGGAGATCT CCCAGTGCTTATAGACAAAATGAAAGGCAAATACATTCTCATTATTTATGAGAATATTTTAAAGTATATA AATATGGATTCCTGGAATCGAAGCCTTCTAGATAAATACTGTGTAGAATATGGTGTGGGTGTCATTGGAT TCCACAAAACTAGTGAGAAGAGTGTACAGAGCTTTCAGTTAAAAGGTTTCCCTTTTTCCATATATGGAAA TCTTGCAGTAAAAGATTGTTGTATTAATCCTCATTCTCCATTGATTCGTGTGACCAAATCTTCCAAGCTT GAAAAAGGTTCTTTACCTGGAACTGACTGGACAGTTTTTCAGATTAATCATTCAGCCTATCAACCAGTAA TATTTGCCAAAGTAAAGACCCCAGAAAACCTTTCTCCTTCCATCTCTAAAGGTGCTTTTTATGCCACTAT TATACATGACCTGGGGCTTCATGATGGAATTCAAAGGGTTCTTTTTGGCAACAACTTGAACTTTTGGCTG CACAAGCTCATCTTCATAGATGCCATCTCCTTCTTATCAGGGAAGAGGCTGACATTGTCCTTGGACAGGT ACATTCTTGTGGATATTGATGATATATTTGTGGGAAAAGAGGGAACAAGAATGAACACCAATGATGTAAA GGCCCTGCTTGATACTCAGAATCTTTTGCGTGCACAAATCACAAATTTTACATTCAACCTGGGATTTTCA GGGAAATTTTACCATACAGGAACTGAAGAGGAAGATGAAGGAGATGACTGTCTGTTGGGGTCTGTGGATG AGTTCTGGTGTTTTCCTCACATGTGGAGCCATATGCAGCCCCACCTCTTCCACAATGAGTCATCTTTGGT GGAGCAGATGATTCTCAACAAAAAATTTGCCTTAGAGCACGGCATTCCAACGGACATGGGCTACGCTGTG GCCCCTCACCATTCGGGCGTCTACCCTGTACATGTTCAGCTTTACGAGGCCTGGAAGAAGGTCTGGAATA TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAGCCAGCTAGATACCGGAGGGGTTTTATCCACAA AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTTTTCACTCACACCATTTTCTACAAAGAATATCCA GGGGGTCCTAAAGAGCTGGATAAGAGTATCCAAGGAGGAGAACTTTTCTTCACTGTCGTCCTCAACCCTA TCAGCATTTTCATGACCCATTTGTCCAACTATGGGAATGACCGACTGGGATTATATACATTTGTTAATCT GGCCAACTTTGTGAAGAGCTGGACCAACCTGCGACTTCAGACTCTGCCTCCAGTACAACTGGCCCACAAG TATTTTGAGCTGTTTCCTGATCAGAAAGACCCTCTCTGGCAGAATCCTTGCGATGACAAACGCCACAGAG ACATTTGGTCTAAAGAAAAAACTTGTGATCGCTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG TACCACTGCTTTGTATTTGTTCCTGGTTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAAACC TTTGAGGAGGTACAGTTCTTTAATAGAAATAACTACCACAGGGGGATTGATTGGTATATGGATTTCTTCC CAGTCCCATCTAATGTCACTACCGACTTTTTGTTTGAGAAGAGTGCCAATTACTTCCACTCAGAGGAAGC CCCTAAAAGAGCTGCTTCTCTGGTTCCCAAAGCCAAGATTATCACCATTCTCATTGACCCTTCAGACCGA GCATACTCCTGGTACCAGCATCAGCGATCACATGAAGATCCTGCAGCTCTGAAGTTTAGCTTCTACGAAG TGATCTCAGCAGGGCCCCGTGCACCCTCGGAGCTCAGAGCCTTGCAGAAGAGATGTTTGGTCCCGGGGTG GTATGCCAGCCACATCGAGAGATGGCTTGTTTATTTCCCCCCATTTCAGTTGCTAATTATTGATGGGCAA CAACTAAGAACTGATCCTGCTACAGTGATGGATGAAGTACAGAAGTTTCTAGGAGTCTTGCCTCATTATA ATTACTCAGAAGCTTTAACGTTTGATTCTCATAAAGGTTTCTGGTGTCAGTTACTGGAAGAAGGTAAAAC AAAATGCCTTGGAAAGAGCAAAGGAAGAAAATACCCTCCAATGGATTCTGATAGCAGGACATTTCTGTCA AGCTACTATCGAGATCACAACGTGGAACTCTCAAAGCTGCTGCACAAACTGGGTCAGCCTCTGCCATCCT GGCTGAGACAGGAGCTGCAGAAAGTAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217551 representing NM_004784
Red=Cloning site Green=Tags(s) MSFIMKLHRHFQRTVILLATFCMVSIIISAYYLYSGYKQENELSETASEVDCGDLQHLPYQLMEVKAMKL FDASRTDPTVLVFVESQYSSLGQDIIMILESSRFQYHIEIAPGKGDLPVLIDKMKGKYILIIYENILKYI NMDSWNRSLLDKYCVEYGVGVIGFHKTSEKSVQSFQLKGFPFSIYGNLAVKDCCINPHSPLIRVTKSSKL EKGSLPGTDWTVFQINHSAYQPVIFAKVKTPENLSPSISKGAFYATIIHDLGLHDGIQRVLFGNNLNFWL HKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRAQITNFTFNLGFS GKFYHTGTEEEDEGDDCLLGSVDEFWCFPHMWSHMQPHLFHNESSLVEQMILNKKFALEHGIPTDMGYAV APHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYP GGPKELDKSIQGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANFVKSWTNLRLQTLPPVQLAHK YFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALYLFLVMHPSILSNSPSPKT FEEVQFFNRNNYHRGIDWYMDFFPVPSNVTTDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDR AYSWYQHQRSHEDPAALKFSFYEVISAGPRAPSELRALQKRCLVPGWYASHIERWLVYFPPFQLLIIDGQ QLRTDPATVMDEVQKFLGVLPHYNYSEALTFDSHKGFWCQLLEEGKTKCLGKSKGRKYPPMDSDSRTFLS SYYRDHNVELSKLLHKLGQPLPSWLRQELQKVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004784 |
| ORF Size | 2619 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004784.3 |
| RefSeq Size | 3188 bp |
| RefSeq ORF | 2622 bp |
| Locus ID | 9348 |
| UniProt ID | O95803 |
| Domains | Sulfotransfer |
| Protein Families | Transmembrane |
| Protein Pathways | Heparan sulfate biosynthesis, Metabolic pathways |
| MW | 100.7 kDa |
| Gene Summary | This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin. [provided by RefSeq, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217551L1 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), Myc-DDK-tagged |
CNY 8,792.00 |
|
| RC217551L2 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), mGFP tagged |
CNY 9,030.00 |
|
| RC217551L3 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC217551L4 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), mGFP tagged |
CNY 9,030.00 |
|
| RG217551 | NDST3 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3) |
CNY 7,890.00 |
|
| SC310066 | NDST3 (untagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3) |
CNY 6,400.00 |
