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RAD17 (NM_133342) Human Tagged ORF Clone
CAT＃: RC215866
RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 5
ORF Plasmid: tGFP
"NM_133342" in other vectors (4)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215866 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTAC TATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAA AGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAATT CAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGC TGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAA CAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTAT CAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGATGATTT CAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTTTTCAAA GAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGA AGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAG GAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATAAT CAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGG CACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAAT TACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGTGCAATA AACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAAGGAATGTCTTTAA AATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTTGAAAATCAAGAGGT CCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAA AGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACAT TACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCA CCAAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCA GATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACATCTATAGCTACGAGAG GTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTT GCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTT CCTGACTTCTGCCTACCAGCTTTATGCCGCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTC CAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGG AAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCC CAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCT GGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCA AGGAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215866 protein sequence
Red=Cloning site Green=Tags(s) X*IR*QTGLTHHLMIF*SVVASLLLLPHH*V*ITQVIEEKMGLLH*KAADFQREKEEIYLP*NRFMV*KI QKNICLKMNHGWININQKLSMNLLCIKRKLKKSKPG*KLKF*KGNQNRVDLFY**QVLLDVERQRP*KYY QRSMVFKYKSGLIQFYQTSKKMISRGCLILNQASICFPISLR*QFSKSFY*ERQSITSYKCLEMI*ELIR R*FWLKIYLTSFIGILILYMKF*GSM*GLVDVLLYL*SRTVSVEIIIKGYCFPKKFRKSVLSQILVSTLW HQQL**NFLIE**L*KLTRMEEKLLSLTKLL*SCSVRDVLVISEVQ*TASSFLLQKEKTTYGQGKKECL* NQMLCCQNQNEEKNLIGFLKIKRSKLLVAKMFLCFSSELWGKFYIVKEHL*QN*THLGCPLIYQNMNGIH YLLNLRR**KCHTCLETYLIYIFTKTT*ISSWKLMIL*EPVNF*VLQISSVVTGIHALYSGNIAHL*LRE V*CIPTKPEDMLIAKEEDQVFDPCTNLSGF**IKSIGKIAWQQKHFFLTSAYQLYAAKLSYCHTLLY*PF Q*EIKLRFLLSKILEGSL*SDTLED*KWKP*LTGNME**TLTAEMKPSLMEDILQRNLWVNPLKPLCRKP GLFL*VRIVPVNCLLASPSPFQPKETWKKT***KTTRVMG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133342 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133342.3 |
| RefSeq Size | 3054 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Domains | Rad17 |
| Protein Families | Druggable Genome |
| MW | 75.9 kDa |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TOP1 inhibition induces bifurcated JNK/MYC signaling that dictates cancer cell sensitivity
,null,
International Journal of Biological Sciences
,PubMed ID 35844787
[RAD17]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215866L3 | Lenti-ORF clone of RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 5 |
CNY 5,890.00 |
|
| RC215866L4 | Lenti-ORF clone of RAD17 (mGFP-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 5 |
CNY 5,890.00 |
|
| RG215866 | RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 5 |
CNY 4,370.00 |
|
| SC109698 | RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 5 |
CNY 7,220.00 |
