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LCT (NM_002299) Human Tagged ORF Clone
CAT＃: RC212751
- TrueORF®
LCT (Myc-DDK-tagged)-Human lactase (LCT)
ORF Plasmid: tGFP
"NM_002299" in other vectors (3)
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CNY 12,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LAC; LPH; LPH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212751 representing NM_002299
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTCTTGGCATGTAGTCTTTATTGCCCTGCTAAGTTTTTCATGCTGGGGGTCAGACTGGGAGT CTGATAGAAATTTCATTTCCACCGCTGGTCCTCTAACCAATGACTTGCTGCACAACCTGAGTGGTCTCCT GGGAGACCAGAGTTCTAACTTTGTAGCAGGGGACAAAGACATGTATGTTTGTCACCAGCCACTGCCCACT TTCCTGCCAGAATACTTCAGCAGTCTCCATGCCAGTCAGATCACCCATTATAAGGTATTTCTGTCATGGG CACAGCTCCTCCCAGCAGGAAGCACCCAGAATCCAGACGAGAAAACAGTGCAGTGCTACCGGCGACTCCT CAAGGCCCTCAAGACTGCACGGCTTCAGCCCATGGTCATCCTGCACCACCAGACCCTCCCTGCCAGCACC CTCCGGAGAACCGAAGCCTTTGCTGACCTCTTCGCCGACTATGCCACATTCGCCTTCCACTCCTTCGGGG ACCTAGTTGGGATCTGGTTCACCTTCAGTGACTTGGAGGAAGTGATCAAGGAGCTTCCCCACCAGGAATC AAGAGCGTCACAACTCCAGACCCTCAGTGATGCCCACAGAAAAGCCTATGAGATTTACCACGAAAGCTAT GCTTTTCAGGGCGGAAAACTCTCTGTTGTCCTGCGAGCTGAAGATATCCCGGAGCTCCTGCTAGAACCAC CCATATCTGCGCTTGCCCAGGACACGGTCGATTTCCTCTCTCTTGATTTGTCTTATGAATGCCAAAATGA GGCAAGTCTGCGGCAGAAGCTGAGTAAATTGCAGACCATTGAGCCAAAAGTGAAAGTTTTCATCTTCAAC CTAAAACTCCCAGACTGCCCCTCCACCATGAAGAACCCAGCCAGTCTGCTCTTCAGCCTTTTTGAAGCCA TAAATAAAGACCAAGTGCTCACCATTGGGTTTGATATTAATGAGTTTCTGAGTTGTTCATCAAGTTCCAA GAAAAGCATGTCTTGTTCTCTGACTGGCAGCCTGGCCCTTCAGCCTGACCAGCAGCAGGACCACGAGACC ACGGACTCCTCTCCTGCCTCTGCCTATCAGAGAATCTGGGAAGCATTTGCCAATCAGTCCAGGGCGGAAA GGGATGCCTTCCTGCAGGATACTTTCCCTGAAGGCTTCCTCTGGGGTGCCTCCACAGGAGCCTTTAACGT GGAAGGAGGCTGGGCCGAGGGTGGGAGAGGGGTGAGCATCTGGGATCCACGCAGGCCCCTGAACACCACT GAGGGCCAAGCGACGCTGGAGGTGGCCAGCGACAGTTACCACAAGGTAGCCTCTGACGTCGCCCTGCTTT GCGGCCTCCGGGCTCAGGTGTACAAGTTCTCCATCTCCTGGTCCCGGATCTTCCCCATGGGGCACGGGAG CAGCCCCAGCCTCCCAGGCGTTGCCTACTACAACAAGCTGATTGACAGGCTACAGGATGCGGGCATCGAG CCCATGGCCACGCTGTTCCACTGGGACCTGCCTCAGGCCCTGCAGGATCATGGTGGATGGCAGAATGAGA GCGTGGTGGATGCCTTCCTGGACTATGCGGCCTTCTGCTTCTCCACATTTGGGGACCGTGTGAAGCTGTG GGTGACCTTCCATGAGCCGTGGGTGATGAGCTACGCAGGCTATGGCACCGGCCAGCACCCTCCCGGCATC TCTGACCCAGGAGTGGCCTCTTTTAAGGTGGCTCACTTGGTCCTCAAGGCTCATGCCAGAACTTGGCACC ACTACAACAGCCATCATCGCCCACAGCAGCAGGGGCACGTGGGCATTGTGCTGAACTCAGACTGGGCAGA ACCCCTGTCTCCAGAGAGGCCTGAGGACCTGAGAGCCTCTGAGCGCTTCTTGCACTTCATGCTGGGCTGG TTTGCACACCCCGTCTTTGTGGATGGAGACTACCCAGCCACCCTGAGGACCCAGATCCAACAGATGAACA GACAGTGCTCCCATCCTGTGGCTCAACTCCCCGAGTTCACAGAGGCAGAGAAGCAGCTCCTGAAAGGCTC TGCTGATTTTCTGGGTCTGTCGCATTACACCTCCCGCCTCATCAGCAACGCCCCACAAAACACCTGCATC CCTAGCTATGATACCATTGGAGGCTTCTCCCAACACGTGAACCATGTGTGGCCCCAGACCTCATCCTCTT GGATTCGTGTGGTGCCCTGGGGGATAAGGAGGCTGTTGCAGTTTGTATCCCTGGAATACACAAGAGGAAA AGTTCCAATATACCTTGCCGGGAATGGCATGCCCATAGGGGAAAGTGAAAATCTCTTTGATGATTCCTTA AGAGTAGACTACTTCAATCAATATATCAATGAGGTGCTCAAGGCTATCAAGGAAGACTCTGTGGATGTTC GTTCCTACATTGCTCGTTCCCTCATTGATGGCTTCGAAGGCCCTTCTGGTTACAGCCAGCGGTTTGGCCT GCACCACGTCAACTTCAGCGACAGCAGCAAGTCAAGGACTCCCAGGAAATCTGCCTACTTTTTCACTAGC ATCATAGAAAAGAACGGTTTCCTCACCAAGGGGGCAAAAAGACTGCTACCACCTAATACAGTAAACCTCC CCTCCAAAGTCAGAGCCTTCACTTTTCCATCTGAGGTGCCCTCCAAGGCTAAAGTCGTTTGGGAAAAGTT CTCCAGCCAACCCAAGTTCGAAAGAGATTTGTTCTACCACGGGACGTTTCGGGATGACTTTCTGTGGGGC GTGTCCTCTTCCGCTTATCAGATTGAAGGCGCGTGGGATGCCGATGGCAAAGGCCCCAGCATCTGGGATA ACTTTACCCACACACCAGGGAGCAATGTGAAAGACAATGCCACTGGAGACATCGCCTGTGACAGCTATCA CCAGCTGGATGCCGATCTGAATATGCTCCGAGCTTTGAAGGTGAAGGCCTACCGCTTCTCTATCTCCTGG TCTCGGATTTTCCCAACTGGGAGAAACAGCTCTATCAACAGTCATGGGGTTGATTATTACAACAGGCTGA TCAATGGCTTGGTGGCAAGCAACATCTTTCCCATGGTGACATTGTTCCATTGGGACCTGCCCCAGGCCCT CCAGGATATCGGAGGCTGGGAGAATCCTGCCTTGATTGACTTGTTTGACAGCTACGCAGACTTTTGTTTC CAGACCTTTGGTGATAGAGTCAAGTTTTGGATGACTTTTAATGAGCCCATGTACCTGGCATGGCTAGGTT ATGGCTCAGGGGAATTTCCCCCAGGGGTGAAGGACCCAGGCTGGGCACCATATAGGATAGCCCACGCCGT CATCAAAGCCCATGCCAGAGTCTATCACACGTACGATGAGAAATACAGGCAGGAGCAGAAGGGGGTCATC TCGCTGAGCCTCAGTACACACTGGGCAGAGCCCAAGTCACCAGGGGTCCCCAGAGATGTGGAAGCCGCTG ACCGAATGCTGCAGTTCTCCCTGGGCTGGTTTGCTCACCCCATTTTTAGAAACGGAGACTATCCTGACAC CATGAAGTGGAAAGTGGGGAACAGGAGTGAACTGCAGCACTTAGCCACCTCCCGCCTGCCAAGCTTCACT GAGGAAGAGAAGAGGTTCATCAGGGCGACGGCCGACGTCTTCTGCCTCAACACGTACTACTCCAGAATCG TGCAGCACAAAACACCCAGGCTAAACCCACCCTCCTACGAAGACGACCAGGAGATGGCTGAGGAGGAGGA CCCTTCGTGGCCTTCCACGGCAATGAACAGAGCTGCGCCCTGGGGGACGCGAAGGCTGCTGAACTGGATC AAGGAAGAGTATGGTGACATCCCCATTTACATCACCGAAAACGGAGTGGGGCTGACCAATCCGAACACGG AGGATACTGATAGGATATTTTACCACAAAACCTACATCAATGAGGCTTTGAAAGCCTACAGGCTCGATGG TATAGACCTTCGAGGGTATGTCGCCTGGTCTCTGATGGACAACTTTGAGTGGCTAAATGGCTACACGGTC AAGTTTGGACTGTACCATGTTGATTTCAACAACACGAACAGGCCTCGCACAGCAAGAGCCTCCGCCAGGT ACTACACAGAGGTCATTACCAACAACGGCATGCCACTGGCCAGGGAGGATGAGTTTCTGTACGGACGGTT TCCTGAGGGCTTCATCTGGAGTGCAGCTTCTGCTGCATATCAGATTGAAGGTGCGTGGAGAGCAGATGGC AAAGGACTCAGCATTTGGGACACGTTTTCTCACACACCACTGAGGGTTGAGAACGATGCCATTGGAGACG TGGCCTGTGACAGTTATCACAAGATTGCTGAGGATCTGGTCACCCTGCAGAACCTGGGCGTGTCCCACTA CCGTTTTTCCATCTCCTGGTCTCGCATCCTCCCTGATGGAACCACCAGGTACATCAATGAAGCGGGCCTG AACTACTACGTGAGGCTCATCGATACACTGCTGGCCGCCAGCATCCAGCCCCAGGTGACCATTTACCACT GGGACCTACCACAGACGCTCCAAGATGTAGGAGGCTGGGAGAATGAGACCATCGTGCAGCGGTTTAAGGA GTATGCAGATGTGCTCTTCCAGAGGCTGGGAGACAAGGTGAAGTTTTGGATCACGCTGAATGAGCCCTTT GTCATTGCTTACCAGGGCTATGGCTACGGAACAGCAGCTCCAGGAGTCTCCAATAGGCCTGGCACTGCCC CCTACATTGTTGGCCACAATCTAATAAAGGCTCATGCTGAGGCCTGGCATCTGTACAACGATGTGTACCG CGCCAGTCAAGGTGGCGTGATTTCCATCACCATCAGCAGTGACTGGGCTGAACCCAGAGATCCCTCTAAC CAGGAGGATGTGGAGGCAGCCAGGAGATATGTTCAGTTCATGGGAGGCTGGTTTGCACATCCTATTTTCA AGAATGGAGATTACAATGAGGTGATGAAGACGCGGATCCGTGACAGGAGCTTGGCTGCAGGCCTCAACAA GTCTCGGCTGCCAGAATTTACAGAGAGTGAGAAGAGGAGGATCAACGGCACCTATGACTTTTTTGGGTTC AATCACTACACCACTGTCCTCGCCTACAACCTCAACTATGCCACTGCCATCTCTTCTTTTGATGCAGACA GAGGAGTTGCTTCCATCGCAGATCGCTCGTGGCCAGACTCTGGCTCCTTCTGGCTGAAGATGACGCCTTT TGGCTTCAGGAGGATCCTGAACTGGTTAAAGGAGGAATACAATGACCCTCCAATTTATGTCACAGAGAAT GGAGTGTCCCAGCGGGAAGAAACAGACCTCAATGACACTGCAAGGATCTACTACCTTCGGACTTACATCA ATGAGGCCCTCAAAGCTGTGCAGGACAAGGTGGACCTTCGAGGATACACAGTTTGGAGTGCGATGGACAA TTTTGAGTGGGCCACAGGCTTTTCAGAGAGATTTGGTCTGCATTTTGTGAACTACAGTGACCCTTCTCTG CCAAGGATCCCCAAAGCATCAGCGAAGTTCTACGCCTCTGTGGTCCGATGCAATGGCTTCCCTGACCCCG CTACAGGGCCTCACGCTTGTCTCCACCAGCCAGATGCTGGACCCACCATCAGCCCCGTGAGACAGGAGGA GGTGCAGTTCCTGGGGCTAATGCTCGGCACCACAGAAGCACAGACAGCTTTGTACGTTCTCTTTTCTCTT GTGCTTCTTGGAGTCTGTGGCTTGGCATTTCTGTCATACAAGTACTGCAAGCGCTCTAAGCAAGGGAAAA CACAACGAAGCCAACAGGAATTGAGCCCGGTGTCTTCATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212751 representing NM_002299
Red=Cloning site Green=Tags(s) MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPT FLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPAST LRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESY AFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFN LKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHET TDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTT EGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIE PMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGI SDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGW FAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCI PSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSL RVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTS IIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWG VSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISW SRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCF QTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVI SLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFT EEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWI KEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTV KFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADG KGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGL NYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPF VIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSN QEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGF NHYTTVLAYNLNYATAISSFDADRGVASIADRSWPDSGSFWLKMTPFGFRRILNWLKEEYNDPPIYVTEN GVSQREETDLNDTARIYYLRTYINEALKAVQDKVDLRGYTVWSAMDNFEWATGFSERFGLHFVNYSDPSL PRIPKASAKFYASVVRCNGFPDPATGPHACLHQPDAGPTISPVRQEEVQFLGLMLGTTEAQTALYVLFSL VLLGVCGLAFLSYKYCKRSKQGKTQRSQQELSPVSSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002299 |
| ORF Size | 5781 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002299.4 |
| RefSeq Size | 6274 bp |
| RefSeq ORF | 5784 bp |
| Locus ID | 3938 |
| UniProt ID | P09848 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Galactose metabolism |
| MW | 218.59 kDa |
| Gene Summary | The protein encoded by this gene belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are associated with congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212751L1 | Lenti ORF clone of Human lactase (LCT), Myc-DDK-tagged |
CNY 15,352.00 |
|
| RG212751 | LCT (tGFP-tagged) - Human lactase (LCT) |
CNY 15,200.00 |
|
| SC303171 | LCT (untagged)-Human lactase (LCT) |
CNY 37,050.00 |
