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PEG3 (NM_006210) Human Tagged ORF Clone
CAT＃: RC215328
PEG3 (Myc-DDK-tagged)-Human paternally expressed 3 (PEG3), transcript variant 1
ORF Plasmid: tGFP
"NM_006210" in other vectors (6)
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CNY 16,296.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PW1; ZKSCAN22; ZNF904; ZSCAN24 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215328 representing NM_006210
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCTCCAAAGCACTTGTCTGCCACCAAACCTAAGAAGTCCTGGGCCCCAAATCTGTATGAGCTAG ACAGTGACTTGACTAAGGAGCCGGATGTCATCATAGGAGAAGGTCCAACTGACTCTGAGTTTTTTCATCA GAGGTTTCGGAACCTAATCTATGTGGAATTTGTTGGGCCTCGGAAGACCCTGATCAAACTCCGAAACCTC TGCCTCGATTGGTTGCAGCCGGAGACCCGCACCAAGGAGGAGATCATCGAGCTCTTGGTCCTTGAGCAGT ACCTGACCATCATCCCTGAAAAGCTCAAGCCTTGGGTGCGAGCAAAAAAGCCGGAGAACTGTGAGAAGCT CGTCACTCTGCTGGAGAATTACAAGGAGATGTACCAACCAGAAGACGACAACAACAGTGACGTGACCAGC GACGACGACATGACCCGGAACAGAAGAGAGTCCTCACCACCTCACTCAGTCCATTCTTTCAGTGACCGGG ACTGGGACCGGAGGGGCAGAAGCAGAGACATGGAGCCACGAGACCGCTGGTCCCACACCAGGAACCCAAG AAGCAGGATGCCTCCGCGGGATCTTTCCCTTCCTGTGGTGGCGAAAACAAGCTTTGAAATGGACAGAGAG GACGACAGGGACTCCAGGGCTTATGAGTCCCGATCTCAGGATGCTGAATCATACCAAAATGTGGTGGACC TCGCTGAGGACAGGAAACCTCACAACACAATCCAGGACAACATGGAAAACTACAGGAAGCTGCTCTCCCT CGTGCAGCTTGCTGAAGACGATGGCCACTCCCACATGACGCAGGGCCACTCATCAAGATCCAAGAGAAGT GCCTACCCAAGCACCAGTCGAGGTCTAAAAACTATGCCTGAAGCCAAAAAATCAACCCACCGGCGGGGGA TTTGTGAAGATGAATCTTCCCACGGAGTGATAATGGAAAAATTCATCAAGGATGTGTCACGCAGTTCCAA ATCGGGAAGAGCAAGGGAGTCAAGCGACCGGTCACAGAGATTCCCCAGAATGTCAGATGATAACTGGAAG GACATTTCATTGAACAAGAGGGAGTCAGTGATCCAGCAGCGGGTTTATGAAGGGAATGCATTTAGGGGAG GCTTTAGGTTTAATTCAACCCTTGTTTCCAGAAAGAGAGTTCTTGAAAGAAAGAGGCGCTATCATTTTGA CACAGATGGGAAGGGCTCGATTCACGATCAAAAAGGCTGTCCCAGGAAGAAGCCCTTTGAATGTGGTAGT GAGATGAGAAAAGCCATGAGCGTGAGCAGCCTGAGCAGCCTCAGCTCCCCCTCCTTTACCGAGTCACAGC CAATTGATTTTGGGGCAATGCCATATGTATGTGATGAGTGTGGGAGGTCGTTCAGTGTCATCTCAGAATT TGTTGAGCACCAGATCATGCATACTAGAGAGAACCTCTATGAGTATGGTGAGTCCTTTATCCACAGTGTG GCTGTCAGTGAAGTTCAGAAAAGTCAGGTTGGAGGGAAACGTTTTGAATGTAAGGACTGTGGAGAGACCT TCAATAAGAGTGCCGCCTTGGCTGAACATCGGAAGATTCATGCTAGAGGTTATCTTGTGGAATGTAAGAA TCAGGAATGTGAGGAAGCCTTCATGCCTAGCCCCACCTTTAGTGAGCTTCAGAAAATATATGGCAAAGAC AAATTCTACGAGTGCAGGGTGTGTAAGGAAACCTTCCTTCATAGTTCTGCCCTGATTGAGCACCAGAAAA TCCACTTTGGGGATGACAAAGATAATGAGCGTGAACATGAACGTGAACGTGAACGTGAGCGCGGGGAAAC CTTTAGGCCCAGCCCAGCCCTTAATGAGTTTCAGAAAATGTATGGTAAAGAGAAAATGTACGAATGTAAG GTGTGTGGGGAGACTTTCCTTCATAGCTCATCCCTGAAAGAACATCAGAAAATCCATACTAGAGGGAACC CATTTGAAAACAAGGGTAAAGTGTGTGAGGAAACCTTTATTCCTGGTCAGTCCCTTAAAAGGCGTCAGAA AACTTACAATAAGGAGAAGCTCTGTGACTTTACAGATGGCCGGGATGCCTTCATGCAAAGCTCAGAGCTC AGTGAGCATCAGAAAATTCATTCTCGAAAGAACCTCTTTGAAGGCAGAGGGTATGAGAAATCTGTCATTC ATAGTGGGCCATTCACTGAATCTCAGAAGAGTCATACTATAACAAGACCTCTTGAAAGTGATGAGGACGA AAAGGCGTTCACCATTAGCTCTAACCCCTATGAAAACCAGAAGATTCCCACTAAGGAAAATGTCTATGAG GCAAAATCATATGAGAGGTCTGTTATTCATAGCTTAGCCTCTGTGGAAGCTCAGAAAAGTCACAGTGTAG CAGGGCCCAGTAAACCAAAAGTAATGGCAGAGTCTACCATTCAGAGCTTCGATGCTATCAACCATCAGAG AGTTCGTGCTGGAGGGAACACCTCTGAAGGAAGGGAATACAGTAGGTCTGTTATCCATAGCTTAGTGGCT TCCAAACCTCCAAGAAGTCACAATGGAAATGAATTGGTGGAATCTAATGAGAAGGGAGAATCCTCCATTT ATATCTCAGACCTTAATGATAAGCGACAGAAGATTCCTGCCAGAGAGAACCCTTGTGAAGGGGGCAGTAA GAATCGCAACTATGAAGACTCTGTCATACAGAGTGTATTCCGTGCCAAACCTCAGAAAAGTGTTCCTGGA GAGGGATCTGGTGAGTTTAAGAAGGATGGCGAATTCTCTGTTCCCAGCTCAAATGTCCGTGAATACCAGA AGGCTCGTGCTAAAAAGAAATACATTGAGCATAGGAGCAATGAGACCTCTGTAATTCACTCTCTGCCTTT TGGTGAACAAACATTTCGCCCTCGAGGGATGCTCTATGAATGTCAGGAGTGTGGGGAGTGCTTTGCTCAT AGCTCTGACCTCACTGAGCACCAGAAGATTCATGATAGGGAGAAGCCCTCTGGAAGCAGAAACTATGAAT GGTCTGTCATTCGCAGCTTGGCCCCTACTGACCCTCAAACAAGTTACGCCCAAGAGCAGTATGCTAAAGA GCAAGCGCGGAACAAATGTAAGGACTTCAGACAATTTTTTGCTACCAGCGAAGACCTCAACACAAACCAG AAAATCTATGACCAAGAGAAGTCTCATGGCGAGGAGTCTCAAGGCGAGAATACTGATGGGGAGGAGACCC ACAGCGAGGAGACCCATGGTCAGGAGACAATTGAAGACCCTGTCATTCAAGGCTCAGACATGGAAGACCC TCAGAAGGATGACCCTGATGACAAAATCTATGAATGTGAGGACTGTGGCCTGGGCTTTGTGGATCTCACA GACCTCACAGACCATCAGAAAGTCCACAGCAGGAAGTGCCTGGTTGACAGTCGGGAGTACACACATTCTG TAATTCACACCCATTCCATCAGCGAGTATCAGAGAGATTACACTGGAGAGCAGCTGTATGAATGTCCAAA GTGTGGGGAATCTTTTATTCATAGCTCATTCCTTTTCGAGCATCAGAGAATCCATGAACAAGACCAGTTG TATTCCATGAAGGGGTGTGATGATGGTTTTATTGCCCTCTTGCCCATGAAGCCACGGAGGAATCGTGCTG CAGAGAGGAATCCTGCTCTTGCTGGGTCGGCCATTCGATGCCTTTTGTGTGGACAAGGCTTCATTCATAG CTCTGCCCTTAATGAGCATATGAGACTTCATAGGGAAGATGATTTACTGGAGCAGAGCCAGATGGCTGAG GAAGCTATCATTCCAGGCTTAGCCCTCACTGAGTTTCAGAGAAGTCAGACCGAAGAGAGACTCTTTGAAT GTGCAGTCTGTGGAGAATCTTTCGTCAACCCAGCAGAACTTGCAGATCACGTAACTGTTCATAAGAATGA GCCCTATGAGTACGGGTCCTCCTATACTCACACCTCATTTCTTACTGAGCCCCTCAAAGGAGCTATACCA TTCTATGAATGCAAGGATTGTGGTAAGTCCTTTATTCATAGCACAGTCCTCACTAAACATAAGGAGCTTC ATCTGGAAGAAGAAGAAGAAGATGAAGCAGCAGCAGCTGCAGCAGCAGCAGCCCAGGAAGTTGAAGCCAA TGTCCATGTTCCACAAGTAGTTCTGAGGATTCAGGGCTTAAACGTAGAGGCTGCTGAGCCAGAAGTGGAG GCTGCCGAGCCAGAAGTGGAGGCTGCTGAGCCAGAAGTGGAGGCTGCTGAGCCAAACGGAGAGGCTGAAG GGCCAGATGGAGAGGCTGCAGAGCCCATTGGAGAGGCTGGACAGCCAAATGGAGAGGCCGAGCAGCCAAA TGGGGATGCTGATGAGCCAGATGGTGCAGGTATTGAAGACCCAGAAGAAAGAGCTGAAGAGCCAGAGGGA AAAGCTGAAGAGCCAGAGGGAGATGCCGACGAGCCTGACGGTGTGGGAATTGAAGACCCAGAAGAAGGTG AAGATCAAGAGATTCAGGTAGAAGAACCATACTATGACTGCCATGAATGCACAGAAACCTTCACTTCCAG CACAGCATTCAGTGAACACCTGAAAACTCATGCCAGCATGATCATATTTGAGCCTGCAAATGCCTTTGGG GAGTGCTCAGGCTACATCGAACGTGCCAGCACCAGCACAGGTGGTGCCAATCAAGCTGATGAGAAGTACT TCAAATGTGACGTCTGTGGGCAGCTCTTCAATGACCGCCTGTCCCTCGCCAGACACCAGAATACCCACAC TGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215328 representing NM_006210
Red=Cloning site Green=Tags(s) MLPPKHLSATKPKKSWAPNLYELDSDLTKEPDVIIGEGPTDSEFFHQRFRNLIYVEFVGPRKTLIKLRNL CLDWLQPETRTKEEIIELLVLEQYLTIIPEKLKPWVRAKKPENCEKLVTLLENYKEMYQPEDDNNSDVTS DDDMTRNRRESSPPHSVHSFSDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLSLPVVAKTSFEMDRE DDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLVQLAEDDGHSHMTQGHSSRSKRS AYPSTSRGLKTMPEAKKSTHRRGICEDESSHGVIMEKFIKDVSRSSKSGRARESSDRSQRFPRMSDDNWK DISLNKRESVIQQRVYEGNAFRGGFRFNSTLVSRKRVLERKRRYHFDTDGKGSIHDQKGCPRKKPFECGS EMRKAMSVSSLSSLSSPSFTESQPIDFGAMPYVCDECGRSFSVISEFVEHQIMHTRENLYEYGESFIHSV AVSEVQKSQVGGKRFECKDCGETFNKSAALAEHRKIHARGYLVECKNQECEEAFMPSPTFSELQKIYGKD KFYECRVCKETFLHSSALIEHQKIHFGDDKDNEREHERERERERGETFRPSPALNEFQKMYGKEKMYECK VCGETFLHSSSLKEHQKIHTRGNPFENKGKVCEETFIPGQSLKRRQKTYNKEKLCDFTDGRDAFMQSSEL SEHQKIHSRKNLFEGRGYEKSVIHSGPFTESQKSHTITRPLESDEDEKAFTISSNPYENQKIPTKENVYE AKSYERSVIHSLASVEAQKSHSVAGPSKPKVMAESTIQSFDAINHQRVRAGGNTSEGREYSRSVIHSLVA SKPPRSHNGNELVESNEKGESSIYISDLNDKRQKIPARENPCEGGSKNRNYEDSVIQSVFRAKPQKSVPG EGSGEFKKDGEFSVPSSNVREYQKARAKKKYIEHRSNETSVIHSLPFGEQTFRPRGMLYECQECGECFAH SSDLTEHQKIHDREKPSGSRNYEWSVIRSLAPTDPQTSYAQEQYAKEQARNKCKDFRQFFATSEDLNTNQ KIYDQEKSHGEESQGENTDGEETHSEETHGQETIEDPVIQGSDMEDPQKDDPDDKIYECEDCGLGFVDLT DLTDHQKVHSRKCLVDSREYTHSVIHTHSISEYQRDYTGEQLYECPKCGESFIHSSFLFEHQRIHEQDQL YSMKGCDDGFIALLPMKPRRNRAAERNPALAGSAIRCLLCGQGFIHSSALNEHMRLHREDDLLEQSQMAE EAIIPGLALTEFQRSQTEERLFECAVCGESFVNPAELADHVTVHKNEPYEYGSSYTHTSFLTEPLKGAIP FYECKDCGKSFIHSTVLTKHKELHLEEEEEDEAAAAAAAAAQEVEANVHVPQVVLRIQGLNVEAAEPEVE AAEPEVEAAEPEVEAAEPNGEAEGPDGEAAEPIGEAGQPNGEAEQPNGDADEPDGAGIEDPEERAEEPEG KAEEPEGDADEPDGVGIEDPEEGEDQEIQVEEPYYDCHECTETFTSSTAFSEHLKTHASMIIFEPANAFG ECSGYIERASTSTGGANQADEKYFKCDVCGQLFNDRLSLARHQNTHTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006210 |
| ORF Size | 4764 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006210.3 |
| RefSeq Size | 6371 bp |
| RefSeq ORF | 4767 bp |
| Locus ID | 5178 |
| UniProt ID | Q9GZU2 |
| Protein Families | Transcription Factors |
| MW | 180.6 kDa |
| Gene Summary | In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215328L1 | Lenti ORF clone of Human paternally expressed 3 (PEG3), transcript variant 1, Myc-DDK-tagged |
CNY 18,696.00 |
|
| RC215328L2 | Lenti ORF clone of Human paternally expressed 3 (PEG3), transcript variant 1, mGFP tagged |
CNY 17,770.00 |
|
| RC215328L3 | Lenti ORF clone of Human paternally expressed 3 (PEG3), transcript variant 1, Myc-DDK-tagged |
CNY 17,770.00 |
|
| RC215328L4 | Lenti ORF clone of Human paternally expressed 3 (PEG3), transcript variant 1, mGFP tagged |
CNY 17,770.00 |
|
| RG215328 | PEG3 (tGFP-tagged) - Human paternally expressed 3 (PEG3), transcript variant 1 |
CNY 17,896.00 |
|
| SC128281 | PEG3 (untagged)-Human paternally expressed 3 (PEG3), transcript variant 1 |
CNY 16,304.00 |
