United States
Germany
Japan
United Kingdom
China
ALG12 (NM_024105) Human Tagged ORF Clone
CAT＃: RC215501
- TrueORF®
ALG12 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12)
ORF Plasmid: tGFP
"NM_024105" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDG1G; ECM39; hALG12; PP14673 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCTGCTGCTGGGGCTGCTGGTGGCCGTAGCCACTGTCC ACCTGGTCATCTGTCCCTACACCAAAGTGGAGGAGAGCTTCAACCTGCAGGCCACACATGACCTGCTCTA CCACTGGCAAGACCTGGAGCAGTACGACCATCTTGAGTTCCCCGGAGTCGTCCCCAGGACGTTCCTCGGG CCAGTGGTGATCGCAGTGTTCTCCAGCCCCGCGGTTTACGTGCTTTCGCTGTTAGAAATGTCCAAGTTTT ACTCTCAGCTAATAGTTAGAGGAGTGCTTGGACTCGGCGTGATTTTTGGACTCTGGACGTTACAAAAGGA AGTGAGACGGCACTTCGGGGCCATGGTGGCCACCATGTTCTGCTGGGTGACGGCCATGCAGTTCCACCTG ATGTTCTACTGCACGCGGACACTGCCCAATGTGCTGGCCCTGCCTGTAGTCCTGCTGGCCCTCGCGGCCT GGCTGCGGCACGAGTGGGCCCGCTTCATCTGGCTGTCAGCCTTCGCCATCATCGTGTTCAGGGTGGAGCT GTGCCTGTTCCTGGGCCTCCTGCTGCTGCTGGCCTTGGGCAACCGAAAGGTTTCTGTAGTCAGAGCCCTT CGCCACGCCGTCCCGGCAGGGATCCTCTGTTTAGGACTGACGGTTGCTGTGGACTCTTATTTTTGGCGGC AGCTCACTTGGCCGGAAGGAAAGGTGCTTTGGTACAACACTGTCCTGAACAAAAGCTCCAACTGGGGGAC CTCCCCGCTGCTGTGGTACTTCTACTCAGCCCTGCCCCGCGGCCTGGGCTGCAGCCTGCTCTTCATCCCC CTGGGCTTGGTAGACAGAAGGACGCACGCGCCGACGGTGCTGGCGCTGGGCTTCATGGCACTCTACTCCC TCCTGCCACACAAGGAGCTACGCTTCATCATCTATGCCTTCCCCATGCTCAACATCACGGCTGCCAGAGG CTGCTCCTACCTGCTGAATAACTATAAAAAGTCTTGGCTGTACAAAGCGGGGTCTCTGCTTGTGATCGGA CACCTCGTGGTGAATGCCGCCTACTCAGCCACGGCCCTGTATGTGTCCCATTTCAACTACCCAGGTGGCG TCGCAATGCAGAGGCTGCACCAGCTGGTGCCCCCCCAGACAGACGTCCTTCTGCACGTTGACGTGGCAGC CGCCCAGACAGGTGTGTCTCGGTTTCTCCAAGTCAACAGCGCCTGGAGGTACGACAAGAGGGAGGATGTG CAGCCGGGGACAGGCATGCTGGCATACACACACATCCTCATGGAGGCGGCCCCTGGGCTCCTGGCCCTCT ACAGGGACACACACCGGGTCCTGGCCAGCGTCGTGGGGACCACAGGTGTGAGTCTGAACCTGACCCAACT GCCCCCCTTCAACGTCCACCTGCAGACAAAGCTGGTGCTTCTGGAGAGGCTCCCCCGGCCGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215501 protein sequence
Red=Cloning site Green=Tags(s) MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLG PVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHL MFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRAL RHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIP LGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIG HLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHVDVAAAQTGVSRFLQVNSAWRYDKREDV QPGTGMLAYTHILMEAAPGLLALYRDTHRVLASVVGTTGVSLNLTQLPPFNVHLQTKLVLLERLPRPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024105 |
| ORF Size | 1464 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024105.4 |
| RefSeq Size | 2387 bp |
| RefSeq ORF | 1467 bp |
| Locus ID | 79087 |
| UniProt ID | Q9BV10 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 54.6 kDa |
| Gene Summary | This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215501L3 | Lenti ORF clone of Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215501L4 | Lenti ORF clone of Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12), mGFP tagged |
CNY 5,890.00 |
|
| RG215501 | ALG12 (tGFP-tagged) - Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12) |
CNY 4,370.00 |
|
| SC112248 | ALG12 (untagged)-Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12) |
CNY 2,950.00 |
