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Insulin Receptor (INSR) (NM_001079817) Human Tagged ORF Clone
CAT＃: RC215257
- TrueORF®
INSR (Myc-DDK-tagged)-Human insulin receptor (INSR), transcript variant 2
ORF Plasmid: tGFP
"NM_001079817" in other vectors (6)
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CNY 14,184.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD220; HHF5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215257 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGGCCACCGGGGGCCGGCGGGGGGCGGCGGCCGCGCCGCTGCTGGTGGCGGTGGCCGCGCTGCTACT GGGCGCCGCGGGCCACCTGTACCCCGGAGAGGTGTGTCCCGGCATGGATATCCGGAACAACCTCACTAGG TTGCATGAGCTGGAGAATTGCTCTGTCATCGAAGGACACTTGCAGATACTCTTGATGTTCAAAACGAGGC CCGAAGATTTCCGAGACCTCAGTTTCCCCAAACTCATCATGATCACTGATTACTTGCTGCTCTTCCGGGT CTATGGGCTCGAGAGCCTGAAGGACCTGTTCCCCAACCTCACGGTCATCCGGGGATCACGACTGTTCTTT AACTACGCGCTGGTCATCTTCGAGATGGTTCACCTCAAGGAACTCGGCCTCTACAACCTGATGAACATCA CCCGGGGTTCTGTCCGCATCGAGAAGAACAATGAGCTCTGTTACTTGGCCACTATCGACTGGTCCCGTAT CCTGGATTCCGTGGAGGATAATTACATCGTGTTGAACAAAGATGACAACGAGGAGTGTGGAGACATCTGT CCGGGTACCGCGAAGGGCAAGACCAACTGCCCCGCCACCGTCATCAACGGGCAGTTTGTCGAACGATGTT GGACTCATAGTCACTGCCAGAAAGTTTGCCCGACCATCTGTAAGTCACACGGCTGCACCGCCGAAGGCCT CTGTTGCCACAGCGAGTGCCTGGGCAACTGTTCTCAGCCCGACGACCCCACCAAGTGCGTGGCCTGCCGC AACTTCTACCTGGACGGCAGGTGTGTGGAGACCTGCCCGCCCCCGTACTACCACTTCCAGGACTGGCGCT GTGTGAACTTCAGCTTCTGCCAGGACCTGCACCACAAATGCAAGAACTCGCGGAGGCAGGGCTGCCACCA GTACGTCATTCACAACAACAAGTGCATCCCTGAGTGTCCCTCCGGGTACACGATGAATTCCAGCAACTTG CTGTGCACCCCATGCCTGGGTCCCTGTCCCAAGGTGTGCCACCTCCTAGAAGGCGAGAAGACCATCGACT CGGTGACGTCTGCCCAGGAGCTCCGAGGATGCACCGTCATCAACGGGAGTCTGATCATCAACATTCGAGG AGGCAACAATCTGGCAGCTGAGCTAGAAGCCAACCTCGGCCTCATTGAAGAAATTTCAGGGTATCTAAAA ATCCGCCGATCCTACGCTCTGGTGTCACTTTCCTTCTTCCGGAAGTTACGTCTGATTCGAGGAGAGACCT TGGAAATTGGGAACTACTCCTTCTATGCCTTGGACAACCAGAACCTAAGGCAGCTCTGGGACTGGAGCAA ACACAACCTCACCATCACTCAGGGGAAACTCTTCTTCCACTATAACCCCAAACTCTGCTTGTCAGAAATC CACAAGATGGAAGAAGTTTCAGGAACCAAGGGGCGCCAGGAGAGAAACGACATTGCCCTGAAGACCAATG GGGACCAGGCATCCTGTGAAAATGAGTTACTTAAATTTTCTTACATTCGGACATCTTTTGACAAGATCTT GCTGAGATGGGAGCCGTACTGGCCCCCCGACTTCCGAGACCTCTTGGGGTTCATGCTGTTCTACAAAGAG GCCCCTTATCAGAATGTGACGGAGTTCGACGGGCAGGATGCGTGTGGTTCCAACAGTTGGACGGTGGTAG ACATTGACCCACCCCTGAGGTCCAACGACCCCAAATCACAGAACCACCCAGGGTGGCTGATGCGGGGTCT CAAGCCCTGGACCCAGTATGCCATCTTTGTGAAGACCCTGGTCACCTTTTCGGATGAACGCCGGACCTAT GGGGCCAAGAGTGACATCATTTATGTCCAGACAGATGCCACCAACCCCTCTGTGCCCCTGGATCCAATCT CAGTGTCTAACTCATCATCCCAGATTATTCTGAAGTGGAAACCACCCTCCGACCCCAATGGCAACATCAC CCACTACCTGGTTTTCTGGGAGAGGCAGGCGGAAGACAGTGAGCTGTTCGAGCTGGATTATTGCCTCAAA GGGCTGAAGCTGCCCTCGAGGACCTGGTCTCCACCATTCGAGTCTGAAGATTCTCAGAAGCACAACCAGA GTGAGTATGAGGATTCGGCCGGCGAATGCTGCTCCTGTCCAAAGACAGACTCTCAGATCCTGAAGGAGCT GGAGGAGTCCTCGTTTAGGAAGACGTTTGAGGATTACCTGCACAACGTGGTTTTCGTCCCCAGAAAAACC TCTTCAGGCACTGGTGCCGAGGACCCTAGGCCATCTCGGAAACGCAGGTCCCTTGGCGATGTTGGGAATG TGACGGTGGCCGTGCCCACGGTGGCAGCTTTCCCCAACACTTCCTCGACCAGCGTGCCCACGAGTCCGGA GGAGCACAGGCCTTTTGAGAAGGTGGTGAACAAGGAGTCGCTGGTCATCTCCGGCTTGCGACACTTCACG GGCTATCGCATCGAGCTGCAGGCTTGCAACCAGGACACCCCTGAGGAACGGTGCAGTGTGGCAGCCTACG TCAGTGCGAGGACCATGCCTGAAGCCAAGGCTGATGACATTGTTGGCCCTGTGACGCATGAAATCTTTGA GAACAACGTCGTCCACTTGATGTGGCAGGAGCCGAAGGAGCCCAATGGTCTGATCGTGCTGTATGAAGTG AGTTATCGGCGATATGGTGATGAGGAGCTGCATCTCTGCGTCTCCCGCAAGCACTTCGCTCTGGAACGGG GCTGCAGGCTGCGTGGGCTGTCACCGGGGAACTACAGCGTGCGAATCCGGGCCACCTCCCTTGCGGGCAA CGGCTCTTGGACGGAACCCACCTATTTCTACGTGACAGACTATTTAGACGTCCCGTCAAATATTGCAAAA ATTATCATCGGCCCCCTCATCTTTGTCTTTCTCTTCAGTGTTGTGATTGGAAGTATTTATCTATTCCTGA GAAAGAGGCAGCCAGATGGGCCGCTGGGACCGCTTTACGCTTCTTCAAACCCTGAGTATCTCAGTGCCAG TGATGTGTTTCCATGCTCTGTGTACGTGCCGGACGAGTGGGAGGTGTCTCGAGAGAAGATCACCCTCCTT CGAGAGCTGGGGCAGGGCTCCTTCGGCATGGTGTATGAGGGCAATGCCAGGGACATCATCAAGGGTGAGG CAGAGACCCGCGTGGCGGTGAAGACGGTCAACGAGTCAGCCAGTCTCCGAGAGCGGATTGAGTTCCTCAA TGAGGCCTCGGTCATGAAGGGCTTCACCTGCCATCATGTGGTGCGCCTCCTGGGAGTGGTGTCCAAGGGC CAGCCCACGCTGGTGGTGATGGAGCTGATGGCTCACGGAGACCTGAAGAGCTACCTCCGTTCTCTGCGGC CAGAGGCTGAGAATAATCCTGGCCGCCCTCCCCCTACCCTTCAAGAGATGATTCAGATGGCGGCAGAGAT TGCTGACGGGATGGCCTACCTGAACGCCAAGAAGTTTGTGCATCGGGACCTGGCAGCGAGAAACTGCATG GTCGCCCATGATTTTACTGTCAAAATTGGAGACTTTGGAATGACCAGAGACATCTATGAAACGGATTACT ACCGGAAAGGGGGCAAGGGTCTGCTCCCTGTACGGTGGATGGCACCGGAGTCCCTGAAGGATGGGGTCTT CACCACTTCTTCTGACATGTGGTCCTTTGGCGTGGTCCTTTGGGAAATCACCAGCTTGGCAGAACAGCCT TACCAAGGCCTGTCTAATGAACAGGTGTTGAAATTTGTCATGGATGGAGGGTATCTGGATCAACCCGACA ACTGTCCAGAGAGAGTCACTGACCTCATGCGCATGTGCTGGCAATTCAACCCCAAGATGAGGCCAACCTT CCTGGAGATTGTCAACCTGCTCAAGGACGACCTGCACCCCAGCTTTCCAGAGGTGTCGTTCTTCCACAGC GAGGAGAACAAGGCTCCCGAGAGTGAGGAGCTGGAGATGGAGTTTGAGGACATGGAGAATGTGCCCCTGG ACCGTTCCTCGCACTGTCAGAGGGAGGAGGCGGGGGGCCGGGATGGAGGGTCCTCGCTGGGTTTCAAGCG GAGCTACGAGGAACACATCCCTTACACACACATGAACGGAGGCAAGAAAAACGGGCGGATTCTGACCTTG CCTCGGTCCAATCCTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215257 protein sequence
Red=Cloning site Green=Tags(s) XWPPGAGGGRRPRRCWWRWPRCYWAPRATCTPERCVPAWISGTTSLGCMSWRIALSSKDTCRYS*CSKRG PKISETSVSPNSS*SLITCCSSGSMGSRA*RTCSPTSRSSGDHDCSLTTRWSSSRWFTSRNSASTT**TS PGVLSASRRTMSSVTWPLSTGPVSWIPWRIITSC*TKMTTRSVETSVRVPRRARPTAPPPSSTGSLSNDV GLIVTARKFARPSVSHTAAPPKASVATASAWATVLSPTTPPSAWPAATSTWTAGVWRPARPRTTTSRTGA V*TSASARTCTTNARTRGGRAATSTSFTTTSASLSVPPGTR*IPATCCAPHAWVPVPRCATS*KARRPST R*RLPRSSEDAPSSTGV*SSTFEEATIWQLS*KPTSASLKKFQGI*KSADPTLWCHFPSSGSYV*FEERP WKLGTTPSMPWTTRT*GSSGTGANTTSPSLRGNSSSTITPNSACQKSTRWKKFQEPRGARRETTLP*RPM GTRHPVKMSYLNFLTFGHLLTRSC*DGSRTGPPTSETSWGSCCSTKRPLIRM*RSSTGRMRVVPTVGRW* TLTHP*GPTTPNHRTTQGG*CGVSSPGPSMPSL*RPWSPFRMNAGPMGPRVTSFMSRQMPPTPLCPWIQS QCLTHHPRLF*SGNHPPTPMATSPTTWFSGRGRRKTVSCSSWIIASKG*SCPRGPGLHHSSLKILRSTTR VSMRIRPANAAPVQRQTLRS*RSWRSPRLGRRLRITCTTWFSSPEKPLQALVPRTLGHLGNAGPLAMLGM *RWPCPRWQLSPTLPRPACPRVRRSTGLLRRW*TRSRWSSPACDTSRAIASSCRLATRTPLRNGAVWQPT SVRGPCLKPRLMTLLAL*RMKSLRTTSST*CGRSRRSPMV*SCCMK*VIGDMVMRSCISASPASTSLWNG AAGCVGCHRGTTACESGPPPLRATALGRNPPIST*QTI*TSRQILQKLSSAPSSLSFSSVL*LEVFIYS* ERGSQMGRWDRFTLLQTLSISVPVMCFHALCTCRTSGRCLERRSPSFESWGRAPSAWCMRAMPGTSSRVR QRPAWR*RRSTSQPVSESGLSSSMRPRS*RASPAIMWCASWEWCPRASPRWW*WS*WLTET*RATSVLCG QRLRIILAALPLPFKR*FRWRQRLLTGWPT*TPRSLCIGTWQRETAWSPMILLSKLETLE*PETSMKRIT TGKGARVCSLYGGWHRSP*RMGSSPLLLTCGPLAWSFGKSPAWQNSLTKACLMNRC*NLSWMEGIWINPT TVQRESLTSCACAGNSTPR*GQPSWRLSTCSRTTCTPAFQRCRSSTARRTRLPRVRSWRWSLRTWRMCPW TVPRTVRGRRRGAGMEGPRWVSSGATRNTSLTHT*TEARKTGGF*PCLGPIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001079817 |
| ORF Size | 4146 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001079817.1, NM_001079817.2, NP_001073285.1 |
| RefSeq Size | 9023 bp |
| RefSeq ORF | 4113 bp |
| Locus ID | 3643 |
| UniProt ID | P06213 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Insulin signaling pathway, Type II diabetes mellitus |
| MW | 156.3 kDa |
| Gene Summary | This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215257L1 | Lenti-ORF clone of INSR (Myc-DDK-tagged)-Human insulin receptor (INSR), transcript variant 2 |
CNY 16,584.00 |
|
| RC215257L2 | Lenti-ORF clone of INSR (mGFP-tagged)-Human insulin receptor (INSR), transcript variant 2 |
CNY 7,600.00 |
|
| RC215257L3 | Lenti-ORF clone of INSR (Myc-DDK-tagged)-Human insulin receptor (INSR), transcript variant 2 |
CNY 7,600.00 |
|
| RC215257L4 | Lenti-ORF clone of INSR (mGFP-tagged)-Human insulin receptor (INSR), transcript variant 2 |
CNY 16,584.00 |
|
| RG215257 | INSR (tGFP-tagged) - Human insulin receptor (INSR), transcript variant 2 |
CNY 15,784.00 |
|
| SC315880 | INSR (untagged)-Human insulin receptor (INSR), transcript variant 2 |
CNY 14,072.00 |
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