Tau (MAPT) (NM_005910) Human Tagged ORF Clone

CAT＃: RC213312

TrueORF^®

MAPT (Myc-DDK-tagged)-Human microtubule-associated protein tau (MAPT), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_005910" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00

货期*

现货

规格

Cited in 8 publications.

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA809201

MAPT mouse monoclonal antibody,clone OTI13B5

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; tau-40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213312 representing NM_005910 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGCCCCGCCAGGAGTTCGAAGTGATGGAAGATCACGCTGGGACGTACGGGTTGGGGGACAGGA AAGATCAGGGGGGCTACACCATGCACCAAGACCAAGAGGGTGACACGGACGCTGGCCTGAAAGAATCTCC CCTGCAGACCCCCACTGAGGACGGATCTGAGGAACCGGGCTCTGAAACCTCTGATGCTAAGAGCACTCCA ACAGCGGAAGATGTGACAGCACCCTTAGTGGATGAGGGAGCTCCCGGCAAGCAGGCTGCCGCGCAGCCCC ACACGGAGATCCCAGAAGGAACCACAGCTGAAGAAGCAGGCATTGGAGACACCCCCAGCCTGGAAGACGA AGCTGCTGGTCACGTGACCCAAGCTCGCATGGTCAGTAAAAGCAAAGACGGGACTGGAAGCGATGACAAA AAAGCCAAGGGGGCTGATGGTAAAACGAAGATCGCCACACCGCGGGGAGCAGCCCCTCCAGGCCAGAAGG GCCAGGCCAACGCCACCAGGATTCCAGCAAAAACCCCGCCCGCTCCAAAGACACCACCCAGCTCTGGTGA ACCTCCAAAATCAGGGGATCGCAGCGGCTACAGCAGCCCCGGCTCCCCAGGCACTCCCGGCAGCCGCTCC CGCACCCCGTCCCTTCCAACCCCACCCACCCGGGAGCCCAAGAAGGTGGCAGTGGTCCGTACTCCACCCA AGTCGCCGTCTTCCGCCAAGAGCCGCCTGCAGACAGCCCCCGTGCCCATGCCAGACCTGAAGAATGTCAA GTCCAAGATCGGCTCCACTGAGAACCTGAAGCACCAGCCGGGAGGCGGGAAGGTGCAGATAATTAATAAG AAGCTGGATCTTAGCAACGTCCAGTCCAAGTGTGGCTCAAAGGATAATATCAAACACGTCCCGGGAGGCG GCAGTGTGCAAATAGTCTACAAACCAGTTGACCTGAGCAAGGTGACCTCCAAGTGTGGCTCATTAGGCAA CATCCATCATAAACCAGGAGGTGGCCAGGTGGAAGTAAAATCTGAGAAGCTTGACTTCAAGGACAGAGTC CAGTCGAAGATTGGGTCCCTGGACAATATCACCCACGTCCCTGGCGGAGGAAATAAAAAGATTGAAACCC ACAAGCTGACCTTCCGCGAGAACGCCAAAGCCAAGACAGACCACGGGGCGGAGATCGTGTACAAGTCGCC AGTGGTGTCTGGGGACACGTCTCCACGGCATCTCAGCAATGTCTCCTCCACCGGCAGCATCGACATGGTA GACTCGCCCCAGCTCGCCACGCTAGCTGACGAGGTGTCTGCCTCCCTGGCCAAGCAGGGTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC213312 representing NM_005910 Red=Cloning site Green=Tags(s) MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTP TAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDK KAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRS RTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINK KLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRV QSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMV DSPQLATLADEVSASLAKQGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_005910
ORF Size	1323 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_005910.5
RefSeq Size	5731 bp
RefSeq ORF	1326 bp
Locus ID	4137
UniProt ID	P10636
Domains	tubulin-binding
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, MAPK signaling pathway
MW	45.7 kDa
Gene Summary	This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (8)

The use of this cDNA Clones has been cited in the following citations:
Truncating tau reveals different pathophysiological actions of oligomers in single neurons ,null, Communications Biology ,PubMed ID 34737403 [Tau]
Fyn kinase inhibition reduces protein aggregation, increases synapse density and improves memory in transgenic and traumatic Tauopathy ,Tang, SJ;Fesharaki-Zadeh, A;Takahashi, H;Nies, SH;Smith, LM;Luo, A;Chyung, A;Chiasseu, M;Strittmatter, SM;, Acta Neuropathol Commun ,PubMed ID 32611392 [Tau]
Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells ,Chadchankar, J;Korboukh, V;Conway, LC;Wobst, HJ;Walker, CA;Doig, P;Jacobsen, SJ;Brandon, NJ;Moss, SJ;Wang, Q;, PLoS ONE ,PubMed ID 31703099 [Tau]
Identification of a Novel Selective and Potent Inhibitor of Glycogen Synthase Kinase-3 ,Noori, MS;Bhatt, PM;Courreges, MC;Ghazanfari, D;Cuckler, C;Orac, CM;McMills, MC;Schwartz, FL;Deosarkar, SP;Bergmeier, SC;McCall, KD;Goetz, DJ;, Am. J. Physiol., Cell Physiol. ,PubMed ID 31553649 [Tau]
BIN1 favors the spreading of Tau via extracellular vesicles ,Crotti, A;Sait, HR;McAvoy, KM;Estrada, K;Ergun, A;Szak, S;Marsh, G;Jandreski, L;Peterson, M;Reynolds, TL;Dalkilic-Liddle, I;Cameron, A;Cahir-McFarland, E;Ransohoff, RM;, Sci Rep ,PubMed ID 31263146 [Tau]
Novel tau fragments in cerebrospinal fluid: relation to tangle pathology and cognitive decline in Alzheimer's disease ,Cicognola, C;Brinkmalm, G;Wahlgren, J;Portelius, E;Gobom, J;Cullen, NC;Hansson, O;Parnetti, L;Constantinescu, R;Wildsmith, K;Chen, HH;Beach, TG;Lashley, T;Zetterberg, H;Blennow, K;Höglund, K;, Acta Neuropathol. ,PubMed ID 30547227 [Tau]
Spatially-resolved intracellular sensing of hydrogen peroxide in living cells ,Warren, EA;Netterfield, TS;Sarkar, S;Kemp, ML;Payne, CK;, Sci Rep ,PubMed ID 26585385 [Tau]
Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400. ,null, FEBS letters ,PubMed ID 24113653 [Tau]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC213312L1	Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC213312L2	Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC213312L3	Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC213312L4	Lenti ORF clone of Human microtubule-associated protein tau (MAPT), transcript variant 2, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG213312	MAPT (tGFP-tagged) - Human microtubule-associated protein tau (MAPT), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
SC309606	MAPT (untagged)-Human microtubule-associated protein tau (MAPT), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,488.00

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