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IRAG1 (NM_001100167) Human Tagged ORF Clone
CAT＃: RC215802
- TrueORF®
MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 4
ORF Plasmid: tGFP
"NM_001100167" in other vectors (4)
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CNY 5,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IRAG; JAW1L; MRVI1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215802 representing NM_001100167
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGAACAGCCCTCAGCCTGGCCCCGTGGAGAGCGAGCTGGGGAAGCAGCTCTTGAAAACGGGCT GGGAGGGCAGCCCTCTGCCGAGAAGTCCAACCCAGGATGCGGCAGGAGTGGGTCCCCCAGCCTCCCAGGG GAGAGGCCCAGCTGGAGAGCCGATGGGGCCCGAGGCTGGCTCCAAAGCTGAGCTTCCACCCACTGTGTCC CGGCCCCCGCTGCTGCGAGGGCTCTCCTGGGACAGTGGCCCTGAAGAACCTGGCCCCCGGCTGCAGAAAG TGCTTGCCAAGCTGCCACTGGCAGAGGAAGAAAAGCGTTTTGCAGGCAAGGCCGGCGGCAAGCTGGCCAA GGCCCCTGGTCTCAAAGACTTTCAGATACAAGTGCAGCCCGTGCGGATGCAGAAACTGACCAAGCTCCGA GAGGAGCACATCCTGATGAGAAATCAGAACTTAGTGGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTG AGCAGGAAAAAGGGCTTCCTTCTGAACTCTCCCCAGCTATTGAGGAAGAAGAGTCAAAGAGTGGCTTAGA TGTCATGCCTAATATTTCTGATGTGCTGCTGCGCAAACTGCGGGTCCACAGGAGTCTCCCTGGAAGTGCC CCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTTTGTGCAACTGTCCTTGGCCTTTAGAAATGACAGCT ACACTCTGGAATCTAGAATTAACCAGGCTGAAAGGGAACGCAACCTGACAGAGGAGAACACTGAGAAAGA ACTGGAAAACTTCAAAGCTTCCATTACGTCCTCAGCTTCACTCTGGCACCACTGTGAGCACCGGGAAACC TACCAGAAGTTGCTGGAGGACATCGCTGTCCTGCACCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGG TGGTAGGCGCCGTCCGCCAGGAAAAGCGCATGTCGAAAGCAACGGAAGTGATGATGCAGTATGTGGAGAA TCTAAAGAGGACGTATGAGAAGGACCATGCGGAGCTCATGGAGTTTAAAAAGCTTGCAAATCAGAATTCA AGCCGCAGCTGTGGCCCCTCTGAAGATGGGGTCCCTCGCACGGCACGGTCCATGTCCCTCACGCTGGGAA AGAATATGCCTCGCCGGAGGGTCAGCGTTGCTGTGGTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCA AACTCCCAGCTCATCATCCATTCCCTCCTTACCAGCCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTA CCTGTCACTTCAGCACTGCCTGCACTTTTGGAAAATGGAAAGACAAATGGGGACCCAGATTGTGAAGCCT CTGCTCCTGCGCTGACCCTGAGCTGCCTGGAGGAGCTTAGTCAGGAGACCAAGGCCAGGATGGAGGAAGA AGCCTACAGCAAGGGATTCCAAGAAGGTCTAAAGAAGACCAAAGAACTTCAAGACCTGAAGGAGGAGGAG GAAGAACAGAAGAGTGAGAGTCCTGAGGAACCTGAAGAGGTAGAAGAAACTGAGGAAGAGGAAAAGGGCC CAAGAAGCAGCAAACTTGAAGAATTGGTCCATTTCTTACAAGTCATGTATCCCAAACTGTGTCAGCACTG GCAAGTGATCTGGATGATGGCTGCAGTGATGCTGGTCTTGACTGTTGTGCTGGGGCTCTACAATTCCTAT AACTCTTGTGCAGAGCAGGCTGATGGGCCCCTTGGAAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCT GGTGGAGCTCAGGACTCCAGCATGAGCAGCCTACAGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215802 representing NM_001100167
Red=Cloning site Green=Tags(s) MALNSPQPGPVESELGKQLLKTGWEGSPLPRSPTQDAAGVGPPASQGRGPAGEPMGPEAGSKAELPPTVS RPPLLRGLSWDSGPEEPGPRLQKVLAKLPLAEEEKRFAGKAGGKLAKAPGLKDFQIQVQPVRMQKLTKLR EEHILMRNQNLVGLKLPDLSEAAEQEKGLPSELSPAIEEEESKSGLDVMPNISDVLLRKLRVHRSLPGSA PPLTEKEVENVFVQLSLAFRNDSYTLESRINQAERERNLTEENTEKELENFKASITSSASLWHHCEHRET YQKLLEDIAVLHRLAARLSSRAEVVGAVRQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNS SRSCGPSEDGVPRTARSMSLTLGKNMPRRRVSVAVVPKFNALNLPGQTPSSSSIPSLPALSESPNGKGSL PVTSALPALLENGKTNGDPDCEASAPALTLSCLEELSQETKARMEEEAYSKGFQEGLKKTKELQDLKEEE EEQKSESPEEPEEVEETEEEEKGPRSSKLEELVHFLQVMYPKLCQHWQVIWMMAAVMLVLTVVLGLYNSY NSCAEQADGPLGRSTCSAAQRDSWWSSGLQHEQPTEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100167 |
| ORF Size | 1791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100167.3 |
| RefSeq Size | 6040 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 10335 |
| UniProt ID | Q9Y6F6 |
| Protein Families | Transmembrane |
| Protein Pathways | Vascular smooth muscle contraction |
| MW | 65.9 kDa |
| Gene Summary | This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215802L3 | Lenti-ORF clone of MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 4 |
CNY 7,890.00 |
|
| RC215802L4 | Lenti-ORF clone of MRVI1 (mGFP-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 4 |
CNY 7,890.00 |
|
| RG215802 | MRVI1 (tGFP-tagged) - Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 4 |
CNY 6,560.00 |
|
| SC316790 | MRVI1 (untagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 4 |
CNY 7,220.00 |
