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SRGAP2 (NM_001042758) Human Tagged ORF Clone
CAT＃: RC215563
- TrueORF®
SRGAP2 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2
ORF Plasmid: tGFP
"NM_001042758" in other vectors (4)
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CNY 8,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGAP34; FNBP2; SRGAP2A; SRGAP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215563 representing NM_001042758
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGCAGAGTACGATACTCAGGTCAAAGAGA TCCGTGCTCAGCTCACAGAGCAGATGAAATGCCTGGACCAGCAGTGTGAGCTTCGGGTGCAACTGTTGCA GGACCTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAGATGGACTACTCCCGCAACCTGGAGAAGCTG GCAGAACGCTTCCTGGCCAAGACACGCAGCACCAAGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCT CTCCAGTCAACTGCTGGAATCTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAG TGACATCTACCTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA AAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTCTACTCGGTGA TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAAAGGAGGCGGAGAAGCA GGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGACCGGCAGACCCCACGCTCCCCTGACTCCACG GCCAACGTTCGCATTGAGGAGAAACATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA AGCGTCAAGCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTACTTGCTGGCTTT GGAGGCAACCAATGCATCTGTCTTCAAGTACTACATCCATGACCTATCTGACCTTATTGATCAGTGTTGT GACTTAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTAAACCTGGAAC AGTCGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACCTGGATGCCACCAGTGACAAGCA GCGCCTCATGGAGATGTACAACAACGTCTTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCACATGGGG GATATGGCTTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTACAGAGATGCCAACAACTGC AGTCTCGCTTATCCACTCTAAAGATTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAAC CATCCAGGACATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGGAG TCCGTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCCAACCAGC AAGAGACAGAGCAGTTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGCAGGAACCTCATCACCAAGTT ACAAGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGATTGCAGTCTAGCCAGG CGCAGCTCAACTGTGAGGAAACAGGACTCCAGCCAGGCAATTCCTCTGGTGGTGGAAAGCTGTATCCGGT TTATCAGCAGACACGGACTACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAATGA CATCAAAAATGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAACGACCATGACATGGATTCC ATAGCTGGTGTCCTGAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCTTTCATG ACCTGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCACATCCGGAAAGTCCTCCTAGT CCTGCCCAAAACCACTCTGATTATCATGAGATACCTCTTTGCCTTCCTCAATCATTTATCACAGTTCAGT GAAGAGAACATGATGGACCCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGAGG GCCACGACCAGGTGTCCTGCCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAGCATGAGAA CATCTTCCCAAGCCCCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT GATAGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAGAGCACCACACGA GCGATGACGAATGTGAGCCCATCGAGGCCATTGCCAAGTTTGACTACGTGGGCCGGACAGCCCGAGAGCT GTCCTTTAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGGGCTTCCGACGACTGGTGGGAAGGCCGGCAC AATGGCATCGACGGACTCATCCCCCATCAGTACATCGTGGTCCAAGACACCGAGGACGGTGTCGTGGAGA GGTCCAGCCCCAAGTCTGAGATTGAGGTCATTTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAGCGGG GGCCAGCTGTCCCAGTGGGGGTCATGTAGCCGATATTTATCTTGCAAACATCAACAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC215563 representing NM_001042758
Red=Cloning site Green=Tags(s) MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCC DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG DMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR RSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS IAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFS EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYC DSPHGETTSVEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINK SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042758 |
| ORF Size | 2508 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042758.1, NP_001036223.1 |
| RefSeq Size | 3714 bp |
| RefSeq ORF | 2510 bp |
| Locus ID | 23380 |
| Protein Pathways | Axon guidance |
| MW | 95.7 kDa |
| Gene Summary | This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215563L3 | Lenti-ORF clone of SRGAP2 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2 |
CNY 10,260.00 |
|
| RC215563L4 | Lenti-ORF clone of SRGAP2 (mGFP-tagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2 |
CNY 10,260.00 |
|
| RG215563 | SRGAP2 (tGFP-tagged) - Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2 |
CNY 9,220.00 |
|
| SC311492 | SRGAP2 (untagged)-Human SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2), transcript variant 2 |
CNY 16,250.00 |
