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KCNC1 (NM_004976) Human Tagged ORF Clone
CAT＃: RC215716
KCNC1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B
ORF Plasmid: tGFP
"NM_004976" in other vectors (6)
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CNY 5,704.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EPM7; KV3.1; KV4; NGK2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215716 representing NM_004976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAAGGGGACGAGAGCGAGCGCATCGTGATCAACGTGGGCGGCACGCGCCACCAGACGTACCGCT CGACCCTGCGCACGCTGCCCGGCACGCGGCTCGCCTGGCTGGCGGAGCCCGACGCCCACAGCCACTTCGA CTATGACCCGCGTGCTGACGAGTTCTTCTTCGACCGCCACCCCGGCGTCTTCGCGCACATCCTGAACTAC TACCGCACGGGCAAGCTGCACTGCCCAGCCGACGTGTGCGGGCCGCTCTACGAGGAGGAGCTGGCCTTCT GGGGCATCGACGAGACCGACGTGGAGCCCTGCTGCTGGATGACGTACCGCCAGCACCGCGACGCCGAGGA GGCTCTGGACAGCTTCGGCGGCGCTCCTCTGGACAACAGCGCCGACGACGCGGACGCCGACGGCCCTGGC GACTCGGGCGACGGCGAGGACGAGCTGGAGATGACCAAGCGCCTGGCGCTCAGTGACTCCCCGGATGGCC GGCCTGGCGGCTTTTGGCGCCGCTGGCAGCCGCGCATCTGGGCGCTCTTCGAGGACCCGTACTCGTCCCG CTACGCGCGGTATGTGGCCTTCGCTTCCCTCTTCTTCATCCTGGTCTCCATCACCACCTTCTGCCTGGAG ACCCACGAGCGCTTCAACCCCATCGTGAACAAGACGGAGATCGAGAACGTTCGCAATGGCACGCAAGTGC GCTACTACCGGGAGGCCGAGACGGAGGCCTTCCTTACCTACATCGAGGGCGTCTGTGTGGTCTGGTTCAC CTTCGAGTTCCTCATGCGTGTCATCTTCTGCCCCAACAAGGTAGAGTTCATCAAGAACTCGCTCAACATC ATTGACTTTGTGGCCATCCTGCCCTTCTACCTGGAGGTGGGGCTGAGCGGCCTGTCCTCCAAGGCAGCCA AGGACGTGCTGGGCTTCCTGCGCGTCGTCCGCTTCGTGCGCATCTTGCGCATCTTTAAGCTGACCCGCCA CTTTGTGGGCCTGCGGGTCCTGGGCCACACGCTCCGAGCCAGCACCAACGAGTTCCTGCTGCTCATCATC TTCCTGGCCTTGGGCGTGCTGATCTTCGCCACCATGATCTACTACGCCGAGAGGATAGGGGCACAGCCCA ATGACCCCAGCGCCAGTGAGCACACGCACTTTAAGAACATCCCCATCGGCTTCTGGTGGGCCGTGGTCAC CATGACGACCCTGGGCTATGGAGACATGTACCCGCAGACGTGGTCCGGCATGCTGGTGGGGGCTCTGTGT GCGCTGGCGGGCGTGCTCACCATCGCCATGCCCGTGCCCGTCATCGTGAACAATTTCGGGATGTATTACT CCTTAGCCATGGCTAAGCAGAAACTACCAAAGAAAAAAAAGAAGCATATTCCGCGGCCACCGCAGCTGGG ATCTCCCAATTATTGTAAATCTGTCGTAAACTCTCCACACCACAGTACTCAGAGTGACACATGTCCGCTG GCCCAGGAAGAAATTTTAGAAATTAACAGAGCAGGTAGGAAACCTCTTAGAGGCATGTCGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215716 representing NM_004976
Red=Cloning site Green=Tags(s) MGQGDESERIVINVGGTRHQTYRSTLRTLPGTRLAWLAEPDAHSHFDYDPRADEFFFDRHPGVFAHILNY YRTGKLHCPADVCGPLYEEELAFWGIDETDVEPCCWMTYRQHRDAEEALDSFGGAPLDNSADDADADGPG DSGDGEDELEMTKRLALSDSPDGRPGGFWRRWQPRIWALFEDPYSSRYARYVAFASLFFILVSITTFCLE THERFNPIVNKTEIENVRNGTQVRYYREAETEAFLTYIEGVCVVWFTFEFLMRVIFCPNKVEFIKNSLNI IDFVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLII FLALGVLIFATMIYYAERIGAQPNDPSASEHTHFKNIPIGFWWAVVTMTTLGYGDMYPQTWSGMLVGALC ALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKKKHIPRPPQLGSPNYCKSVVNSPHHSTQSDTCPL AQEEILEINRAGRKPLRGMSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004976 |
| ORF Size | 1533 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004976.4 |
| RefSeq Size | 1602 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 3746 |
| UniProt ID | P48547 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 57.8 kDa |
| Gene Summary | This gene encodes a member of a family of integral membrane proteins that mediate the voltage-dependent potassium ion permeability of excitable membranes. Alternative splicing is thought to result in two transcript variants encoding isoforms that differ at their C-termini. These isoforms have had conflicting names in the literature: the longer isoform has been called both "b" and "alpha", while the shorter isoform has been called both "a" and "beta" (PMIDs 1432046, 12091563). [provided by RefSeq, Oct 2014] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Utilising Automated Electrophysiological Platforms in Epilepsy Research
,Milligan, CJ;Pachernegg, S;,
Methods Mol Biol
,PubMed ID 33119850
[KCNC1]
|
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Encephalopathies with KCNC1 variants: genotype-phenotype-functional correlations
,Cameron, JM;Maljevic, S;Nair, U;Aung, YH;Cogné, B;Bézieau, S;Blair, E;Isidor, B;Zweier, C;Reis, A;Koenig, MK;Maarup, T;Sarco, D;Afenjar, A;Huq, AHMM;Kukolich, M;Billette de Villemeur, T;Nava, C;Héron, B;Petrou, S;Berkovic, SF;,
Ann Clin Transl Neurol
,PubMed ID 31353855
[KCNC1]
|
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KCNC1-related disorders: KCNC1-related disorders: new de novo variants expand the phenotypic spectrum
,Park, J;Koko, M;Hedrich, U;Hermann, A;Cremer, K;Haberlandt, E;Grimmel, M;Alhaddad, B;Beck‐Woedl, S;Harrer, M;Karall, D;Kingelhoefer, L;Tzschach, A;Matthies, L;Strom, T;Ringelstein, E;Sturm, M;Engels, H;Wolff, M;Lerche, H;Haack, T;,
Ann Clin Transl Neurol
,PubMed ID 31353862
[KCNC1]
|
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Myoclonus epilepsy and ataxia due to KCNC1 mutation: Analysis of 20 cases and K(+) channel properties
,Oliver, KL;Franceschetti, S;Milligan, CJ;Muona, M;Mandelstam, SA;Canafoglia, L;Boguszewska-Chachulska, AM;Korczyn, A;Bisulli, F;Di Bonaventura, C;Ragona, F;Michelucci, R;Ben-Zeev, B;Straussberg, R;Panzica, F;Massano, J;Friedman, D;Crespel, A;Engelsen, BA;Andermann, F;Andermann, E;Spodar, K;Lasek-Bal, A;Riguzzi, P;Pasini, E;Tinuper, P;Licchetta, L;Gardella, E;Lindenau, M;Wulf, A;Møller, RS;Benninger, F;Afawi, Z;Rubboli, G;Reid, CA;Maljevic, S;Lerche, H;Lehesjoki, AE;Petrou, S;Berkovic, SF;,
Ann. Neurol.
,PubMed ID 28380698
[KCNC1]
|
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A recurrent de novo mutation in KCNC1 causes progressive myoclonus epilepsy
,Muona, M;Berkovic, SF;Dibbens, LM;Oliver, KL;Maljevic, S;Bayly, MA;Joensuu, T;Canafoglia, L;Franceschetti, S;Michelucci, R;Markkinen, S;Heron, SE;Hildebrand, MS;Andermann, E;Andermann, F;Gambardella, A;Tinuper, P;Licchetta, L;Scheffer, IE;Criscuolo, C;Filla, A;Ferlazzo, E;Ahmad, J;Ahmad, A;Baykan, B;Said, E;Topcu, M;Riguzzi, P;King, MD;Ozkara, C;Andrade, DM;Engelsen, BA;Crespel, A;Lindenau, M;Lohmann, E;Saletti, V;Massano, J;Privitera, M;Espay, AJ;Kauffmann, B;Duchowny, M;Møller, RS;Straussberg, R;Afawi, Z;Ben-Zeev, B;Samocha, KE;Daly, MJ;Petrou, S;Lerche, H;Palotie, A;Lehesjoki, AE;,
Nat. Genet.
,PubMed ID 25401298
[KCNC1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215716L1 | Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, Myc-DDK-tagged |
CNY 8,104.00 |
|
| RC215716L2 | Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, mGFP tagged |
CNY 6,370.00 |
|
| RC215716L3 | Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC215716L4 | Lenti ORF clone of Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B, mGFP tagged |
CNY 6,370.00 |
|
| RG215716 | KCNC1 (tGFP-tagged) - Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B |
CNY 7,304.00 |
|
| SC310304 | KCNC1 (untagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B |
CNY 5,720.00 |
