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Semaphorin 3E (SEMA3E) (NM_012431) Human Tagged ORF Clone
CAT＃: RC216038
SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1
ORF Plasmid: tGFP
"NM_012431" in other vectors (6)
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CNY 8,512.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | coll-5; M-SEMAH; M-SemaK; SEMAH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216038 representing NM_012431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCGCGGGGCACATTATCACCTTGCTCCTGTGGGGTTACTTACTGGAGCTTTGGACAGGAGGTC ATACAGCTGATACTACCCACCCCCGGTTACGCCTGTCACATAAAGAGCTCTTGAATCTGAACAGAACATC AATATTTCATAGCCCTTTTGGATTTCTTGATCTCCATACAATGCTGCTGGATGAATATCAAGAGAGGCTC TTCGTGGGAGGCAGGGACCTTGTATATTCCCTCAGCTTGGAGAGAATCAGTGACGGCTATAAAGAGATAC ACTGGCCGAGTACAGCTCTAAAAATGGAAGAATGCATAATGAAGGGAAAAGATGCGGGTGAATGTGCAAA TTATGTTCGGGTTTTGCATCACTATAACAGGACACACCTTCTGACCTGTGGTACTGGAGCTTTTGATCCA GTTTGTGCCTTCATCAGAGTTGGATATCATTTGGAGGATCCTCTGTTTCACCTGGAATCACCCAGATCTG AGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAGCTCCTCCTTCATCTCCACTTTAATTGGTAGTGAATT GTTTGCTGGACTCTACAGTGACTACTGGAGCAGAGACGCTGCGATCTTCCGCAGCATGGGGCGACTGGCC CATATCCGCACTGAGCATGACGATGAGCGTCTGTTGAAAGAACCAAAATTTGTAGGTTCATACATGATTC CTGACAATGAAGACAGAGATGACAACAAAGTATATTTCTTTTTTACTGAGAAGGCACTGGAGGCAGAAAA CAATGCTCACGCAATTTACACCAGGGTCGGGCGACTCTGTGTGAATGATGTAGGAGGGCAGAGAATACTG GTGAATAAGTGGAGCACTTTCCTAAAAGCGAGACTCGTTTGCTCAGTACCAGGAATGAATGGAATTGACA CATATTTTGATGAATTAGAGGACGTTTTTTTGCTACCTACCAGAGATCATAAGAATCCAGTGATATTTGG ACTCTTTAACACTACCAGTAATATTTTTCGAGGGCATGCTATATGTGTCTATCACATGTCTAGCATTCGG GCAGCCTTCAACGGACCATATGCACATAAGGAAGGACCTGAATACCACTGGTCAGTCTATGAAGGAAAAG TCCCTTATCCAAGGCCTGGTTCTTGTGCCAGCAAAGTAAATGGAGGGAGATACGGAACCACCAAGGACTA TCCTGATGATGCCATCCGATTTGCAAGAAGTCATCCACTAATGTACCAGGCCATAAAACCTGCCCATAAA AAACCAATATTGGTAAAAACAGATGGAAAATATAACCTGAAACAAATAGCAGTAGATCGAGTGGAAGCTG AGGATGGCCAATATGACGTCTTGTTTATTGGGACAGATAATGGAATTGTGCTGAAAGTAATCACAATTTA CAACCAAGAAATGGAATCAATGGAAGAAGTAATTCTAGAAGAACTTCAGATATTCAAGGATCCAGTTCCT ATTATTTCTATGGAGATTTCTTCAAAGCGGCAACAGCTGTATATTGGATCTGCTTCTGCTGTGGCTCAAG TCAGATTCCATCACTGTGACATGTATGGAAGTGCTTGTGCTGACTGCTGCCTGGCTCGAGACCCTTACTG TGCCTGGGATGGCATATCCTGCTCCCGGTATTACCCAACAGGCACACATGCAAAAAGGCGTTTCCGGAGA CAAGATGTTCGACATGGAAATGCAGCTCAGCAGTGCTTTGGACAACAGTTTGTTGGGGATGCTTTGGATA AGACTGAAGAACATCTGGCTTATGGCATAGAGAACAACAGTACTTTGCTGGAATGTACCCCACGATCTTT ACAAGCGAAAGTTATCTGGTTTGTACAGAAAGGACGTGAGACAAGAAAAGAGGAGGTGAAGACAGATGAC AGAGTGGTTAAGATGGACCTTGGTTTACTCTTCCTAAGGTTACACAAATCAGATGCTGGGACCTATTTTT GCCAGACAGTAGAGCATAGCTTTGTCCATACGGTCCGTAAAATCACCTTGGAGGTAGTGGAAGAGGAGAA AGTCGAGGATATGTTTAACAAGGACGATGAGGAGGACAGGCATCACAGGATGCCTTGTCCTGCTCAGAGT AGCATCTCGCAGGGAGCAAAACCATGGTACAAGGAATTCTTGCAGCTGATCGGTTATAGCAACTTCCAGA GAGTGGAAGAATACTGCGAGAAAGTATGGTGCACAGATAGAAAGAGGAAAAAGCTTAAAATGTCACCCTC CAAGTGGAAGTATGCCAACCCTCAGGAAAAGAAGCTCCGTTCCAAACCTGAGCATTACCGCCTGCCCAGG CACACGCTGGACTCC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RC216038 representing NM_012431
Red=Cloning site Green=Tags(s) MASAGHIITLLLWGYLLELWTGGHTADTTHPRLRLSHKELLNLNRTSIFHSPFGFLDLHTMLLDEYQERL FVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDP VCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLA HIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGGQRIL VNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHAICVYHMSSIR AAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPLMYQAIKPAHK KPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVP IISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRR QDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKEEVKTDD RVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQS SISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYRLPR HTLDS TRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012431 |
| ORF Size | 2325 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012431.3 |
| RefSeq Size | 6474 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 9723 |
| UniProt ID | O15041 |
| Domains | Sema, ig, PSI |
| Protein Families | Secreted Protein |
| Protein Pathways | Axon guidance |
| MW | 89 kDa |
| Gene Summary | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance
,van der Klaauw, AA;Croizier, S;Mendes de Oliveira, E;Stadler, LKJ;Park, S;Kong, Y;Banton, MC;Tandon, P;Hendricks, AE;Keogh, JM;Riley, SE;Papadia, S;Henning, E;Bounds, R;Bochukova, EG;Mistry, V;O'Rahilly, S;Simerly, RB;, ;, ;Minchin, JEN;Barroso, I;Jones, EY;Bouret, SG;Farooqi, IS;,
Cell
,PubMed ID 30661757
[Semaphorin 3E]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216038L1 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1, Myc-DDK-tagged |
CNY 10,912.00 |
|
| RC216038L2 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1, mGFP tagged |
CNY 10,912.00 |
|
| RC216038L3 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216038L4 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216038 | SEMA3E (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1 |
CNY 10,112.00 |
|
| SC115386 | SEMA3E (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1 |
CNY 8,528.00 |
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