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HPSE2 (NM_021828) Human Tagged ORF Clone
CAT＃: RC216534
HPSE2 (Myc-DDK-tagged)-Human heparanase 2 (HPSE2), transcript variant 1
ORF Plasmid: tGFP
"NM_021828" in other vectors (4)
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CNY 6,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HPA2; HPR2; UFS; UFS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216534 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTGCTTTGTGCCTTCCCTGAAGCCATGCCCTCCAGCAACTCCCGCCCCCCCGCGTGCCTAGCCC CGGGGGCTCTCTACTTGGCTCTGTTGCTCCATCTCTCCCTTTCCTCCCAGGCTGGAGACAGGAGACCCTT GCCTGTAGACAGAGCTGCAGGTTTGAAGGAAAAGACCCTGATTCTACTTGATGTGAGCACCAAGAACCCA GTCAGGACAGTCAATGAGAACTTCCTCTCTCTGCAGCTGGATCCGTCCATCATTCATGATGGCTGGCTCG ATTTCCTAAGCTCCAAGCGCTTGGTGACCCTGGCCCGGGGACTTTCGCCCGCCTTTCTGCGCTTCGGGGG CAAAAGGACCGACTTCCTGCAGTTCCAGAACCTGAGGAACCCGGCGAAAAGCCGCGGGGGCCCGGGCCCG GATTACTATCTCAAAAACTATGAGGATGACATTGTTCGAAGTGATGTTGCCTTAGATAAACAGAAAGGCT GCAAGATTGCCCAGCACCCTGATGTTATGCTGGAGCTCCAAAGGGAGAAGGCAGCTCAGATGCATCTGGT TCTTCTAAAGGAGCAATTCTCCAATACTTACAGTAATCTCATATTAACAGCCAGGTCTCTAGACAAACTT TATAACTTTGCTGATTGCTCTGGACTCCACCTGATATTTGCTCTAAATGCACTGCGTCGTAATCCCAATA ACTCCTGGAACAGTTCTAGTGCCCTGAGTCTGTTGAAGTACAGCGCCAGCAAAAAGTACAACATTTCTTG GGAACTGGGTAATGAGCCAAATAACTATCGGACCATGCATGGCCGGGCAGTAAATGGCAGCCAGTTGGGA AAGGATTACATCCAGCTGAAGAGCCTGTTGCAGCCCATCCGGATTTATTCCAGAGCCAGCTTATATGGCC CTAATATTGGGCGGCCGAGGAAGAATGTCATCGCCCTCCTAGATGGATTCATGAAGGTGGCAGGAAGTAC AGTAGATGCAGTTACCTGGCAACATTGCTACATTGATGGCCGGGTGGTCAAGGTGATGGACTTCCTGAAA ACTCGCCTGTTAGACACACTCTCTGACCAGATTAGGAAAATTCAGAAAGTGGTTAATACATACACTCCAG GAAAGAAGATTTGGCTTGAAGGTGTGGTGACCACCTCAGCTGGAGGCACAAACAATCTATCCGATTCCTA TGCTGCAGGATTCTTATGGTTGAACACTTTAGGAATGCTGGCCAATCAGGGCATTGATGTCGTGATACGG CACTCATTTTTTGACCATGGATACAATCACCTCGTGGACCAGAATTTTAACCCATTACCAGACTACTGGC TCTCTCTCCTCTACAAGCGCCTGATCGGCCCCAAAGTCTTGGCTGTGCATGTGGCTGGGCTCCAGCGGAA GCCACGGCCTGGCCGAGTGATCCGGGACAAACTAAGGATTTATGCTCACTGCACAAACCACCACAACCAC AACTACGTTCGTGGGTCCATTACACTTTTTATCATCAACTTGCATCGATCAAGAAAGAAAATCAAGCTGA CTGGGACTCTCAGAGACAAGCTGGTTCACCAGTACCTGCTGCAGCCCTATGGGCAGGAGGGCCTAAAGTC CAAGTCAGTGCAACTGAATGGCCAGCCCTTAGTGATGGTGGACGACGGGACCCTCCCAGAATTGAAGCCC CGCCCCCTTCGGGCCGGCCGGACATTGGTCATCCCTCCAGTCACCATGGGCTTTTTTGTGGTCAAGAATG TCAATGCTTTGGCCTGCCGCTACCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216534 protein sequence
Red=Cloning site Green=Tags(s) MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDRRPLPVDRAAGLKEKTLILLDVSTKNP VRTVNENFLSLQLDPSIIHDGWLDFLSSKRLVTLARGLSPAFLRFGGKRTDFLQFQNLRNPAKSRGGPGP DYYLKNYEDDIVRSDVALDKQKGCKIAQHPDVMLELQREKAAQMHLVLLKEQFSNTYSNLILTARSLDKL YNFADCSGLHLIFALNALRRNPNNSWNSSSALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNGSQLG KDYIQLKSLLQPIRIYSRASLYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQHCYIDGRVVKVMDFLK TRLLDTLSDQIRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNNLSDSYAAGFLWLNTLGMLANQGIDVVIR HSFFDHGYNHLVDQNFNPLPDYWLSLLYKRLIGPKVLAVHVAGLQRKPRPGRVIRDKLRIYAHCTNHHNH NYVRGSITLFIINLHRSRKKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSVQLNGQPLVMVDDGTLPELKP RPLRAGRTLVIPPVTMGFFVVKNVNALACRYR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021828 |
| ORF Size | 1776 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021828.5 |
| RefSeq Size | 4349 bp |
| RefSeq ORF | 1779 bp |
| Locus ID | 60495 |
| UniProt ID | Q8WWQ2 |
| Protein Pathways | Glycosaminoglycan degradation, Metabolic pathways |
| MW | 66.6 kDa |
| Gene Summary | This gene encodes a heparanase enzyme. The encoded protein is a endoglycosidase that degrades heparin sulfate proteoglycans located on the extracellular matrix and cell surface. This protein may be involved in biological processes involving remodeling of the extracellular matrix including angiogenesis and tumor progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216534L3 | Lenti ORF clone of Human heparanase 2 (HPSE2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216534L4 | Lenti ORF clone of Human heparanase 2 (HPSE2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216534 | HPSE2 (tGFP-tagged) - Human heparanase 2 (HPSE2), transcript variant 1 |
CNY 4,370.00 |
|
| SC318019 | HPSE2 (untagged)-Human heparanase 2 (HPSE2), transcript variant 1 |
CNY 6,624.00 |
