Collagen XI alpha 2 (COL11A2) (NM_080679) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216569 representing NM_080679 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTGCAGCCGCTGCCATCGCCTCCTCCTCCTCCTACCTCTGGTGCTGGGGCTGAGCGCGGCCC CAGGCTGGGCAGGTGCACCCCCTGTGGATGTGCTCCGGGCCCTGAGGTTCCCCTCCCTCCCTGATGGTGT CCGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTACCGAGTGGCACGACCTGCCCAGCTCAGTGCA CCCACTCGCCAGCTTTTCCCAGGAGGATTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCC CTGGTCTCCAAGCTCCCCTCCTGACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTGGAGCTGGG CCGACCTGTCCGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCTGTGGCTGTGAAGGGCCAGTCTGTCACCCTCA TTGTTGACTGCAAGAAGCGAGTCACCCGGCCTCTCCCCCGAAGTGCTCGTCCAGTATTGGACACCCATGG AGTGATCATCTTTGGTGCCCGTATTCTGGATGAAGAAGTCTTTGAGGGTGATGTCCAGGAGCTGGCCATT GTCCCAGGGGTCCAGGCAGCCTATGAATCATGTGAACAGAAGGAGCTGGAATGCGAGGGGGGCCAGAGGG AAAGACCCCAAAACCAACAGCCTCACAGAGCCCAGAGATCTCCACAGCAGCAACCATCAAGACTTCACAG GCCACAAAATCAGGAACCCCAGAGCCAGGCTGCCCATGGACCCCGAGGGCTGAAGGGAGAGAAAGGAGAG CCTGCAGTGTTGGAACCTGGTATGCTCGTGGAGGGGCCCCCTGGCCCAGAAGGCCCTGCGGGATTGATTG GTCCCCCTGGCATCCAGGGGAACCCAGGCCCAGTTGGAGACCCTGGAGAGAGGGGCCCCCCTGGCCGAGC AGGGCTCCCTGGATCAGATGGGGCTCCTGGTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGC AGTGGTGGGGGTGACAAGGGCCCTGTGGTGGCGGCCCAGGAGGCTCAGGCCCAGGCGATCCTGCAGCAGG CGAGGCTGGCGCTCCGTGGACCCCCTGGCCCCATGGGATACACAGGGCGCCCTGGACCCTTGGGCCAACC TGGGAGCCCTGGCCTGAAAGGAGAGTCTGGAGACTTAGGACCTCAGGGCCCCAGAGGACCTCAGGGCCTC ACAGGCCCTCCTGGCAAGGCTGGGCGAAGGGGCCGGGCAGGTGCTGATGGAGCCCGAGGGATGCCTGGAG ATCCTGGAGTGAAGGGTGACCGAGGTTTTGATGGACTCCCAGGGCTCCCTGGAGAGAAGGGCCATAGGGG TGATACTGGTGCCCAGGGCCTTCCTGGTCCCCCTGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATT GGGCCTCGAGGGCTGCCTGGAGAGTCGGGACCTCGAGGTCTCCTTGGCCCCAAAGGCCCACCTGGTATTC CTGGACCCCCTGGCGTCCGAGGCATGGATGGTCCCCAGGGCCCCAAAGGGAGCTTGGGACCCCAGGGAGA GCCAGGACCTCCTGGACAACAGGGCACCCCTGGGACCCAGGGTCTTCCCGGGCCCCAGGGTGCCATCGGC CCTCATGGAGAGAAGGGTCCTCAAGGGAAGCCAGGGCTCCCCGGCATGCCTGGCTCAGACGGACCCCCGG GTCACCCAGGGAAGGAAGGTCCCCCTGGAACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCTCT AGGATACCCAGGACCTCGAGGGGTCAAGGGTGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGTGAG AAGGGTGAGGATGGCTTTCCTGGGTTCAAAGGTGACATAGGCGTGAAAGGTGACAGGGGCGAAGTTGGAG TCCCTGGTTCCAGGGGAGAGGATGGTCCTGAGGGGCCAAAGGGACGCACTGGACCGACTGGAGACCCTGG GCCCCCAGGGCTCATGGGCGAGAAGGGCAAGCTGGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGTCAG GGACCCAAGGGGTCCCTAGGATTTCCTGGCTTTCCTGGTGCCAGTGGAGAGAAGGGAGCCCGGGGCCTGT CGGGGAAGTCAGGGCCTCGGGGAGAACGGGGCCCCACGGGTCCACGGGGTCAGCGGGGACCCCGAGGTGC CACTGGGAAGTCTGGAGCTAAGGGAACATCTGGTGGTGATGGCCCCCATGGGCCCCCTGGAGAGAGGGGC CTCCCTGGACCTCAGGGTCCCAACGGGTTTCCTGGACCGAAAGGACCCCCGGGCCCCCCTGGGAAGGATG GGCTGCCGGGACACCCAGGCCAAAGAGGAGAAGTGGGTTTCCAAGGGAAGACCGGCCCCCCTGGTCCTCC AGGAGTGGTGGGACCTCAGGGAGCAGCAGGAGAAACCGGCCCTATGGGGGAGAGAGGTCACCCAGGCCCC CCGGGGCCCCCTGGAGAGCAGGGACTACCTGGGACAGCTGGAAAAGAAGGAACAAAGGGTGACCCTGGTC CCCCTGGGGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGGGGATTCCCAGGAGAGAGAGGCCTCCCAGG CACTGCTGGTGGACCTGGTTTGAAGGGGAATGAAGGTCCGTCTGGCCCCCCTGGCCCTGCAGGCTCCCCT GGGGAACGAGGTGCAGCAGGATCAGGGGGACCCATTGGTCCGCCAGGGCGCCCAGGCCCGCAGGGTCCCC CTGGAGCAGCAGGAGAGAAAGGTGTCCCAGGTGAGAAGGGCCCCATTGGCCCGACTGGCCGAGATGGAGT GCAGGGTCCTGTGGGGCTTCCTGGTCCTGCTGGGCCTCCAGGTGTGGCTGGAGAGGATGGAGACAAGGGT GAGGTGGGGGACCCCGGACAGAAGGGCACCAAAGGGAACAAGGGTGAACATGGCCCTCCTGGACCCCCTG GACCCATTGGTCCTGTGGGGCAGCCTGGAGCAGCGGGAGCAGATGGGGAGCCCGGAGCTCGGGGACCCCA GGGACACTTTGGAGCCAAAGGTGATGAAGGAACAAGAGGATTCAATGGGCCCCCAGGACCCATTGGCCTA CAGGGTTTGCCAGGCCCCTCTGGGGAGAAGGGAGAAACAGGAGATGTGGGTCCTATGGGACCACCTGGCC CCCCAGGACCTCGAGGTCCAGCTGGACCCAATGGCGCTGATGGCCCACAAGGTCCCCCAGGAGGTGTTGG GAACCTGGGTCCCCCTGGAGAGAAGGGGGAACCAGGAGAGTCAGGATCTCCAGGGATCCAGGGCGAGCCA GGTGTCAAGGGTCCACGCGGGGAACGTGGAGAGAAAGGAGAGTCGGGGCAGCCAGGAGAGCCAGGGCCAC CAGGGCCTAAAGGCCCCACAGGCGATGATGGCCCCAAAGGGAACCCTGGTCCTGTTGGTTTTCCTGGTGA CCCTGGCCCCCCTGGAGAAGGTGGCCCTCGGGGCCAGGATGGTGCTAAGGGTGACCGAGGCGAGGATGGT GAGCCAGGACAGCCTGGATCCCCTGGTCCCACCGGGGAGAATGGACCCCCAGGGCCACTTGGAAAGCGAG GTCCTGCTGGCTCGCCTGGTTCCGAGGGGCGACAAGGAGGGAAGGGAGCCAAGGGAGATCCTGGCGCTAT AGGTGCCCCGGGGAAGACAGGCCCGGTGGGTCCTGCAGGCCCAGCAGGGAAACCTGGCCCTGATGGTCTG AGGGGGCTCCCAGGCTCAGTGGGTCAGCAAGGCCGACCTGGAGCTACAGGCCAGGCTGGGCCCCCAGGTC CTGTGGGACCCCCAGGGCTGCCTGGTCTCCGGGGCGATGCTGGAGCCAAGGGAGAGAAGGGCCACCCAGG TCTCATTGGACTGATTGGGCCCCCGGGTGAGCAGGGAGAGAAGGGAGATCGGGGACTTCCTGGGCCTCAG GGCTCCCCTGGGCAGAAGGGTGAGATGGGTATCCCAGGAGCATCCGGCCCCATTGGTCCTGGAGGTCCCC CCGGCCTCCCCGGACCTGCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGGCGGACCCAAGGGAGA GAAGGGTGTGCAGGGCCCTCCAGGACACCCGGGTCCCCCAGGCGAGGTGATCCAGCCACTGCCCATTCAG ATGCCCAAGAAGACTCGGCGCTCGGTGGATGGAAGCCGTCTGATGCAGGAAGATGAGGCCATACCGACCG GGGGAGCCCCCGGCAGTCCTGGGGGGCTGGAGGAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGAT CGAGCAGATGAGGCGGCCAACAGGGACCCAGGACAGCCCTGCTCGCACCTGCCAGGACCTGAAGCTGTGC CACCCAGAGCTTCCCGATGGAGAGTACTGGGTCGACCCCAACCAGGGCTGTGCTCGGGATGCCTTCCGAG TTTTCTGCAACTTCACAGCAGGGGGTGAGACCTGTGTGACGCCTAGGGATGACGTCACGCAGTTCTCTTA CGTGGACTCAGAGGGCTCCCCAGTGGGTGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCAGCC CACCAGGACGTCTCCTACCCCTGCTCTGGAGCAGCCCGTGACGGTCCCCTGAGACTCCGTGGGGCCAATG AGGATGAGCTGAGCCCGGAGACTAGCCCCTATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGG CCGGACGGTGCTGGAGGTGCGAACGCCTGTGCTGGAGCAGCTGCCAGTGCTGGATGCCTCCTTCTCAGAC CTGGGAGCCCCACCGAGGCGGGGAGGGGTGCTGCTGGGGCCTGTCTGCTTCATGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216569 representing NM_080679 Red=Cloning site Green=Tags(s) MERCSRCHRLLLLLPLVLGLSAAPGWAGAPPVDVLRALRFPSLPDGVRRAKGICPADVAYRVARPAQLSA PTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVRQLGLELGRPVRFLYEDQTGRPQPPSQPVFR GLSLADGKWHRVAVAVKGQSVTLIVDCKKRVTRPLPRSARPVLDTHGVIIFGARILDEEVFEGDVQELAI VPGVQAAYESCEQKELECEGGQRERPQNQQPHRAQRSPQQQPSRLHRPQNQEPQSQAAHGPRGLKGEKGE PAVLEPGMLVEGPPGPEGPAGLIGPPGIQGNPGPVGDPGERGPPGRAGLPGSDGAPGPPGTSLMLPFRFG SGGGDKGPVVAAQEAQAQAILQQARLALRGPPGPMGYTGRPGPLGQPGSPGLKGESGDLGPQGPRGPQGL TGPPGKAGRRGRAGADGARGMPGDPGVKGDRGFDGLPGLPGEKGHRGDTGAQGLPGPPGEDGERGDDGEI GPRGLPGESGPRGLLGPKGPPGIPGPPGVRGMDGPQGPKGSLGPQGEPGPPGQQGTPGTQGLPGPQGAIG PHGEKGPQGKPGLPGMPGSDGPPGHPGKEGPPGTKGNQGPSGPQGPLGYPGPRGVKGVDGIRGLKGHKGE KGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPTGDPGPPGLMGEKGKLGVPGLPGYPGRQ GPKGSLGFPGFPGASGEKGARGLSGKSGPRGERGPTGPRGQRGPRGATGKSGAKGTSGGDGPHGPPGERG LPGPQGPNGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKTGPPGPPGVVGPQGAAGETGPMGERGHPGP PGPPGEQGLPGTAGKEGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTAGGPGLKGNEGPSGPPGPAGSP GERGAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQGPVGLPGPAGPPGVAGEDGDKG EVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGHFGAKGDEGTRGFNGPPGPIGL QGLPGPSGEKGETGDVGPMGPPGPPGPRGPAGPNGADGPQGPPGGVGNLGPPGEKGEPGESGSPGIQGEP GVKGPRGERGEKGESGQPGEPGPPGPKGPTGDDGPKGNPGPVGFPGDPGPPGEGGPRGQDGAKGDRGEDG EPGQPGSPGPTGENGPPGPLGKRGPAGSPGSEGRQGGKGAKGDPGAIGAPGKTGPVGPAGPAGKPGPDGL RGLPGSVGQQGRPGATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQ GSPGQKGEMGIPGASGPIGPGGPPGLPGPAGPKGAKGATGPGGPKGEKGVQGPPGHPGPPGEVIQPLPIQ MPKKTRRSVDGSRLMQEDEAIPTGGAPGSPGGLEEIFGSLDSLREEIEQMRRPTGTQDSPARTCQDLKLC HPELPDGEYWVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVTQFSYVDSEGSPVGVVQLTFLRLLSVSA HQDVSYPCSGAARDGPLRLRGANEDELSPETSPYVKEFRDGCQTQQGRTVLEVRTPVLEQLPVLDASFSD LGAPPRRGGVLLGPVCFMG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_080679
ORF Size	4887 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_080679.2, NP_542410.2
RefSeq Size	6104 bp
RefSeq ORF	4890 bp
Locus ID	1302
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	159.9 kDa
Gene Summary	This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]

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