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WAVE 1 (WASF1) (NM_003931) Human Tagged ORF Clone
CAT＃: RC216977
WASF1 (Myc-DDK-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1
ORF Plasmid: tGFP
"NM_003931" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NEDALVS; SCAR1; WAVE; WAVE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216977 representing NM_003931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCACACAGCACTGCCTAGAGGCATTAAGA ATGAACTGGAATGTGTAACCAATATTTCCTTGGCAAATATAATTAGACAACTAAGTAGCCTAAGTAAATA TGCTGAAGATATATTTGGAGAATTATTCAATGAAGCACATAGTTTTTCCTTCAGAGTCAACTCATTGCAA GAACGTGTGGACCGTTTATCTGTTAGTGTTACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAG ATATAACAATGAGGAAAGCTTTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAGACTTT GCCTATTCCATTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAATATACTCACTCCTTAT AGAGATGATGGTAAAGAAGGTCTGAAGTTTTATACCAATCCTTCGTATTTCTTTGATCTATGGAAAGAAA AAATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGGAAGCAGAAGCAGAAAAATCTAGATCGTCC TCATGAACCAGAAAAAGTGCCAAGAGCACCTCATGACAGGCGGCGAGAATGGCAGAAGCTGGCCCAAGGT CCAGAGCTGGCTGAAGATGATGCTAATCTCTTACATAAGCATATTGAAGTTGCTAATGGCCCAGCCTCTC ATTTTGAAACAAGACCTCAGACATACGTGGATCATATGGATGGATCTTACTCACTTTCTGCCTTGCCATT TAGTCAGATGAGTGAGCTTCTGACTAGAGCTGAGGAAAGGGTATTAGTCAGACCACATGAACCACCTCCA CCTCCACCAATGCATGGAGCAGGAGATGCAAAACCGATACCCACCTGTATCAGTTCTGCTACAGGTTTGA TAGAAAATCGCCCTCAGTCACCAGCTACAGGCAGAACACCTGTGTTTGTGAGCCCCACTCCCCCACCTCC TCCACCACCTCTTCCATCTGCCTTGTCAACTTCCTCATTAAGAGCTTCAATGACTTCAACTCCTCCCCCT CCAGTACCTCCCCCACCTCCACCTCCAGCCACTGCTTTGCAAGCTCCAGCAGTACCACCACCTCCAGCTC CTCTTCAGATTGCCCCTGGAGTTCTTCACCCAGCTCCTCCTCCAATTGCACCTCCTCTAGTACAGCCCTC TCCACCAGTAGCTAGAGCTGCCCCAGTATGTGAGACTGTACCAGTTCATCCACTCCCACAAGGTGAAGTT CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTGCCTCCACCTGGCATTCGACCATCATCACCTGTCA CAGTTACAGCTCTTGCTCATCCTCCCTCTGGGCTACATCCAACTCCATCTACTGCCCCAGGTCCCCATGT TCCATTAATGCCTCCATCTCCTCCATCACAAGTTATACCTGCTTCTGAGCCAAAGCGCCATCCATCAACC CTACCTGTAATCAGTGATGCCAGGAGTGTGCTACTGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAG TAGAAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCATCCTGTCTCG CCGTATTGCTGTTGAATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGATTGGTTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216977 representing NM_003931
Red=Cloning site Green=Tags(s) MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQ ERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPY RDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQG PELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPP PPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPP PVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEV QGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPST LPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003931 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003931.3 |
| RefSeq Size | 3230 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 8936 |
| UniProt ID | Q92558 |
| Domains | WH2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| MW | 61.5 kDa |
| Gene Summary | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216977L1 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216977L2 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216977L3 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216977L4 | Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216977 | WASF1 (tGFP-tagged) - Human WAS protein family, member 1 (WASF1), transcript variant 1 |
CNY 4,370.00 |
|
| SC117699 | WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1 |
CNY 2,950.00 |
