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APOL3 (NM_145641) Human Tagged ORF Clone
CAT＃: RC212625
APOL3 (Myc-DDK-tagged)-Human apolipoprotein L, 3 (APOL3), transcript variant beta/a
ORF Plasmid: tGFP
"NM_145641" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | apoL-III; APOLIII; CG12_1; CG121 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212625 representing NM_145641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTTGCTGGTCTTGTTTTGGCACCATTTACAGCAGGGACGAGTCTGGCCCTTACTGCAGCTGGGG TAGGGCTGGGAGCAGCGTCTGCTGTGACTGGGATCACCACCAGCATCGTGGAGCACTCATATACATCATC AGCAGAAGCTGAAGCCAGCAGGCTGACTGCAACCAGCATTGACCGATTGAAGGTATTTAAGGAAGTTATG CGTGACATCACACCCAACTTACTTTCCCTTCTTAATAATTATTACGAAGCCACACAAACCATTGGGAGTG AAATCCGTGCCATCAGGCAAGCCAGAGCCAGGGCCCGACTCCCTGTGACCACCTGGCGAATCTCAGCTGG AAGTGGTGGTCAAGCAGAGAGAACGATTGCAGGCACCACCCGGGCAGTGAGCAGAGGAGCCCGGATCCTG AGTGCGACCACTTCAGGCATCTTCCTTGCACTGGATGTGGTCAACCTTGTATACGAGTCAAAGCACTTGC ATGAGGGGGCAAAGTCTGCATCTGCTGAGGAGCTGAGGCGGCAGGCTCAGGAGCTGGAGGAGAATCTAAT GGAGCTCACTCAGATCTATCAGCGTCTGAATCCATGCCATACCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212625 representing NM_145641
Red=Cloning site Green=Tags(s) MSLAGLVLAPFTAGTSLALTAAGVGLGAASAVTGITTSIVEHSYTSSAEAEASRLTATSIDRLKVFKEVM RDITPNLLSLLNNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRGARIL SATTSGIFLALDVVNLVYESKHLHEGAKSASAEELRRQAQELEENLMELTQIYQRLNPCHTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145641 |
| ORF Size | 606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145641.2 |
| RefSeq Size | 3627 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 80833 |
| UniProt ID | O95236 |
| MW | 21.4 kDa |
| Gene Summary | This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212625L1 | Lenti ORF clone of Human apolipoprotein L, 3 (APOL3), transcript variant beta/a, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC212625L2 | Lenti ORF clone of Human apolipoprotein L, 3 (APOL3), transcript variant beta/a, mGFP tagged |
CNY 5,890.00 |
|
| RC212625L3 | Lenti ORF clone of Human apolipoprotein L, 3 (APOL3), transcript variant beta/a, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212625L4 | Lenti ORF clone of Human apolipoprotein L, 3 (APOL3), transcript variant beta/a, mGFP tagged |
CNY 5,890.00 |
|
| RG212625 | APOL3 (tGFP-tagged) - Human apolipoprotein L, 3 (APOL3), transcript variant beta/a |
CNY 4,000.00 |
|
| SC110655 | APOL3 (untagged)-Human apolipoprotein L, 3 (APOL3), transcript variant beta/a |
CNY 2,400.00 |
