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PPP1R9B (NM_032595) Human Tagged ORF Clone
CAT＃: RC213696
PPP1R9B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B)
ORF Plasmid: tGFP
"NM_032595" in other vectors (7)
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CNY 8,976.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PPP1R6; PPP1R9; SPINO; Spn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213696 representing NM_032595
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGACGGAGCCACGGGGGCCCGGGGGTCCCCTCCGGAGCGCCTCCCCGCACCGCAGCGCCTACG AGGCGGGCATCCAGGCGCTGAAGCCGCCCGACGCGCCCGGGCCCGACGAGGCACCCAAGGGGGCCCACCA CAAGAAATATGGCTCCAACGTCCACCGCATCAAAAGTATGTTCCTGCAGATGGGCACGACGGCGGGGCCC TCGGGCGAGGCGGGCGGCGGCGCGGGCCTGGCCGAGGCCCCACGGGCGTCCGAGCGCGGCGTGCGCCTGT CGCTGCCGCGGGCCAGCAGCCTGAACGAGAACGTGGACCACAGCGCCCTGCTGAAGCTGGGCACCAGCGT GTCGGAGCGCGTGAGCCGCTTCGACTCCAAGCCCGCGCCCTCCGCGCAGCCTGCGCCGCCGCCGCACCCG CCGTCCCGGCTGCAGGAGACGCGGAAGCTGTTCGAACGGAGCGCCCCAGCGGCCGCAGGCGGCGACAAGG AGGCCGCGGCGCGGCGGCTGCTGAGGCAGGAGCGCGCCGGCCTGCAGGACCGGAAGCTGGACGTCGTGGT GCGCTTCAACGGCAGCACCGAGGCGCTGGACAAGCTGGACGCTGACGCCGTGTCCCCCACGGTCAGCCAG CTCAGCGCCGTCTTCGAGAAGGCCGACTCGAGGACCGGCCTCCACCGCGGGCCCGGGCTCCCCAGGGCCG CAGGGGTTCCCCAGGTCAACTCGAAGCTGGTCAGCAAGCGGTCCCGGGTGTTCCAGCCCCCGCCGCCGCC GCCGCCCGCCCCGTCGGGGGATGCCCCGGCCGAGAAAGAGCGATGCCCCGCAGGGCAGCAGCCCCCGCAG CACCGAGTGGCCCCTGCCCGGCCGCCCCCCAAGCCCCGGGAGGTGCGCAAGATTAAGCCGGTGGAGGTGG AGGAGAGCGGGGAGTCGGAGGCCGAGTCGGCGCCCGGGGAGGTGATCCAGGCCGAGGTTACGGTCCACGC GGCCCTGGAGAATGGCAGCACCGTGGCAACTGCAGCCAGCCCCGCGCCCGAGGAGCCAAAGGCCCAAGCG GCCCCGGAGAAGGAGGCGGCGGCGGTAGCGCCGCCAGAGAGGGGGGTGGGCAATGGCCGGGCCCCGGACG TGGCCCCTGAGGAGGTAGATGAATCCAAGAAGGAGGACTTCTCGGAGGCGGACTTGGTGGACGTGAGCGC CTACAGTGGGCTCGGGGAGGACTCTGCGGGCAGTGCCCTGGAGGAGGACGACGAAGACGACGAGGAGGAT GGGGAGCCCCCCTACGAGCCCGAGTCGGGGTGCGTGGAGATCCCGGGGCTGTCGGAGGAGGAGGACCCAG CCCCGAGCCGGAAGATCCATTTCAGCACGGCGCCCATCCAAGTGTTCAGCACTTACTCCAACGAGGATTA CGATCGTCGCAACGAGGATGTGGATCCCATGGCAGCCTCTGCTGAGTACGAGCTGGAGAAGCGTGTGGAG AGGTTGGAGCTGTTCCCTGTGGAGCTGGAGAAGGACTCCGAGGGCCTGGGCATCAGCATCATCGGCATGG GCGCCGGGGCAGACATGGGCCTGGAGAAGCTGGGTATCTTCGTCAAGACCGTGACGGAGGGTGGTGCGGC CCATCGGGATGGCAGGATCCAGGTGAATGATCTCCTGGTGGAGGTGGATGGAACAAGTCTGGTGGGAGTG ACCCAGAGCTTCGCGGCGTCTGTGCTCCGGAACACCAAGGGCCGAGTGCGGTTTATGATTGGCCGGGAGC GGCCGGGAGAGCAGAGCGAAGTGGCCCAGCTAATTCAGCAGACTTTGGAACAGGAGCGATGGCAGCGGGA GATGATGGAGCAGAGATACGCCCAGTATGGGGAGGATGACGAGGAGACGGGAGAGTATGCCACTGACGAG GATGAGGAGCTGAGCCCCACGTTCCCGGGTGGTGAGATGGCCATCGAGGTGTTTGAGCTAGCGGAGAACG AGGATGCACTGTCCCCTGTGGACATGGAGCCCGAGAAGCTGGTGCACAAGTTCAAGGAGCTCCAGATCAA GCATGCGGTCACTGAGGCAGAGATCCAGCAGCTGAAAAGAAAGCTGCAGAGCCTGGAGCAGGAGAAGGGG CGCTGGCGGGTGGAGAAGGCGCAGTTGGAGCAGAGTGTGGAGGAGAACAAGGAGCGCATGGAGAAACTGG AAGGCTACTGGGGTGAGGCCCAGAGCCTGTGCCAGGCTGTGGACGAGCACCTGCGGGAGACTCAGGCGCA GTACCAGGCCCTGGAGCGCAAGTACAGCAAGGCCAAGCGCCTCATCAAGGACTACCAGCAGAAGGAGATC GAGTTCCTGAAAAAGGAGACTGCACAGCGTCGGGTTCTGGAGGAGTCGGAGCTGGCCAGAAAGGAGGAGA TGGACAAGCTCCTGGACAAGATCTCAGAACTGGAAGGAAACTTGCAAACACTGAGGAATTCCAATTCTAC T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213696 representing NM_032595
Red=Cloning site Green=Tags(s) MMKTEPRGPGGPLRSASPHRSAYEAGIQALKPPDAPGPDEAPKGAHHKKYGSNVHRIKSMFLQMGTTAGP SGEAGGGAGLAEAPRASERGVRLSLPRASSLNENVDHSALLKLGTSVSERVSRFDSKPAPSAQPAPPPHP PSRLQETRKLFERSAPAAAGGDKEAAARRLLRQERAGLQDRKLDVVVRFNGSTEALDKLDADAVSPTVSQ LSAVFEKADSRTGLHRGPGLPRAAGVPQVNSKLVSKRSRVFQPPPPPPPAPSGDAPAEKERCPAGQQPPQ HRVAPARPPPKPREVRKIKPVEVEESGESEAESAPGEVIQAEVTVHAALENGSTVATAASPAPEEPKAQA APEKEAAAVAPPERGVGNGRAPDVAPEEVDESKKEDFSEADLVDVSAYSGLGEDSAGSALEEDDEDDEED GEPPYEPESGCVEIPGLSEEEDPAPSRKIHFSTAPIQVFSTYSNEDYDRRNEDVDPMAASAEYELEKRVE RLELFPVELEKDSEGLGISIIGMGAGADMGLEKLGIFVKTVTEGGAAHRDGRIQVNDLLVEVDGTSLVGV TQSFAASVLRNTKGRVRFMIGRERPGEQSEVAQLIQQTLEQERWQREMMEQRYAQYGEDDEETGEYATDE DEELSPTFPGGEMAIEVFELAENEDALSPVDMEPEKLVHKFKELQIKHAVTEAEIQQLKRKLQSLEQEKG RWRVEKAQLEQSVEENKERMEKLEGYWGEAQSLCQAVDEHLRETQAQYQALERKYSKAKRLIKDYQQKEI EFLKKETAQRRVLEESELARKEEMDKLLDKISELEGNLQTLRNSNST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032595 |
| ORF Size | 2451 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032595.5 |
| RefSeq Size | 4071 bp |
| RefSeq ORF | 2454 bp |
| Locus ID | 84687 |
| UniProt ID | Q96SB3 |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 89.2 kDa |
| Gene Summary | This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells
,Verdugo-Sivianes, EM;Rojas, AM;Muñoz-Galván, S;Otero-Albiol, D;Carnero, A;,
Theranostics
,PubMed ID 33537097
[PPP1R9B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213696L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B), Myc-DDK-tagged |
CNY 11,376.00 |
|
| RC213696L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B), mGFP tagged |
CNY 11,376.00 |
|
| RC213696L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B), Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC213696L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B), mGFP tagged |
CNY 10,550.00 |
|
| RG213696 | PPP1R9B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B) |
CNY 10,576.00 |
|
| SC128059 | PPP1R9B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B) |
CNY 15,680.00 |
|
| SC318297 | PPP1R9B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 9B (PPP1R9B) |
CNY 15,680.00 |
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