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MGAT5 (NM_002410) Human Tagged ORF Clone
CAT＃: RC213548
- TrueORF®
MGAT5 (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5)
ORF Plasmid: tGFP
"NM_002410" in other vectors (6)
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CNY 5,432.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | glcNAc-T V; GNT-V; GNT-VA; MGAT5A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213548 representing NM_002410
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCTTCACTCCGTGGAAGTTGTCCTCTCAGAAGCTGGGCTTTTTCCTGGTGACTTTTGGCTTCA TTTGGGGTATGATGCTTCTGCACTTTACCATCCAGCAGCGAACTCAGCCTGAAAGCAGCTCCATGCTGCG CGAGCAGATCCTGGACCTCAGCAAAAGGTACATCAAGGCACTGGCAGAAGAAAACAGGAATGTGGTGGAT GGGCCATACGCTGGAGTCATGACAGCTTATGATCTGAAGAAAACCCTTGCTGTGTTATTAGATAACATTT TGCAGCGCATTGGCAAGTTGGAGTCGAAGGTGGACAATCTTGTTGTCAATGGCACCGGAACAAACTCAAC CAACTCCACTACAGCTGTTCCCAGCTTGGTTGCACTTGAGAAAATTAATGTGGCAGATATCATTAACGGA GCTCAAGAAAAATGTGTATTGCCTCCTATGGACGGCTACCCTCACTGTGAGGGAAAGATCAAGTGGATGA AAGACATGTGGCGTTCAGATCCCTGCTACGCAGACTATGGAGTGGATGGATCCACCTGCTCTTTTTTTAT TTACCTCAGTGAGGTTGAAAATTGGTGTCCTCATTTACCTTGGAGAGCAAAAAATCCCTACGAAGAAGCT GATCATAATTCATTGGCGGAAATTCGTACAGATTTTAATATTCTCTACAGTATGATGAAAAAGCATGAAG AATTCCGGTGGATGAGACTACGGATCCGGCGAATGGCTGACGCATGGATCCAAGCAATCAAGTCCCTGGC AGAAAAGCAGAACCTTGAAAAGAGAAAGCGGAAGAAAGTCCTCGTTCACCTGGGACTCCTGACCAAGGAA TCTGGATTTAAGATTGCAGAGACAGCTTTCAGTGGTGGCCCTCTTGGTGAATTAGTTCAATGGAGTGATT TAATTACATCTCTGTACTTACTGGGCCATGACATTAGGATTTCAGCTTCACTGGCTGAGCTCAAGGAAAT CATGAAGAAGGTTGTAGGAAACCGATCTGGCTGCCCAACTGTAGGAGACAGAATTGTTGAGCTCATTTAC ATTGATATTGTAGGACTTGCTCAATTCAAGAAAACTCTTGGACCATCCTGGGTTCATTACCAGTGCATGC TCCGAGTCCTTGATTCATTTGGTACTGAACCCGAATTTAATCATGCAAATTATGCCCAATCGAAAGGCCA CAAGACCCCTTGGGGAAAATGGAATCTGAACCCTCAGCAGTTTTATACCATGTTCCCTCATACCCCAGAC AACAGCTTTCTGGGGTTTGTGGTTGAGCAGCACCTGAACTCCAGTGATATCCACCACATTAATGAAATCA AAAGGCAGAACCAGTCCCTTGTGTATGGCAAAGTGGATAGCTTCTGGAAGAATAAGAAGATCTACTTGGA CATTATTCACACATACATGGAAGTGCATGCAACTGTTTATGGCTCCAGCACAAAGAATATTCCCAGTTAC GTGAAAAACCATGGTATCCTCAGTGGACGGGACCTGCAGTTCCTTCTTCGAGAAACCAAGTTGTTTGTTG GACTTGGGTTCCCTTACGAGGGCCCAGCTCCCCTGGAAGCTATCGCAAATGGATGTGCTTTTCTGAATCC CAAGTTCAACCCACCCAAAAGCAGCAAAAACACAGACTTTTTCATTGGCAAGCCAACTCTGAGAGAGCTG ACATCCCAGCATCCTTACGCTGAAGTTTTCATCGGGCGGCCACATGTGTGGACTGTTGACCTCAACAATC AGGAGGAAGTAGAGGATGCAGTGAAAGCAATTTTAAATCAGAAGATTGAGCCATACATGCCATATGAATT TACGTGCGAGGGGATGCTACAGAGAATCAATGCTTTCATTGAAAAACAGGACTTCTGCCATGGGCAAGTG ATGTGGCCACCCCTCAGCGCCCTACAGGTCAAGCTTGCTGAGCCCGGGCAGTCCTGCAAGCAGGTGTGCC AGGAGAGCCAGCTCATCTGCGAGCCTTCTTTCTTCCAGCACCTCAACAAGGACAAGGACATGCTGAAGTA CAAGGTGACCTGCCAAAGCTCAGAGCTGGCCAAGGACATCCTGGTGCCCTCCTTTGACCCTAAGAATAAG CACTGTGTGTTTCAAGGTGACCTCCTGCTCTTCAGCTGTGCAGGCGCCCACCCCAGGCACCAGAGGGTCT GCCCCTGCCGGGACTTCATCAAGGGCCAGGTGGCTCTCTGCAAAGACTGCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213548 representing NM_002410
Red=Cloning site Green=Tags(s) MALFTPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSSMLREQILDLSKRYIKALAEENRNVVD GPYAGVMTAYDLKKTLAVLLDNILQRIGKLESKVDNLVVNGTGTNSTNSTTAVPSLVALEKINVADIING AQEKCVLPPMDGYPHCEGKIKWMKDMWRSDPCYADYGVDGSTCSFFIYLSEVENWCPHLPWRAKNPYEEA DHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKE SGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIY IDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPD NSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSY VKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLREL TSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQV MWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNK HCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002410 |
| ORF Size | 2223 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002410.5 |
| RefSeq Size | 5078 bp |
| RefSeq ORF | 2226 bp |
| Locus ID | 4249 |
| UniProt ID | Q09328 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 84.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213548L1 | Lenti ORF clone of Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5), Myc-DDK-tagged |
CNY 7,832.00 |
|
| RC213548L2 | Lenti ORF clone of Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5), mGFP tagged |
CNY 7,700.00 |
|
| RC213548L3 | Lenti ORF clone of Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC213548L4 | Lenti ORF clone of Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5), mGFP tagged |
CNY 7,700.00 |
|
| RG213548 | MGAT5 (tGFP-tagged) - Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5) |
CNY 6,370.00 |
|
| SC314591 | MGAT5 (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5) |
CNY 5,432.00 |
