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ETNK2 (NM_018208) Human Tagged ORF Clone
CAT＃: RC214974
- TrueORF®
ETNK2 (Myc-DDK-tagged)-Human ethanolamine kinase 2 (ETNK2)
ORF Plasmid: tGFP
"NM_018208" in other vectors (4)
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CNY 8,460.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EKI2; HMFT1716 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214974 representing NM_018208
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGCCCCCTTCGGCCCCTCAGCCGCGCGCGTCCTTTCACCTGAGGAGGCACACGCCTTGCCCGC AGTGCTCATGGGGCATGGAGGAGAAGGCGGCGGCCAGCGCCAGCTGCCGGGAGCCGCCGGGCCCCCCGAG GGCCGCCGCCGTCGCGTACTTCGGCATTTCCGTGGACCCGGACGACATCCTTCCCGGGGCCCTGCGCCTC ATCCAGGAGCTGCGGCCGCATTGGAAACCCGAGCAAGTTCGGACCAAGCGCTTCACGGATGGCATCACCA ACAAGCTGGTGGCCTGCTATGTGGAGGAGGACATGCAGGACTGCGTGCTGGTCCGGGTGTATGGGGAGCG GACGGAGCTGCTGGTGGACCGGGAGAATGAGGTCAGAAACTTCCAGCTGCTGCGAGCACACAGCTGTGCC CCCAAACTCTACTGCACCTTCCAGAATGGGCTGTGCTATGAGTACATGCAGGGTGTGGCCCTGGAGCCTG AGCACATCCGTGAGCCCCGGCTTTTCAGGTTAATCGCCTTAGAAATGGCAAAGATTCATACTATCCACGC CAACGGCAGCCTGCCCAAGCCCATCCTCTGGCACAAGATGCACAATTATTTCACGCTTGTGAAGAACGAG ATCAACCCCAGCCTTTCTGCAGATGTCCCTAAGGTAGAGGTGTTGGAACGGGAGCTGGCCTGGCTGAAGG AGCATCTGTCCCAGCTGGAGTCCCCTGTGGTGTTTTGTCACAATGACCTGCTCTGCAAGAATATCATCTA TGACAGCATCAAAGGTCACGTGCGGTTCATTGACTATGAATATGCTGGCTACAACTACCAAGCTTTTGAC ATTGGCAACCATTTCAATGAGTTTGCAGGCGTGAATGAGGTGGATTACTGCCTGTACCCGGCGCGGGAGA CCCAGCTGCAGTGGCTGCACTACTACCTGCAGACACAAAAGGGGATGGCCGTGACCCCCAGGGAGGTGCA AAGGCTCTACGTGCAAGTCAACAAGTTTGCCCTGGCGTCTCACTTCTTCTGGGCTCTCTGGGCCCTCATC CAGAACCAGTACTCCACCATCGACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214974 representing NM_018208
Red=Cloning site Green=Tags(s) MAVPPSAPQPRASFHLRRHTPCPQCSWGMEEKAAASASCREPPGPPRAAAVAYFGISVDPDDILPGALRL IQELRPHWKPEQVRTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTELLVDRENEVRNFQLLRAHSCA PKLYCTFQNGLCYEYMQGVALEPEHIREPRLFRLIALEMAKIHTIHANGSLPKPILWHKMHNYFTLVKNE INPSLSADVPKVEVLERELAWLKEHLSQLESPVVFCHNDLLCKNIIYDSIKGHVRFIDYEYAGYNYQAFD IGNHFNEFAGVNEVDYCLYPARETQLQWLHYYLQTQKGMAVTPREVQRLYVQVNKFALASHFFWALWALI QNQYSTID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018208 |
| ORF Size | 2537 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2430 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 55224 |
| UniProt ID | Q9NVF9 |
| Domains | Choline_kinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
| MW | 41.54 kDa |
| Gene Summary | The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hepatic metastasis of gastric cancer is associated with enhanced expression of ethanolamine kinase 2 via the p53-Bcl-2 intrinsic apoptosis pathway
,Miwa, T;Kanda, M;Shimizu, D;Umeda, S;Sawaki, K;Tanaka, H;Tanaka, C;Hattori, N;Hayashi, M;Yamada, S;Nakayama, G;Koike, M;Kodera, Y;,
British journal of cancer
,PubMed ID 33531692
[ETNK2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214974L3 | Lenti-ORF clone of ETNK2 (Myc-DDK-tagged)-Human ethanolamine kinase 2 (ETNK2) |
CNY 10,360.00 |
|
| RC214974L4 | Lenti-ORF clone of ETNK2 (mGFP-tagged)-Human ethanolamine kinase 2 (ETNK2) |
CNY 10,360.00 |
|
| RG214974 | ETNK2 (tGFP-tagged) - Human ethanolamine kinase 2 (ETNK2) |
CNY 9,310.00 |
|
| SC113646 | ETNK2 (untagged)-Human ethanolamine kinase 2 (ETNK2) |
CNY 3,656.00 |
