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CDC14A (NM_033312) Human Tagged ORF Clone
CAT＃: RC216931
CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 2
ORF Plasmid: tGFP
"NM_033312" in other vectors (4)
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CNY 4,640.00
CNY 7,315.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | cdc14; DFNB32; DFNB35; DFNB105; hCDC14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216931 representing NM_033312
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTACTT TAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGCTGGTCTATGAAAA TTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGATATTGCTGCAAACTAAACAAGAAA CTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGCACTACACCTGTTTTGACCAACGGAAAAGAGCAA ATGCAGCATTTTTGATAGGTGCCTATGCAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGC ACTCCTGTCTGGCTCAAACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAAT CTCACCATTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGACAT TTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGATTGTTCCAGGAAA ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATGGTTATCCTCTTCACGCCCCTGAA GCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTGCAGTTGTGAGGCTAAACAAAAAGATTTATGAGG CAAAGCGCTTCACAGACGCTGGCTTCGAGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGA CAACATCGTGCGAAGGTTCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCT GGTCTTGGAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTGAAA TAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACTTCCTGGAAGAAAA ACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGAAAAATCGACCATCCAGTGAAGGA AGTATTAATAAAATTCTTTCTGGCCTAGATGATATGTCTATTGGTGGAAATCTTTCAAAAACACAAAACA TGGAACGATTTGGAGAGGATAACTTAGAAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGA CAAACTACGTGCCTTAAAAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGAT ACAAAAGGACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAGTTA CTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACAGAACTTCTTTGTC TTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAATGCT GCAACAGATGATCCAGAGAACAAAAAGACCTCCTCATCCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTA CCAACCTCTTGAATGGCAGCTCCCAGCCAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAA CAGAAGCAGCAACAGCAACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAG CACACCACCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTTCTA TCCCTGTAAGTGCGCAGACACCACCTCCTGGTCCTCAGAACCCTGAATGCAACTTCTGTGCCTTGCCTTC CCAGCCGAGGCTGCCACCAAAGAAATTTAATAGTGCCAAGGAAGCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216931 representing NM_033312
Red=Cloning site Green=Tags(s) MAAESGELIGACEFMKDRLYFATLRNRPKSTVNTHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK LKSYSLSRKKIVHYTCFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYN LTILDCLQGIRKGLQHGFFDFETFDVDEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPE AYFPYFKKHNVTAVVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKA GLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNRPSSEG SINKILSGLDDMSIGGNLSKTQNMERFGEDNLEDDDVEMKNGITQGDKLRALKSQRQPRTSPSCAFRSDD TKGHPRAVSQPFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNA ATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPELNNNQYNRSSNSNGGNLNSPPGPHSAKTEE HTTILRPSYTGLSSSSARFLSRSIPVSAQTPPPGPQNPECNFCALPSQPRLPPKKFNSAKEAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033312 |
| ORF Size | 1869 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033312.2, NP_201569.1 |
| RefSeq Size | 2438 bp |
| RefSeq ORF | 1872 bp |
| Locus ID | 8556 |
| UniProt ID | Q9UNH5 |
| Domains | Y_phosphatase |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Cell cycle |
| MW | 69.4 kDa |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216931L3 | Lenti-ORF clone of CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 2 |
CNY 5,890.00 |
|
| RC216931L4 | Lenti-ORF clone of CDC14A (mGFP-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 2 |
CNY 5,890.00 |
|
| RG216931 | CDC14A (tGFP-tagged) - Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 2 |
CNY 4,370.00 |
|
| SC314240 | CDC14A (untagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 2 |
CNY 10,070.00 |
