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PTCH1 (NM_000264) Human Tagged ORF Clone
CAT＃: RC216999
- TrueORF®
PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b
ORF Plasmid: tGFP
"NM_000264" in other vectors (6)
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CNY 15,840.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCNS; NBCCS; PTC; PTC1; PTCH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216999 representing NM_000264
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGCTGGTAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGTATCGGTGCCC CGGGACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGCCGTGCTGCCGCGCCGGACCG GGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGCTCTGGAGCAGATTTCCAAGGGGAAGGCT ACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAGAGACTCTTATTTAAACTGGGTTGTTACA TTCAAAAAAACTGCGGCAAGTTCTTGGTTGTGGGCCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAA AGCAGCGAACCTCGAGACCAACGTGGAGGAGCTGTGGGTGGAAGTTGGAGGACGAGTAAGTCGTGAATTA AATTATACTCGCCAGAAGATTGGAGAAGAGGCTATGTTTAATCCTCAACTCATGATACAGACCCCTAAAG AAGAAGGTGCTAATGTCCTGACCACAGAAGCGCTCCTACAACACCTGGACTCGGCACTCCAGGCCAGCCG TGTCCATGTATACATGTACAACAGGCAGTGGAAATTGGAACATTTGTGTTACAAATCAGGAGAGCTTATC ACAGAAACAGGTTACATGGATCAGATAATAGAATATCTTTACCCTTGTTTGATTATTACACCTTTGGACT GCTTCTGGGAAGGGGCGAAATTACAGTCTGGGACAGCATACCTCCTAGGTAAACCTCCTTTGCGGTGGAC AAACTTCGACCCTTTGGAATTCCTGGAAGAGTTAAAGAAAATAAACTATCAAGTGGACAGCTGGGAGGAA ATGCTGAATAAGGCTGAGGTTGGTCATGGTTACATGGACCGCCCCTGCCTCAATCCGGCCGATCCAGACT GCCCCGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTGATATGGCCCTTGTTTTGAATGGTGGATG TCATGGCTTATCCAGAAAGTATATGCACTGGCAGGAGGAGTTGATTGTGGGTGGCACAGTCAAGAACAGC ACTGGAAAACTCGTCAGCGCCCATGCCCTGCAGACCATGTTCCAGTTAATGACTCCCAAGCAAATGTACG AGCACTTCAAGGGGTACGAGTATGTCTCACACATCAACTGGAACGAGGACAAAGCGGCAGCCATCCTGGA GGCCTGGCAGAGGACATATGTGGAGGTGGTTCATCAGAGTGTCGCACAGAACTCCACTCAAAAGGTGCTT TCCTTCACCACCACGACCCTGGACGACATCCTGAAATCCTTCTCTGACGTCAGTGTCATCCGCGTGGCCA GCGGCTACTTACTCATGCTCGCCTATGCCTGTCTAACCATGCTGCGCTGGGACTGCTCCAAGTCCCAGGG TGCCGTGGGGCTGGCTGGCGTCCTGCTGGTTGCACTGTCAGTGGCTGCAGGACTGGGCCTGTGCTCATTG ATCGGAATTTCCTTTAACGCTGCAACAACTCAGGTTTTGCCATTTCTCGCTCTTGGTGTTGGTGTGGATG ATGTTTTTCTTCTGGCCCACGCCTTCAGTGAAACAGGACAGAATAAAAGAATCCCTTTTGAGGACAGGAC CGGGGAGTGCCTGAAGCGCACAGGAGCCAGCGTGGCCCTCACGTCCATCAGCAATGTCACAGCCTTCTTC ATGGCCGCGTTAATCCCAATTCCCGCTCTGCGGGCGTTCTCCCTCCAGGCAGCGGTAGTAGTGGTGTTCA ATTTTGCCATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCATGGATTTATATCGACGCGAGGACAGGAG ACTGGATATTTTCTGCTGTTTTACAAGCCCCTGCGTCAGCAGAGTGATTCAGGTTGAACCTCAGGCCTAC ACCGACACACACGACAATACCCGCTACAGCCCCCCACCTCCCTACAGCAGCCACAGCTTTGCCCATGAAA CGCAGATTACCATGCAGTCCACTGTCCAGCTCCGCACGGAGTACGACCCCCACACGCACGTGTACTACAC CACCGCTGAGCCGCGCTCCGAGATCTCTGTGCAGCCCGTCACCGTGACACAGGACACCCTCAGCTGCCAG AGCCCAGAGAGCACCAGCTCCACAAGGGACCTGCTCTCCCAGTTCTCCGACTCCAGCCTCCACTGCCTCG AGCCCCCCTGTACGAAGTGGACACTCTCATCTTTTGCTGAGAAGCACTATGCTCCTTTCCTCTTGAAACC AAAAGCCAAGGTAGTGGTGATCTTCCTTTTTCTGGGCTTGCTGGGGGTCAGCCTTTATGGCACCACCCGA GTGAGAGACGGGCTGGACCTTACGGACATTGTACCTCGGGAAACCAGAGAATATGACTTTATTGCTGCAC AATTCAAATACTTTTCTTTCTACAACATGTATATAGTCACCCAGAAAGCAGACTACCCGAATATCCAGCA CTTACTTTACGACCTACACAGGAGTTTCAGTAACGTGAAGTATGTCATGTTGGAAGAAAACAAACAGCTT CCCAAAATGTGGCTGCACTACTTCAGAGACTGGCTTCAGGGACTTCAGGATGCATTTGACAGTGACTGGG AAACCGGGAAAATCATGCCAAACAATTACAAGAATGGATCAGACGATGGAGTCCTTGCCTACAAACTCCT GGTGCAAACCGGCAGCCGCGATAAGCCCATCGACATCAGCCAGTTGACTAAACAGCGTCTGGTGGATGCA GATGGCATCATTAATCCCAGCGCTTTCTACATCTACCTGACGGCTTGGGTCAGCAACGACCCCGTCGCGT ATGCTGCCTCCCAGGCCAACATCCGGCCACACCGACCAGAATGGGTCCACGACAAAGCCGACTACATGCC TGAAACAAGGCTGAGAATCCCGGCAGCAGAGCCCATCGAGTATGCCCAGTTCCCTTTCTACCTCAACGGC TTGCGGGACACCTCAGACTTTGTGGAGGCAATTGAAAAAGTAAGGACCATCTGCAGCAACTATACGAGCC TGGGGCTGTCCAGTTACCCCAACGGCTACCCCTTCCTCTTCTGGGAGCAGTACATCGGCCTCCGCCACTG GCTGCTGCTGTTCATCAGCGTGGTGTTGGCCTGCACATTCCTCGTGTGCGCTGTCTTCCTTCTGAACCCC TGGACGGCCGGGATCATTGTGATGGTCCTGGCGCTGATGACGGTCGAGCTGTTCGGCATGATGGGCCTCA TCGGAATCAAGCTCAGTGCCGTGCCCGTGGTCATCCTGATCGCTTCTGTTGGCATAGGAGTGGAGTTCAC CGTTCACGTTGCTTTGGCCTTTCTGACGGCCATCGGCGACAAGAACCGCAGGGCTGTGCTTGCCCTGGAG CACATGTTTGCACCCGTCCTGGATGGCGCCGTGTCCACTCTGCTGGGAGTGCTGATGCTGGCGGGATCTG AGTTCGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCACCATCCTCGGCGTTCTCAATGG GCTGGTTTTGCTTCCCGTGCTTTTGTCTTTCTTTGGACCATATCCTGAGGTGTCTCCAGCCAACGGCTTG AACCGCCTGCCCACACCCTCCCCTGAGCCACCCCCCAGCGTGGTCCGCTTCGCCATGCCGCCCGGCCACA CGCACAGCGGGTCTGATTCCTCCGACTCGGAGTATAGTTCCCAGACGACAGTGTCAGGCCTCAGCGAGGA GCTTCGGCACTACGAGGCCCAGCAGGGCGCGGGAGGCCCTGCCCACCAAGTGATCGTGGAAGCCACAGAA AACCCCGTCTTCGCCCACTCCACTGTGGTCCATCCCGAATCCAGGCATCACCCACCCTCGAACCCGAGAC AGCAGCCCCACCTGGACTCAGGGTCCCTGCCTCCCGGACGGCAAGGCCAGCAGCCCCGCAGGGACCCCCC CAGAGAAGGCTTGTGGCCACCCCCCTACAGACCGCGCAGAGACGCTTTTGAAATTTCTACTGAAGGGCAT TCTGGCCCTAGCAATAGGGCCCGCTGGGGCCCTCGCGGGGCCCGTTCTCACAACCCTCGGAACCCAGCGT CCACTGCCATGGGCAGCTCCGTGCCCGGCTACTGCCAGCCCATCACCACTGTGACGGCTTCTGCCTCCGT GACTGTCGCCGTGCACCCGCCGCCTGTCCCTGGGCCTGGGCGGAACCCCCGAGGGGGACTCTGCCCAGGC TACCCTGAGACTGACCACGGCCTGTTTGAGGACCCCCACGTGCCTTTCCACGTCCGGTGTGAGAGGAGGG ATTCGAAGGTGGAAGTCATTGAGCTGCAGGACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAGCTCCAA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216999 representing NM_000264
Red=Cloning site Green=Tags(s) MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKA TGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSREL NYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELI TETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEE MLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNS TGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVL SFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSL IGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFF MAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAY TDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQ SPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTR VRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQL PKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDA DGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNG LRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNP WTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALE HMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGL NRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATE NPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGH SGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPG YPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000264 |
| ORF Size | 4341 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000264.5 |
| RefSeq Size | 6825 bp |
| RefSeq ORF | 4344 bp |
| Locus ID | 5727 |
| UniProt ID | Q13635 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| MW | 160.4 kDa |
| Gene Summary | This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216999L1 | Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 18,240.00 |
|
| RC216999L2 | Lenti-ORF clone of PTCH1 (mGFP-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 18,240.00 |
|
| RC216999L3 | Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,680.00 |
|
| RC216999L4 | Lenti-ORF clone of PTCH1 (mGFP-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,680.00 |
|
| RG216999 | PTCH1 (tGFP-tagged) - Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,200.00 |
|
| SC300040 | PTCH1 (untagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 14,864.00 |
