United States
Germany
Japan
United Kingdom
China
Kv4.2 (KCND2) (NM_012281) Human Tagged ORF Clone
CAT＃: RC215266
KCND2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2)
ORF Plasmid: tGFP
"NM_012281" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KV4.2; RK5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215266 representing NM_012281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGTGGCAGCGTGGCTGCCTTTTGCAAGGGCAGCGGCTATCGGGTGGATGCCTGTGGCCT CGGGGCCTATGCCGGCTCCCCCGAGGCAGGAGAGGAAAAGGACCCAAGATGCTCTCATTGTGCTGAATGT GAGTGGCACCCGCTTCCAGACGTGGCAGGACACCCTGGAACGTTACCCAGACACTCTACTGGGCAGTTCT GAGAGGGACTTTTTCTACCACCCAGAAACTCAGCAGTATTTCTTTGACCGTGACCCAGACATCTTCCGCC ACATCCTGAATTTCTACCGCACTGGGAAGCTCCACTATCCTCGCCACGAGTGCATCTCTGCTTACGATGA AGAACTGGCCTTCTTTGGCCTCATCCCGGAAATCATCGGCGACTGCTGTTATGAGGAGTACAAGGATCGC AGGCGAGAGAACGCCGAGCGCCTGCAGGACGACGCGGATACCGACACCGCTGGGGAGAGCGCCTTGCCCA CCATGACTGCAAGGCAGAGGGTCTGGAGGGCCTTCGAGAACCCCCACACCAGCACGATGGCCCTGGTGTT CTACTATGTCACGGGGTTTTTCATTGCCGTCTCTGTCATCGCGAATGTGGTGGAAACAGTGCCGTGCGGA TCAAGCCCAGGTCACATTAAAGAACTGCCCTGTGGAGAGCGGTATGCTGTGGCCTTCTTCTGCTTGGACA CGGCCTGCGTCATGATCTTCACAGTTGAGTATTTGCTTCGCCTGGCTGCAGCGCCTAGTCGTTACCGTTT TGTGCGTAGTGTCATGAGTATCATCGACGTGGTGGCCATCCTGCCTTATTACATTGGGCTGGTGATGACA GACAATGAGGACGTCAGCGGAGCCTTTGTCACACTCCGAGTCTTCCGGGTCTTCAGGATCTTTAAGTTTT CCCGCCACTCTCAAGGCCTGCGCATCCTGGGGTACACACTGAAGAGTTGTGCCTCAGAATTGGGCTTCTT GCTTTTCTCGCTCACCATGGCTATCATCATCTTCGCTACAGTTATGTTCTACGCAGAGAAGGGGTCTTCG GCTAGCAAGTTCACCAGCATCCCTGCAGCCTTCTGGTATACCATCGTCACCATGACAACACTAGGGTATG GTGACATGGTGCCAAAAACCATAGCAGGGAAGATTTTTGGTTCTATCTGTTCGCTGAGTGGGGTCTTGGT CATTGCTCTACCTGTTCCGGTGATTGTATCCAACTTCAGTCGCATCTACCACCAGAATCAACGAGCAGAC AAACGAAGGGCACAAAAGAAAGCTAGACTGGCCAGGATCCGGGCAGCCAAAAGCGGAAGCGCAAATGCTT ACATGCAGAGCAAACGGAATGGTTTACTCAGTAATCAGCTGCAGTCCTCAGAGGATGAGCAGGCTTTTGT TAGCAAATCCGGCTCCAGCTTTGAAACCCAGCACCACCACCTGCTTCACTGCCTGGAAAAAACCACGAAT CACGAGTTTGTGGACGAACAAGTCTTTGAAGAAAGCTGCATGGAAGTTGCAACTGTTAATCGTCCTTCAA GTCACAGTCCTTCACTGTCTTCACAACAAGGAGTCACCAGCACCTGCTGTTCACGACGACACAAAAAAAC TTTTCGCATCCCAAATGCCAATGTATCAGGAAGCCATCAAGGTAGTATACAAGAACTCAGCACGATTCAG ATCAGATGTGTGGAGAGAACACCTCTGTCTAACAGCCGATCCAGTTTAAATGCCAAAATGGAAGAGTGTG TTAAACTAAACTGTGAACAACCTTATGTGACTACAGCAATAATAAGCATCCCAACACCTCCAGTAACCAC ACCAGAAGGAGACGATAGGCCAGAATCCCCTGAGTACTCAGGAGGAAATATTGTCAGAGTTTCTGCTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215266 representing NM_012281
Red=Cloning site Green=Tags(s) MAAGVAAWLPFARAAAIGWMPVASGPMPAPPRQERKRTQDALIVLNVSGTRFQTWQDTLERYPDTLLGSS ERDFFYHPETQQYFFDRDPDIFRHILNFYRTGKLHYPRHECISAYDEELAFFGLIPEIIGDCCYEEYKDR RRENAERLQDDADTDTAGESALPTMTARQRVWRAFENPHTSTMALVFYYVTGFFIAVSVIANVVETVPCG SSPGHIKELPCGERYAVAFFCLDTACVMIFTVEYLLRLAAAPSRYRFVRSVMSIIDVVAILPYYIGLVMT DNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSS ASKFTSIPAAFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRAD KRRAQKKARLARIRAAKSGSANAYMQSKRNGLLSNQLQSSEDEQAFVSKSGSSFETQHHHLLHCLEKTTN HEFVDEQVFEESCMEVATVNRPSSHSPSLSSQQGVTSTCCSRRHKKTFRIPNANVSGSHQGSIQELSTIQ IRCVERTPLSNSRSSLNAKMEECVKLNCEQPYVTTAIISIPTPPVTTPEGDDRPESPEYSGGNIVRVSAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012281 |
| ORF Size | 1890 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012281.3 |
| RefSeq Size | 5333 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 3751 |
| UniProt ID | Q9NZV8 |
| Domains | BTB, K_tetra, ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 70.4 kDa |
| Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member mediates a rapidly inactivating, A-type outward potassium current which is not under the control of the N terminus as it is in Shaker channels. [provided by RefSeq, Jul 2008] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of Kv4.2 protein complex and modifications by tandem affinity purification-mass spectrometry in primary neurons
,null,
Frontiers in Cellular Neuroscience
,PubMed ID 36568885
[Kv4.2]
|
|
P38 Regulates Kainic Acid-Induced Seizure and Neuronal Firing via Kv4.2 Phosphorylation
,Hu, JH;Malloy, C;Hoffman, DA;,
Int J Mol Sci
,PubMed ID 32824677
[Kv4.2]
|
|
Activity-dependent isomerization of Kv4.2 by Pin1 regulates cognitive flexibility
,Hu, JH;Malloy, C;Tabor, GT;Gutzmann, JJ;Liu, Y;Abebe, D;Karlsson, RM;Durell, S;Cameron, HA;Hoffman, DA;,
Nat Commun
,PubMed ID 32218435
[Kv4.2]
|
|
A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking
,Tabor, GT;Park, JM;Murphy, JG;Hu, JH;Hoffman, DA;,
Mol. Cell. Neurosci.
,PubMed ID 31212013
[Kv4.2]
|
|
A polybasic motif in alternatively spliced KChIP2 isoforms prevents Ca2+ regulation of Kv4 channels
,Murphy, JG;Hoffman, DA;,
J. Biol. Chem.
,PubMed ID 30622142
[Kv4.2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215266L1 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215266L2 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2), mGFP tagged |
CNY 5,890.00 |
|
| RC215266L3 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215266L4 | Lenti ORF clone of Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2), mGFP tagged |
CNY 5,890.00 |
|
| RG215266 | KCND2 (tGFP-tagged) - Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2) |
CNY 4,370.00 |
|
| SC115436 | KCND2 (untagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 2 (KCND2) |
CNY 4,696.00 |
