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POLR1A (NM_015425) Human Tagged ORF Clone
CAT＃: RC216517
- TrueORF®
POLR1A (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide A, 194kDa (POLR1A)
ORF Plasmid: tGFP
"NM_015425" in other vectors (3)
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CNY 11,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A190; AFDCIN; RPA1; RPA190; RPA194; RPO1-4; RPO14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216517 representing NM_015425
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATCTCCAAGAACATGCCCTGGCGGCGGCTGCAGGGCATTTCCTTCGGGATGTATTCGGCTGAAG AGCTCAAGAAATTAAGTGTTAAATCCATTACGAACCCTCGATACCTGGACAGCCTGGGGAACCCATCGGC AAACGGCCTGTACGATTTAGCTTTGGGCCCTGCAGATTCCAAAGAGGTGTGCTCCACCTGCGTGCAGGAC TTCAGCAACTGTTCTGGGCACCTGGGCCACATTGAGCTCCCACTCACAGTGTATAACCCTCTCCTCTTCG ATAAGCTGTACCTGCTGCTTCGGGGCTCTTGTTTAAACTGCCACATGCTGACTTGTCCCCGGGCCGTGAT TCACCTCTTACTCTGCCAGCTGAGGGTTCTGGAAGTCGGGGCCCTACAAGCAGTCTACGAGCTTGAGAGA ATTCTGAACAGGTTTCTGGAAGAAAATCCCGATCCCTCTGCCTCTGAAATTCGGGAGGAATTAGAACAAT ACACAACTGAAATTGTGCAGAACAACCTCCTGGGGTCCCAGGGCGCACATGTAAAGAACGTGTGTGAGAG CAAGAGCAAGCTCATTGCTCTCTTCTGGAAGGCACATATGAATGCTAAGCGCTGTCCCCACTGCAAGACC GGGCGATCCGTTGTCCGAAAGGAACACAACAGCAAGTTGACTATCACGTTTCCAGCCATGGTGCACAGGA CAGCTGGCCAGAAGGACTCTGAGCCCCTGGGAATTGAGGAAGCTCAGATAGGAAAACGAGGATACTTAAC ACCCACCAGTGCCCGCGAACACCTTTCTGCCCTGTGGAAGAATGAAGGATTCTTTCTGAACTACCTTTTT TCGGGAATGGATGATGATGGTATGGAATCCAGATTCAATCCCAGTGTGTTCTTTCTAGATTTCTTGGTGG TGCCGCCCTCAAGGTATCGCCCAGTCAGTCGCCTAGGAGACCAGATGTTTACTAATGGCCAGACGGTGAA CTTGCAGGCTGTCATGAAGGATGTAGTTCTGATTCGAAAACTTCTGGCATTGATGGCCCAAGAACAGAAG TTGCCAGAGGAAGTGGCCACACCCACTACAGATGAGGAAAAAGACTCTTTGATTGCTATTGACCGATCCT TTTTGAGTACACTTCCAGGCCAGTCCCTCATAGACAAACTTTACAACATTTGGATTCGCCTTCAGAGCCA CGTCAATATTGTGTTTGATAGCGAGATGGACAAACTAATGATGGACAAGTACCCAGGCATTAGGCAGATC CTGGAGAAGAAAGAAGGCCTGTTCCGAAAACACATGATGGGAAAGCGAGTGGACTACGCTGCGCGCTCAG TCATCTGCCCAGACATGTACATCAACACCAACGAAATTGGAATTCCCATGGTGTTTGCCACAAAACTGAC CTACCCACAGCCAGTTACCCCATGGAATGTTCAGGAACTTAGGCAAGCGGTCATCAACGGCCCTAATGTG CACCCAGGAGCCTCCATGGTCATCAATGAGGACGGCAGCCGCACAGCCCTGAGCGCTGTGGACATGACCC AGCGAGAGGCCGTGGCCAAGCAGCTTCTGACCCCAGCCACGGGGGCACCTAAGCCCCAGGGGACAAAAAT TGTGTGCCGGCATGTGAAGAATGGGGACATTCTGCTACTGAACCGACAGCCCACACTGCACAGACCCTCC ATCCAGGCCCACCGTGCCCGCATCCTGCCTGAAGAGAAAGTGCTGCGGCTCCACTATGCCAACTGCAAGG CCTATAATGCCGACTTTGATGGAGACGAGATGAATGCCCATTTCCCCCAGAGTGAGCTGGGCCGGGCCGA GGCCTACGTCCTGGCCTGCACTGATCAGCAGTACCTTGTTCCCAAGGATGGCCAACCATTGGCGGGACTG ATCCAGGATCACATGGTTTCAGGGGCAAGCATGACTACTCGGGGTTGCTTTTTCACCCGGGAGCACTATA TGGAGCTGGTGTACCGAGGACTCACGGACAAAGTGGGGCGCGTGAAGCTCCTTTCTCCTTCCATCCTGAA GCCCTTTCCGCTGTGGACAGGAAAACAGGTTGTGTCAACGCTGCTCATAAATATAATCCCAGAGGACCAC ATCCCACTGAACTTATCTGGAAAGGCGAAAATCACTGGGAAAGCCTGGGTGAAGGAAACTCCTCGATCCG TTCCTGGCTTTAACCCTGACTCGATGTGCGAGTCCCAGGTGATCATCAGGGAAGGGGAGCTGCTCTGCGG AGTGCTGGACAAGGCGCACTATGGGAGCTCCGCCTACGGCCTGGTCCACTGCTGCTATGAGATCTATGGA GGCGAGACCAGCGGCAAGGTTCTAACCTGCCTGGCCCGCCTCTTCACCGCCTACCTGCAGCTCTACAGAG GCTTCACCTTGGGCGTGGAAGACATTTTGGTGAAGCCAAAGGCAGATGTCAAGAGGCAACGTATCATTGA AGAATCCACCCACTGCGGGCCCCAGGCTGTCAGGGCTGCATTAAACCTGCCAGAAGCCGCATCATATGAT GAGGTCCGAGGAAAATGGCAGGATGCCCATCTGGGCAAGGACCAGAGGGATTTTAACATGATTGATCTGA AGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATGCATGCCTTTTGGCCTACACAG ACAGTTCCCAGAGAACAGCCTGCAGATGATGGTGCAGTCGGGAGCCAAAGGTTCAACTGTGAACACGATG CAGATCTCGTGCCTGCTGGGCCAGATTGAACTGGAAGGTCGGAGACCCCCGCTGATGGCGTCTGGCAAGT CACTGCCCTGCTTTGAGCCTTATGAGTTCACCCCCAGGGCTGGTGGCTTTGTCACTGGCAGGTTCCTCAC CGGCATCAAACCTCCTGAGTTCTTCTTCCACTGCATGGCAGGACGAGAGGGCCTGGTGGACACTGCTGTG AAAACCAGCCGCTCAGGCTATCTCCAAAGGTGCATCATCAAGCACCTAGAGGGGCTGGTCGTGCAGTATG ATCTCACGGTCCGTGACAGTGACGGCAGTGTGGTGCAGTTCCTGTATGGGGAGGATGGCCTGGACATCCC CAAGACACAGTTCCTGCAGCCCAAGCAGTTCCCCTTCCTGGCCAGCAACTACGAGGTGATAATGAAATCA CAGCATCTCCATGAAGTTTTATCCAGAGCAGATCCCAAAAAAGCTCTCCACCACTTCAGAGCTATCAAAA AATGGCAAAGCAAGCACCCCAACACCCTGCTGAGAAGAGGCGCCTTCTTGAGTTATTCCCAGAAAATTCA GGAAGCTGTGAAAGCCCTGAAACTTGAGAGTGAAAACCGCAATGGCCGCAGCCCTGGGACTCAGGAGATG CTGAGGATGTGGTATGAGTTGGATGAGGAAAGCCGAAGGAAATACCAGAAGAAGGCGGCCGCTTGTCCTG ACCCCAGTCTGTCTGTCTGGCGTCCTGACATCTACTTTGCATCAGTGTCAGAAACATTTGAAACAAAGGT TGATGACTACAGTCAAGAGTGGGCAGCTCAAACAGAGAAGAGTTATGAGAAATCAGAGCTTTCTCTCGAC AGGTTGAGGACCTTGCTGCAGCTGAAGTGGCAGCGCTCACTGTGTGAGCCGGGCGAGGCTGTGGGCCTGC TGGCTGCCCAGAGCATCGGAGAGCCCTCCACCCAGATGACCCTCAACACCTTCCACTTTGCAGGCAGAGG CGAGATGAACGTCACCCTGGGCATTCCAAGGTTGCGGGAGATTCTCATGGTGGCCAGCGCCAACATCAAG ACACCCATGATGAGCGTGCCCGTGCTCAACACCAAGAAAGCCCTGAAGAGAGTGAAAAGCCTGAAGAAGC AACTCACCAGGGTGTGCTTGGGGGAGGTGTTGCAGAAAATTGACGTCCAGGAGTCCTTCTGTATGGAAGA AAAACAGAACAAATTCCAGGTGTACCAGCTGCGGTTTCAGTTCCTGCCACATGCATATTACCAGCAGGAG AAGTGCCTGAGACCCGAGGACATCCTGCGCTTCATGGAAACAAGATTCTTTAAACTTCTGATGGAATCCA TCAAAAAGAAGAATAATAAAGCATCAGCTTTCAGGAACGTAAACACTCGAAGAGCTACACAGCGGGATCT GGACAACGCTGGGGAGTTGGGGAGGAGTCGGGGAGAGCAGGAGGGTGATGAGGAAGAGGAGGGGCACATT GTGGATGCTGAAGCTGAGGAGGGGGACGCCGATGCCTCTGATGCCAAACGCAAGGAGAAGCAGGAGGAGG AGGTTGATTATGAGAGTGAGGAAGAGGAGGAGAGGGAGGGCGAGGAGAACGACGATGAAGACATGCAGGA GGAACGAAATCCCCACAGGGAAGGTGCTCGAAAGACCCAAGAGCAAGATGAAGAGGTGGGCTTAGGCACT GAGGAGGACCCGTCCCTTCCCGCCCTCCTGACGCAGCCCCGGAAACCCACCCACAGCCAGGAGCCCCAGG GGCCCGAGGCCATGGAGCGCCGGGTCCAGGCTGTGCGTGAGATCCACCCGTTCATAGATGACTACCAGTA CGACACCGAGGAGAGCCTGTGGTGCCAGGTGACAGTGAAGCTCCCTCTGATGAAGATCAACTTTGACATG AGCTCCCTGGTAGTATCTTTGGCCCATGGTGCCGTCATCTATGCGACCAAGGGCATCACTCGGTGCCTCC TGAATGAAACAACCAACAATAAGAACGAGAAGGAGCTTGTGCTAAACACAGAAGGAATCAACCTCCCAGA GCTATTCAAGTATGCAGAGGTCCTGGATCTGCGCCGCCTCTACTCCAACGACATCCACGCCATAGCCAAC ACGTATGGCATTGAGGCCGCGCTGCGGGTGATCGAGAAGGAGATCAAGGATGTGTTTGCCGTGTATGGCA TCGCGGTCGACCCTCGCCATCTCTCCCTGGTTGCTGATTATATGTGCTTCGAGGGTGTTTACAAGCCACT GAATCGCTTTGGGATCCGGTCAAACTCTTCCCCGCTACAGCAGATGACATTTGAAACCAGCTTCCAGTTT CTGAAGCAAGCCACCATGCTGGGATCCCACGATGAGCTGAGGTCTCCTTCTGCCTGCCTTGTGGTCGGGA AGGTCGTCAGGGGCGGGACAGGCCTGTTCGAGCTCAAGCAGCCTCTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216517 representing NM_015425
Red=Cloning site Green=Tags(s) MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGPADSKEVCSTCVQD FSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELER ILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKT GRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLF SGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQK LPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQI LEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNV HPGASMVINEDGSRTALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPS IQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGL IQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDH IPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYG GETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYD EVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTM QISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAV KTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKS QHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEM LRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLD RLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIK TPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQE KCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHI VDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGT EEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDM SSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIAN TYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQF LKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015425 |
| ORF Size | 5160 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015425.6 |
| RefSeq Size | 6637 bp |
| RefSeq ORF | 5163 bp |
| Locus ID | 25885 |
| UniProt ID | O95602 |
| Domains | RNA_pol_Rpb1_2, RPOLA_N, RNA_pol_Rpb1_3, RNA_pol_Rpb1_1, RNA_pol_Rpb1_5, RNA_pol_Rpb1_4 |
| Protein Families | Transcription Factors |
| Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 195.3 kDa |
| Gene Summary | The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Scleroderma patients with antibodies against the large subunits of both RNA polymerases-I and -III are protected against cancer
,Shah, AA;Laiho, M;Rosen, A;Casciola-Rosen, L;,
Arthritis & rheumatology (Hoboken, N.J.) 2019
,PubMed ID 30888702
[POLR1A]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RG216517 | POLR1A (tGFP-tagged) - Human polymerase (RNA) I polypeptide A, 194kDa (POLR1A) |
CNY 13,160.00 |
|
| SC318659 | POLR1A (untagged)-Human polymerase (RNA) I polypeptide A, 194kDa (POLR1A) |
CNY 26,790.00 |
|
| SC318660 | POLR1A (untagged)-Human polymerase (RNA) I polypeptide A, 194kDa (POLR1A) |
CNY 26,790.00 |
