Apc1 (ANAPC1) (NM_022662) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	APC1; MCPR; TSG24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216524 representing NM_022662 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAACTTCTATGAAGAAAGGACAACGATGATTGCAGCAAGGGATTTGCAGGAATTTGTTCCTTTTG GTCGAGACCACTGCAAGCACCACCCTAATGCTTTGAACCTTCAACTTCGCCAGCTGCAGCCAGCTTCTGA ATTATGGTCTTCTGATGGTGCTGCTGGCTTGGTGGGATCCCTTCAGGAGGTTACAATCCACGAGAAACAG AAGGAAAGCTGGCAGTTAAGGAAAGGAGTAAGTGAAATTGGAGAAGATGTGGACTATGATGAGGAACTCT ATGTTGCTGGAAATATGGTGATATGGAGCAAAGGAAGTAAAAGCCAGGCATTGGCAGTTTATAAAGCATT TACAGTTGACAGTCCTGTTCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG GCCTACAGTAGCAATGAAGTAGAAAAATGCATATGTATATTGCAAAGCTCATGTATTAACATGCATAGCA TAGAAGGAAAGGATTACATAGCTTCATTACCATTTCAGGTTGCAAATGTTTGGCCCACTAAATATGGATT GCTGTTTGAACGAAGCGCTTCTTCACATGAAGTACCTCCAGGTTCACCCAGAGAACCTTTACCTACTATG TTCAGCATGCTGCACCCACTAGATGAAATAACTCCACTTGTTTGTAAATCTGGAAGTCTTTTTGGTTCAT CACGGGTGCAATATGTTGTAGATCATGCAATGAAAATTGTTTTCCTCAATACTGACCCCTCTATTGTAAT GACTTATGATGCTGTTCAAAATGTGCATTCTGTGTGGACTCTCCGGAGAGTCAAATCAGAGGAAGAGAAT GTTGTTTTAAAGTTCTCTGAACAGGGGGGAACCCCACAGAATGTGGCCACTAGCAGCTCCCTCACAGCAC ATCTCAGAAGCCTCTCCAAAGGAGATTCCCCTGTGACTTCACCTTTCCAGAATTACTCCTCCATTCACAG CCAGAGTCGCTCAACCTCATCACCCAGTCTACATTCTCGCTCACCTTCTATTTCCAACATGGCAGCTCTA AGTCGTGCTCATTCTCCTGCGTTAGGAGTGCACTCTTTTTCAGGGGTGCAAAGGTTCAACATTTCAAGCC ATAATCAGTCTCCAAAGAGACATAGTATTTCTCATTCTCCAAATAGTAATTCTAATGGCTCCTTTCTTGC ACCAGAAACGGAGCCAATTGTTCCTGAACTGTGTATTGACCATTTGTGGACAGAAACGATTACTAATATA AGAGAGAAAAATTCACAAGCCTCAAAAGTGTTTATTACATCTGACCTATGTGGGCAAAAGTTCCTGTGCT TTTTAGTAGAGTCCCAGCTCCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGATAAAACCCAGCTCAT CTTTGGTTCAGTGACCAACATACCAGCAAAGGATGCAGCACCAGTGGAGAAAATAGACACCATGCTGGTC TTGGAAGGCAGTGGAAACCTGGTGCTATACACAGGAGTGGTTCGGGTGGGAAAGGTTTTTATTCCTGGAC TGCCAGCTCCCTCTCTGACGATGTCCAACACAATGCCTCGGCCCAGTACTCCACTAGATGGCGTTAGTAC TCCAAAGCCTCTTAGTAAACTCCTTGGATCATTGGACGAGGTTGTTCTGTTGTCCCCAGTTCCAGAACTG AGGGATTCTTCAAAACTTCATGATTCTCTCTATAATGAGGATTGTACTTTCCAACAGCTTGGAACTTACA TTCATTCTATCAGAGATCCTGTCCATAACAGAGTCACCCTGGAACTGAGTAATGGCTCCATGGTTAGGAT CACTATTCCTGAAATTGCCACCTCTGAGTTAGTACAAACGTGTTTGCAAGCAATTAAGTTTATCCTGCCA AAAGAAATAGCAGTTCAGATGCTTGTCAAGTGGTACAATGTCCACAGTGCTCCAGGAGGACCCAGTTATC ACTCAGAGTGGAATTTATTTGTGACTTGTCTCATGAACATGATGGGTTATAACACAGACCGCTTAGCATG GACTAGAAATTTTGACTTTGAAGGATCACTTTCTCCTGTCATTGCGCCCAAAAAAGCAAGGCCTTCCGAG ACTGGATCTGATGATGACTGGGAATATTTACTAAATTCAGACTACCACCAGAATGTTGAGTCTCATCTTT TGAACAGATCTTTATGTCTGAGTCCTTCAGAAGCTTCACAGATGAAGGATGAGGATTTTTCACAGAATCT CAGTCTGGATTCTTCTACACTTCTCTTTACTCACATACCTGCAATTTTTTTCGTTCTTCACCTTGTGTAT GAGGAGCTTAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCACTTGTTGAACTTCTCGTTCAGTTGG CAAGGGACTTAAAATTGGGGCCTTATGTAGATCATTACTATAGAGACTACCCAACGCTTGTCAGAACTAC TGGACAAGTGTGCACAATTGATCCAGGTCAAACAGGATTTATGCATCATCCATCATTTTTTACGTCTGAG CCACCAAGTATTTATCAGTGGGTGAGTTCTTGTCTGAAGGGTGAAGGAATGCCACCTTATCCTTACCTCC CTGGAATCTGTGAAAGAAGCAGACTTGTAGTCTTGAGTATTGCACTGTACATACTTGGTGATGAGAGCTT GGTTTCTGATGAATCCTCACAGTATTTAACCAGAATAACTATAGCCCCCCAGAAGTTGCAAGTAGAACAA GAGGAAAACAGGTTTAGTTTCAGGCATTCTACATCTGTTTCTAGTCTAGCTGAAAGATTGGTTGTCTGGA TGACTAATGTAGGATTCACTTTAAGAGATTTGGAAACTCTTCCCTTTGGAATTGCTCTTCCCATCAGAGA TGCAATTTATCACTGTCGTGAGCAGCCTGCCTCAGACTGGCCAGAAGCTGTCTGTCTCTTGATTGGACGT CAGGATCTTTCCAAGCAGGCCTGCGAAGGAAACTTACCCAAAGGGAAGTCTGTGCTCTCATCAGATGTTC CTTCAGGAACAGAAACTGAGGAGGAAGATGACGGCATGAATGACATGAATCACGAGGTCATGTCATTAAT ATGGAGTGAAGATTTAAGGGTGCAGGATGTGCGAAGGCTTCTTCAGAGTGCGCATCCTGTCCGTGTCAAC GTAGTGCAGTACCCAGAGCTCAGTGACCACGAGTTCATCGAGGAAAAGGAAAACAGATTGCTCCAATTGT GTCAGCGAACTATGGCTCTTCCTGTAGGACGAGGAATGTTTACCTTGTTTTCGTACCATCCTGTTCCAAC AGAGCCATTGCCTATTCCTAAATTGAATCTGACTGGGCGTGCCCCTCCTCGGAACACAACAGTAGACCTT AATAGTGGAAACATCGATGTGCCTCCCAACATGACAAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCTG GCCTGAAGATAGCTCCTGCCTCCCAGATCGACTCAGCTTGGATTGTTTACAATAAGCCCAAGCATGCTGA GTTGGCCAATGAGTATGCTGGCTTTCTCATGGCTCTGGGTTTGAATGGGCACCTTACCAAGCTGGCGACT CTCAATATCCATGACTACTTGACCAAGGGCCATGAAATGACAAGCATTGGACTGCTACTTGGTGTTTCTG CTGCAAAACTAGGCACCATGGATATGTCTATTACTCGGCTTCTTAGCATTCACATTCCTGCTCTCTTACC CCCAACGTCCACAGAGCTGGATGTTCCTCACAATGTCCAAGTGGCTGCAGTGGTTGGCATTGGCCTTGTA TATCAAGGGACAGCTCACAGACATACTGCAGAAGTCCTGTTGGCTGAGATAGGACGGCCTCCTGGTCCTG AAATGGAATACTGCACTGACAGAGAGTCATACTCCTTAGCTGCTGGCTTGGCCCTGGGCATGGTCTGCTT GGGGCATGGCAGCAATTTGATAGGTATGTCTGATCTCAATGTGCCTGAGCAGCTCTATCAGTACATGGTT GGAGGACATAGGCGCTTTCAAACAGGAATGCATAGGGAGAAACATAAATCACCAAGTTATCAAATCAAAG AAGGAGATACCATAAATGTGGATGTGACTTGTCCAGGTGCTACTCTAGCTTTGGCTATGATCTACTTAAA AACCAATAACAGATCTATTGCAGATTGGCTCCGAGCCCCTGACACCATGTATTTGTTGGACTTTGTGAAG CCAGAATTTCTCTTGCTTAGGACACTTGCTCGATGCCTGATTTTGTGGGATGATATTTTACCAAATTCCA AGTGGGTTGACAGCAATGTTCCTCAAATTATAAGAGAAAATAGTATCTCTCTCAGTGAAATCGAATTGCC GTGCTCAGAGGATTTGAATTTGGAAACTTTGTCCCAAGCACATGTCTACATAATTGCAGGAGCCTGCTTG TCTCTGGGTTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAACTGTTTGCATAAATTTGCCAAAG ATTTTATGACTTATTTGTCCGCACCTAATGCTTCTGTTACAGGTCCTCATAACCTAGAAACTTGTCTGAG CGTGGTGCTGCTGTCTCTCGCCATGGTCATGGCTGGCTCAGGAAACCTAAAGGTTTTGCAGCTTTGTCGC TTCTTACACATGAAAACGGGTGGTGAAATGAACTATGGTTTTCACTTAGCCCACCACATGGCCCTTGGAC TTCTATTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCAAATTCTTCCATTGCCGCTCTTCTCTGTGC CCTTTATCCGCACTTCCCAGCTCACAGCACTGACAACCGGTATCATCTCCAGGCTCTCCGGCACCTCTAT GTGCTGGCCGCGGAGCCCAGGCTTCTAGTGCCTGTGGATGTGGACACAAACACGCCCTGCTATGCCCTCT TAGAAGTTACCTACAAGGGCACTCAGTGGTATGAACAAACCAAAGAAGAATTGATGGCTCCTACCCTTCT TCCAGAACTCCATCTTTTAAAGCAGATTAAAGTAAAAGGCCCAAGATACTGGGAACTGCTCATAGATTTA AGCAAAGGAACACAACACTTGAAGTCCATCCTTTCCAAGGATGGGGTTTTATATGTTAAACTCCGGGCGG GTCAGCTCTCCTACAAAGAAGATCCAATGGGATGGCAAAGTTTGTTGGCTCAGACTGTTGCTAACAGGAA CTCTGAAGCCCGGGCTTTCAAGCCAGAAACAATCTCAGCATTCACTTCTGATCCAGCACTTCTGTCATTT GCTGAATATTTCTGCAAGCCAACTGTGAACATGGGTCAGAAACAGGAAATTCTGGATCTCTTTTCTTCAG TACTCTATGAATGTGTTACCCAGGAGACCCCAGAGATGTTGCCTGCATACATAGCAATGGATCAGGCTAT AAGAAGACTTGGGAGAAGAGAAATGTCTGAGACTTCTGAACTTTGGCAGATAAAGTTGGTGTTAGAGTTT TTCAGCTCCCGAAGCCATCAGGAGCGGCTGCAGAACCACCCTAAGCGGGGGCTCTTTATGAACTCGGAAT TCCTCCCTGTTGTGAAGTGCACCATTGATAATACCCTGGACCAGTGGCTACAAGTCGGGGGTGATATGTG TGTGCACGCCTACCTCAGCGGGCAGCCCTTGGAGGAATCACAGCTGAGCATGCTGGCCTGCTTCCTCGTC TACCACTCTGTGCCAGCTCCACAGCACCTGCCACCTATAGGACTAGAAGGGAGCACAAGCTTTGCTGAAC TGCTCTTCAAATTTAAGCAGCTAAAAATGCCAGTGCGAGCTTTGCTGAGATTGGCTCCTTTGCTTCTTGG AAATCCACAGCCAATGGTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216524 representing NM_022662 Red=Cloning site Green=Tags(s) MSNFYEERTTMIAARDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQ KESWQLRKGVSEIGEDVDYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSPVQQALWCDFIISQDKSEK AYSSNEVEKCICILQSSCINMHSIEGKDYIASLPFQVANVWPTKYGLLFERSASSHEVPPGSPREPLPTM FSMLHPLDEITPLVCKSGSLFGSSRVQYVVDHAMKIVFLNTDPSIVMTYDAVQNVHSVWTLRRVKSEEEN VVLKFSEQGGTPQNVATSSSLTAHLRSLSKGDSPVTSPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAAL SRAHSPALGVHSFSGVQRFNISSHNQSPKRHSISHSPNSNSNGSFLAPETEPIVPELCIDHLWTETITNI REKNSQASKVFITSDLCGQKFLCFLVESQLQLRCVKFQESNDKTQLIFGSVTNIPAKDAAPVEKIDTMLV LEGSGNLVLYTGVVRVGKVFIPGLPAPSLTMSNTMPRPSTPLDGVSTPKPLSKLLGSLDEVVLLSPVPEL RDSSKLHDSLYNEDCTFQQLGTYIHSIRDPVHNRVTLELSNGSMVRITIPEIATSELVQTCLQAIKFILP KEIAVQMLVKWYNVHSAPGGPSYHSEWNLFVTCLMNMMGYNTDRLAWTRNFDFEGSLSPVIAPKKARPSE TGSDDDWEYLLNSDYHQNVESHLLNRSLCLSPSEASQMKDEDFSQNLSLDSSTLLFTHIPAIFFVLHLVY EELKLNTLMGEGICSLVELLVQLARDLKLGPYVDHYYRDYPTLVRTTGQVCTIDPGQTGFMHHPSFFTSE PPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYILGDESLVSDESSQYLTRITIAPQKLQVEQ EENRFSFRHSTSVSSLAERLVVWMTNVGFTLRDLETLPFGIALPIRDAIYHCREQPASDWPEAVCLLIGR QDLSKQACEGNLPKGKSVLSSDVPSGTETEEEDDGMNDMNHEVMSLIWSEDLRVQDVRRLLQSAHPVRVN VVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPIPKLNLTGRAPPRNTTVDL NSGNIDVPPNMTSWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLAT LNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHIPALLPPTSTELDVPHNVQVAAVVGIGLV YQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMV GGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVK PEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACL SLGFRFAGSENLSAFNCLHKFAKDFMTYLSAPNASVTGPHNLETCLSVVLLSLAMVMAGSGNLKVLQLCR FLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLY VLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDL SKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSF AEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEF FSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLV YHSVPAPQHLPPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022662
ORF Size	5832 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022662.3, NP_073153.1
RefSeq Size	7753 bp
RefSeq ORF	5835 bp
Locus ID	64682
UniProt ID	Q9H1A4
Domains	PC_rep
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
MW	216.5 kDa
Gene Summary	This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.