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PIGW (NM_178517) Human Tagged ORF Clone
CAT＃: RC217028
- TrueORF®
PIGW (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_178517" in other vectors (4)
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CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gwt1; HPMRS5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217028 ORF sequence, codon optimized.
Due to the complexity of NM_178517, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAAAAGCAGATGAAGGAAGCTTTTGTCAGTAACCTCAATGGAACCACCGTGCTGGAAATCACCC AGGGATTGTGCTTTCCTGCATTCTGTATCCTGTGCAGAGGGTTCCTGATCATTTTCTCACAGTACTTGTG TTCTTTTTCACCTACCTGGAAAACTAGATTCCTCACTGACTTTGTTGTCCTAATAGTTCCCATGGTAGCC ACTTTGACCATTTGGGCTTCATTTATCCTCCTTGAGCTTCTCGGTGTAATTATCTTTGGGGCAGGGCTGT TGTATCAAATATACCGAAGGAGGACCTGCTATGCCAGACTGCCTTTCCTAAAAATCCTTGAAAAATTCTT GAACATCAGTCTAGAATCAGAATACAATCCAGCCATCTCCTGTTTCCGTGTAATTACCAGTGCGTTTACT GCTATTGCTATTTTGGCTGTGGACTTCCCACTTTTTCCCAGAAGATTTGCCAAAACTGAGCTCTATGGGA CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTGTTTTTGGGTCTGCAATGGTTTGTCTAGAGGTCAGGAG GAGAAAATATATGGAAGGGTCCAAATTGCATTACTTTACAAACTCATTGTACTCTGTTTGGCCATTAGTC TTCCTAGGAATCGGACGATTAGCCATTATAAAATCAATAGGCTATCAGGAACATTTAACAGAGTATGGAG TTCACTGGAACTTTTTCTTTACCATAATAGTTGTGAAATTGATAACACCACTGCTGTTGATTATTTTTCC CCTAAATAAGTCCTGGATTATTGCCCTCGGCATTACTGTATTATACCAGCTAGCCCTTGACTTTACCTCA CTGAAGAGGTTAATATTATATGGCACTGATGGTAGTGGCACACGGGTTGGTCTATTAAATGCCAACCGCG AAGGAATAATCTCTACCCTGGGGTATGTGGCAATACACATGGCTGGTGTGCAAACAGGGTTATATATGCA TAAGAACCGATCACATATCAAAGACTTGATAAAAGTAGCCTGTTTTCTTTTACTGGCAGCTATTAGCCTC TTCATATCTCTTTACGTAGTTCAAGTAAATGTAGAAGCAGTATCTCGAAGAATGGCAAATTTAGCCTTTT GTATTTGGATAGTTGCTTCTAGCCTGATCCTTCTTAGTAGTTTATTACTGGGTGATATAATTTTGAGTTT TGCCAAATTTCTAATTAAAGGAGCTCTAGTACCATGTTCTTGGAAACTTATCCAGTCACCTGTTACAAAT AAAAAGCATTCAGAATCTCTAGTCCCTGAAGCCGAAAGAATGGAACCCAGTCTTTGTTTAATCACAGCTC TAAACAGAAAACAGTTAATATTTTTCTTGCTGTCAAATATAACAACTGGCCTGATCAACCTGATGGTAGA TACATTACACAGCAGTACCTTGTGGGCCTTATTTGTGGTCAATCTCTATATGTTTTCCAACTGTTTAATT GTATATGTACTATATTTGCAAGATAAGACTGTACAATTTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217028 representing NM_178517
Red=Cloning site Green=Tags(s) MSEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVA TLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPAISCFRVITSAFT AIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLV FLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTS LKRLILYGTDGSGTRVGLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISL FISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTN KKHSESLVPEAERMEPSLCLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLYMFSNCLI VYVLYLQDKTVQFW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178517 |
| ORF Size | 1512 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178517.4 |
| RefSeq Size | 3050 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 284098 |
| UniProt ID | Q7Z7B1 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 56.9 kDa |
| Gene Summary | The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217028L3 | Lenti-ORF clone of PIGW (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 6,370.00 |
|
| RC217028L4 | Lenti-ORF clone of PIGW (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 6,370.00 |
|
| RG217028 | PIGW (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW). Note: ORF is codon optimized |
CNY 4,940.00 |
|
| SC313764 | PIGW (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class W (PIGW) |
CNY 6,016.00 |
