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ZFYVE16 (NM_014733) Human Tagged ORF Clone
CAT＃: RC214827
- TrueORF®
ZFYVE16 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1
ORF Plasmid: tGFP
"NM_014733" in other vectors (4)
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CNY 16,848.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PPP1R69 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214827 representing NM_014733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAACTCCTTGATGATTTTGAACAGAACCCAG ATGAACAAGATTATCTCCAAGATGTACAAAATGCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTT GGCTTCCTCACAGCGAACTTCATTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAA ACAAGCTATGGAACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATG AAAAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATCCAGCCGTT ATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGTAACTTAGTTCATGCAACC AATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTTAAGTCTAATGCAGATTCCTTGATTGGAT TGGATTTATCTTCAGTGTCAGATACTCCCTGTGTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGA ACAACAGAATGATATCAGTTCTGAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAA GTAGATACAACACTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTA ATCAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAAATGTTTCA TGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGATGTTGGCTTAGTAAAAGAG GAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAAGAAGAGGGCAAGACAAGTGCTTTGACCT GCAGCCTTCCGAAAAATGAAGATTTATGCTTAAATGATTCAAATTCAAGAGATGAAAATTTCAAATTACC TGACTTTTCCTTTCAGGAAGATAAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGAC CTTAAGGATAATGATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCT CATTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGGGGTGATTT TTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACATGAAGAAATACAGAACAGT GTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTGAAACAGGAAAAATGTAAAAGCATACTCC TTCAGTCATTAATTGAAGGGATGGAAGACAGAAAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTC TTTGGATGGTGGTGACACCAGTTCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCA GAGTCTTCTGATTGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAG ATTACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGATGCCTTTCT GACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTAAATGACTCTAAATCGCAA ATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATCAATAATATATATTTCAATGCAGAAGCAG GAGCTATTGGGGAAAGTCATGGTATTAATATAATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAA TGGCCTTTCTTTAGGAGAAAAAAGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATT ACAAATCAATTATCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTA GCCTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATAGAAAGTGA ACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACAGCTGATCCACAGGTTAGC TTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGTGGATCTAGTTTCGTAACTGCAAATGAAG ATTCTGTACCTGAAAACACTTGCAAAGAAGGCTTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGA TTCAGAAGCTCCAAACTGTATGAACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGA GCATGTGGGAAAGTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAG CAAGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGCTCAGGCATTTGAAAGGATGATGAGTCCAAC TGGTTCTAATCTTAAGTCTAATCATTCTGATGAATGTACTACTGTCCAGCCTCCTCAGGAGAACCAAACA TCCAGTATACCTTCACCAGCAACTTTGCCAGTCTCAGCACTTAAACAACCAGGTGTTGAAGGACTATGTT CCAAAGAACAGAAGAGAGTATGGTTTGCAGATGGTATATTGCCCAATGGTGAAGTTGCAGATACAACAAA ATTATCATCTGGAAGTAAAAGATGTTCTGAAGACTTTAGTCCTCTCTCACCTGATGTGCCTATGACAGTA AACACAGTGGATCATTCCCATTCTACTACAGTGGAAAAGCCAAACAATGAGACAGGAGATATTACAAGAA ATGAGATAATTCAGAGTCCTATTTCTCAGGTTCCATCAGTGGAAAAATTGTCTATGAACACAGGAAATGA GGGGTTACCTACTTCTGGTTCATTTACACTAGATGATGATGTTTTTGCAGAAACTGAAGAACCATCTAGT CCTACTGGTGTCTTAGTTAACAGCAATTTACCTATTGCTAGTATTTCAGATTATAGGTTACTGTGTGATA TTAACAAGTATGTCTGCAATAAGATTAGTCTTCTACCTAATGATGAGGACAGTTTGCCCCCACTTCTGGT TGCATCTGGAGAAAAGGGATCAGTGCCTGTAGTAGAAGAACATCCATCTCATGAGCAGATCATTTTGCTT CTTGAAGGTGAAGGCTTTCATCCTGTTACATTTGTCCTAAATGCTAATCTACTCGTGAATGTCAAATTCA TATTTTATTCCTCAGACAAATATTGGTACTTTTCAACCAATGGATTGCATGGCTTGGGACAGGCAGAAAT TATTATTCTATTGTTATGTTTGCCAAATGAAGATACTATTCCTAAGGACATCTTCAGACTATTTATCACC ATATATAAGGATGCTCTAAAAGGAAAATACATAGAAAACTTGGACAATATTACCTTTACTGAGAGTTTTC TCAGTAGCAAGGATCACGGAGGATTCCTGTTTATTACACCTACTTTTCAGAAACTTGATGATCTCTCATT ACCAAGTAATCCTTTTCTTTGTGGAATTCTTATCCAGAAGCTTGAGATTCCCTGGGCAAAGGTTTTTCCT ATGCGTTTAATGTTGAGATTGGGTGCAGAATATAAAGCATATCCTGCTCCTCTAACAAGCATCAGAGGCC GAAAACCTCTTTTTGGAGAAATAGGACACACTATTATGAACTTACTTGTTGACCTTCGAAATTACCAGTA TACCTTGCATAATATAGATCAACTGTTGATTCATATGGAAATGGGAAAAAGCTGCATAAAAATACCACGG AAAAAGTACAGTGATGTAATGAAAGTACTAAATTCTTCCAATGAGCATGTCATTAGCATTGGAGCAAGTT TCAGTACAGAAGCAGATTCTCATCTAGTCTGTATACAGAATGATGGAATTTATGAAACACAGGCCAACAG TGCCACTGGCCATCCTAGAAAAGTGACAGGTGCAAGTTTTGTGGTATTCAATGGAGCTCTAAAAACATCT TCAGGATTTCTTGCTAAGTCCAGCATAGTTGAAGATGGCTTAATGGTACAAATAACTCCAGAGACCATGA ATGGCTTGCGGCTAGCTTTACGAGAACAGAAAGACTTTAAAATTACATGTGGGAAAGTTGATGCAGTAGA CCTGAGAGAATACGTGGATATCTGCTGGGTAGATGCTGAAGAAAAAGGAAACAAAGGAGTTATCAGTTCA GTGGATGGAATATCATTACAAGGATTTCCAAGTGAAAAAATAAAACTGGAAGCAGATTTTGAAACCGATG AGAAGATTGTAAAATGTACCGAGGTGTTCTACTTTCTAAAGGACCAGGATTTATCTATTTTATCAACTTC TTATCAGTTTGCAAAAGAAATAGCCATGGCTTGTAGTGCTGCGCTGTGCCCTCACCTGAAAACTCTAAAA AGTAATGGGATGAATAAAATTGGACTCAGAGTTTCCATTGACACTGATATGGTTGAATTTCAGGCAGGAT CTGAAGGCCAACTTCTGCCTCAGCATTATCTAAATGATCTTGATAGTGCTCTGATACCTGTGATCCATGG TGGGACCTCCAACTCTAGTTTACCATTAGAAATAGAATTAGTGTTTTTCATTATAGAACATCTTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214827 representing NM_014733
Red=Cloning site Green=Tags(s) MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSE TSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHAT NSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDISSELQNREIGGIKELGIK VDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKE EVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLD LKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVP ESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQ MNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVDKQNTIENGLSLGEKSTIPVQQGLPTSKSEI TNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVS FNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCR ACGKVFCGVCCNRKCKLQYLEKEARVCVVCYETISKAQAFERMMSPTGSNLKSNHSDECTTVQPPQENQT SSIPSPATLPVSALKQPGVEGLCSKEQKRVWFADGILPNGEVADTTKLSSGSKRCSEDFSPLSPDVPMTV NTVDHSHSTTVEKPNNETGDITRNEIIQSPISQVPSVEKLSMNTGNEGLPTSGSFTLDDDVFAETEEPSS PTGVLVNSNLPIASISDYRLLCDINKYVCNKISLLPNDEDSLPPLLVASGEKGSVPVVEEHPSHEQIILL LEGEGFHPVTFVLNANLLVNVKFIFYSSDKYWYFSTNGLHGLGQAEIIILLLCLPNEDTIPKDIFRLFIT IYKDALKGKYIENLDNITFTESFLSSKDHGGFLFITPTFQKLDDLSLPSNPFLCGILIQKLEIPWAKVFP MRLMLRLGAEYKAYPAPLTSIRGRKPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGKSCIKIPR KKYSDVMKVLNSSNEHVISIGASFSTEADSHLVCIQNDGIYETQANSATGHPRKVTGASFVVFNGALKTS SGFLAKSSIVEDGLMVQITPETMNGLRLALREQKDFKITCGKVDAVDLREYVDICWVDAEEKGNKGVISS VDGISLQGFPSEKIKLEADFETDEKIVKCTEVFYFLKDQDLSILSTSYQFAKEIAMACSAALCPHLKTLK SNGMNKIGLRVSIDTDMVEFQAGSEGQLLPQHYLNDLDSALIPVIHGGTSNSSLPLEIELVFFIIEHLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014733 |
| ORF Size | 4617 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014733.5 |
| RefSeq Size | 6280 bp |
| RefSeq ORF | 4620 bp |
| Locus ID | 9765 |
| Domains | FYVE |
| Protein Pathways | TGF-beta signaling pathway |
| MW | 168.7 kDa |
| Gene Summary | This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214827L3 | Lenti-ORF clone of ZFYVE16 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1 |
CNY 17,290.00 |
|
| RC214827L4 | Lenti-ORF clone of ZFYVE16 (mGFP-tagged)-Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1 |
CNY 17,290.00 |
|
| RG214827 | ZFYVE16 (tGFP-tagged) - Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1 |
CNY 18,448.00 |
|
| SC114882 | ZFYVE16 (untagged)-Human zinc finger, FYVE domain containing 16 (ZFYVE16), transcript variant 1 |
CNY 10,536.00 |
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