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ARHGEF4 (NM_032995) Human Tagged ORF Clone
CAT＃: RC216845
ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2
ORF Plasmid: tGFP
"NM_032995" in other vectors (4)
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CNY 4,912.00
CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASEF; ASEF1; GEF4; STM6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216845 representing NM_032995
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAGC CTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACTGT GCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCTGTGG CTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGGGCAG TGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAG AAGAAGCCATCCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCA GGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACC TGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGCTAT CAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGTCACCATGGACGAC CAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGG GCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCC CGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGC AAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGC GCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCTGCG TACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCAGAGG TTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGA TCTACTCGGAGTACTGCAATAACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAA GTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTG CTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCC AGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAACGA GCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGAGGACTGGGAGGGA GAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAG CCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCG CCGCGACGTGTTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGG AAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCC ACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGAGCA GGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTGCAGAGGAAGCAGGCCATGCTGAATGCC AGCAAGCAGCAGGTCACAGGGAAGCCCAAAGGTAGGCGGACAGCAGCCCCACCTCCTCGGCTGCCCGGTC CTTACCCTGCTGACATCATTCCCTTCTCTGAACCACAAAGCCAAGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216845 representing NM_032995
Red=Cloning site Green=Tags(s) MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLW LEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTP GTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDD QELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSS KDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQR FNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEG EDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDG KDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNA SKQQVTGKPKGRRTAAPPPRLPGPYPADIIPFSEPQSQAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032995 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032995.2, NP_127462.1 |
| RefSeq Size | 4258 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 50649 |
| UniProt ID | Q9NR80 |
| Protein Families | Druggable Genome |
| Protein Pathways | Regulation of actin cytoskeleton |
| MW | 76.2 kDa |
| Gene Summary | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216845L3 | Lenti-ORF clone of ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 9,030.00 |
|
| RC216845L4 | Lenti-ORF clone of ARHGEF4 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 9,030.00 |
|
| RG216845 | ARHGEF4 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 7,890.00 |
|
| SC305530 | ARHGEF4 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 |
CNY 13,020.00 |
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