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GAB2 (NM_012296) Human Tagged ORF Clone
CAT＃: RC215374
GAB2 (Myc-DDK-tagged)-Human GRB2-associated binding protein 2 (GAB2), transcript variant 2
ORF Plasmid: tGFP
"NM_012296" in other vectors (6)
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CNY 7,424.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215374 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCGGCGGCGACGTGGTGTGCACCGGCTGGCTGAGGAAATCGCCTCCCGAGAAGAAGTTGAGGC GCTATGCCTGGAAGAAACGCTGGTTTATCCTGCGGAGTGGCCGGATGAGCGGTGACCCAGATGTTCTGGA ATACTACAAGAACGATCACTCCAAGAAGCCTCTGCGGATCATCAACCTGAACTTCTGTGAGCAGGTAGAT GCAGGCCTGACCTTTAACAAGAAGGAGCTGCAGGATAGTTTTGTGTTTGACATCAAGACCAGTGAACGCA CCTTTTACCTGGTGGCTGAGACAGAAGAGGACATGAATAAGTGGGTCCAGAGCATCTGCCAGATCTGTGG CTTCAATCAGGCTGAGGAGAGCACAGACTCCCTGAGAAATGTTTCCTCAGCCGGTCATGGCCCCCGCTCT TCTCCAGCTGAGCTCAGCAGCTCTAGCCAGCACCTTCTCCGAGAGCGCAAGTCCTCAGCCCCATCACACT CCAGCCAGCCAACTCTGTTCACGTTTGAACCCCCTGTGTCAAACCACATGCAGCCCACCTTGTCCACCAG CGCACCTCAGGAGTATCTCTACTTGCACCAGTGCATAAGCCGAAGAGCAGAAAATGCAAGGAGTGCCAGC TTCTCTCAGGGCACCAGAGCCTCTTTTCTCATGAGGAGTGACACAGCTGTACAAAAACTTGCCCAGGGCA ATGGACACTGTGTCAACGGGATCAGTGGTCAAGTCCATGGCTTCTATAGCCTTCCCAAGCCGAGCCGGCA CAATACAGAATTCAGAGACAGTACCTACGACCTCCCCCGCAGCCTGGCCTCCCATGGCCACACCAAGGGC AGCCTCACAGGCTCCGAGACAGATAATGAGGATGTGTACACCTTCAAGACGCCCAGCAACACCCTGTGCA GGGAGTTCGGGGACCTCCTGGTAGACAATATGGATGTTCCGGCCACCCCACTCTCAGCCTACCAGATCCC TAGGACATTCACTCTGGACAAAAACCACAATGCCATGACAGTGGCCACTCCTGGGGACTCAGCCATAGCT CCCCCACCCCGCCCCCCCAAGCCAAGTCAGGCAGAAACACCTCGATGGGGCAGTCCTCAGCAGAGACCGC CAATCAGTGAAAATAGCAGATCTGTCGCTGCCACCATCCCCAGACGCAACACCCTCCCTGCAATGGACAA CAGCCGACTTCACCGAGCTTCTTCCTGTGAGACCTACGAGTACCCACAGCGTGGTGGAGAGAGTGCAGGC CGGTCTGCTGAATCCATGAGTGATGGAGTTGGCTCTTTCCTGCCAGGGAAAATGATTGTGGGCCGATCGG ACAGCACCAATTCTGAAGACAACTATGTGCCCATGAATCCAGGTTCTTCCACCCTGTTGGCCATGGAACG AGCAGGTGATAATTCCCAGAGCGTCTACATCCCAATGAGCCCAGGGGCCCATCACTTTGACTCACTTGGC TACCCATCAACAACCCTTCCTGTGCACCGAGGCCCCAGCAGAGGAAGTGAGATTCAGCCACCCCCTGTCA ACCGCAACCTCAAACCTGATCGGAAAGCAAAGCCAACACCACTTGACCTGAGGAACAACACCGTCATCGA TGAACTCCCCTTCAAGTCACCTATCACCAAGTCTTGGTCTAGGGCCAACCACACCTTCAACTCCAGCTCC TCCCAGTACTGCCGCCCCATCTCCACCCAGAGCATCACCAGCACAGACTCAGGAGACAGCGAAGAGAACT ATGTCCCTATGCAAAACCCAGTGTCTGCATCTCCCGTTCCCAGTGGCACGAACAGTCCTGCCCCTAAGAA GAGCACCGGCAGCGTTGATTATCTGGCCCTGGACTTCCAGCCGAGCTCCCCAAGCCCCCACCGCAAGCCA TCTACTTCATCCGTCACCTCTGATGAGAAGGTGGACTACGTTCAGGTGGACAAGGAGAAGACCCAGGCCC TGCAGAACACCATGCAGGAGTGGACAGACGTGCGGCAGTCCTCAGAGCCTTCCAAGGGTGCCAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215374 protein sequence
Red=Cloning site Green=Tags(s) MSGGGDVVCTGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPLRIINLNFCEQVD AGLTFNKKELQDSFVFDIKTSERTFYLVAETEEDMNKWVQSICQICGFNQAEESTDSLRNVSSAGHGPRS SPAELSSSSQHLLRERKSSAPSHSSQPTLFTFEPPVSNHMQPTLSTSAPQEYLYLHQCISRRAENARSAS FSQGTRASFLMRSDTAVQKLAQGNGHCVNGISGQVHGFYSLPKPSRHNTEFRDSTYDLPRSLASHGHTKG SLTGSETDNEDVYTFKTPSNTLCREFGDLLVDNMDVPATPLSAYQIPRTFTLDKNHNAMTVATPGDSAIA PPPRPPKPSQAETPRWGSPQQRPPISENSRSVAATIPRRNTLPAMDNSRLHRASSCETYEYPQRGGESAG RSAESMSDGVGSFLPGKMIVGRSDSTNSEDNYVPMNPGSSTLLAMERAGDNSQSVYIPMSPGAHHFDSLG YPSTTLPVHRGPSRGSEIQPPPVNRNLKPDRKAKPTPLDLRNNTVIDELPFKSPITKSWSRANHTFNSSS SQYCRPISTQSITSTDSGDSEENYVPMQNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKP STSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012296 |
| ORF Size | 2028 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6062 bp |
| RefSeq ORF | 1917 bp |
| Locus ID | 9846 |
| UniProt ID | Q9UQC2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chronic myeloid leukemia, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis |
| MW | 74.5 kDa |
| Gene Summary | This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215374L1 | Lenti ORF clone of Human GRB2-associated binding protein 2 (GAB2), transcript variant 2, Myc-DDK-tagged |
CNY 9,824.00 |
|
| RC215374L2 | Lenti ORF clone of Human GRB2-associated binding protein 2 (GAB2), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC215374L3 | Lenti ORF clone of Human GRB2-associated binding protein 2 (GAB2), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC215374L4 | Lenti ORF clone of Human GRB2-associated binding protein 2 (GAB2), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG215374 | GAB2 (tGFP-tagged) - Human GRB2-associated binding protein 2 (GAB2), transcript variant 2 |
CNY 9,024.00 |
|
| SC303953 | GAB2 (untagged)-Human GRB2-associated binding protein 2 (GAB2), transcript variant 2 |
CNY 7,608.00 |
