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LLGL2 (NM_004524) Human Tagged ORF Clone
CAT＃: RC212604
- TrueORF®
LLGL2 (Myc-DDK-tagged)-Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 1
ORF Plasmid: tGFP
"NM_004524" in other vectors (4)
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CNY 10,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HGL; Hugl-2; LGL2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212604 representing NM_004524
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTTCCTGAGGCCAGGGCATGACCCTGTGCGGGAGAGGCTCAAGCGGGACCTGTTCCAGTTTA ACAAGACGGTGGAGCATGGCTTCCCGCACCAGCCCAGCGCCCTCGGCTACAGCCCGTCCCTGCGCATCCT GGCCATCGGCACCCGTTCTGGAGCCATCAAGCTCTACGGAGCCCCAGGCGTGGAGTTCATGGGGCTGCAC CAGGAGAACAACGCTGTGACGCAGATCCACCTCCTGCCCGGCCAGTGCCAGCTGGTCACCCTGCTGGATG ACAACAGCCTGCACCTTTGGAGCCTGAAGGTCAAGGGCGGGGCATCGGAGCTGCAGGAGGATGAGAGCTT CACACTGCGTGGACCCCCAGGGGCTGCCCCCAGTGCCACACAGATCACCGTGGTCCTGCCACATTCCTCC TGCGAGCTGCTCTACCTGGGCACCGAGAGTGGCAACGTGTTTGTGGTGCAGCTGCCAGCTTTTCGTGCGC TGGAGGACCGGACCATCAGCTCGGACGCGGTGCTGCAGCGGTTGCCAGAGGAGGCCCGCCACCGGCGTGT GTTCGAGATGGTGGAGGCACTGCAGGAGCACCCTCGAGACCCCAACCAGATCCTGATCGGCTACAGCCGA GGCCTCGTTGTCATCTGGGACCTACAGGGCAGCCGCGTGCTCTACCACTTCCTCAGCAGCCAGCAACTGG AGAACATCTGGTGGCAGCGGGACGGCCGCCTGCTCGTCAGCTGTCACTCTGACGGCAGCTACTGCCAGTG GCCCGTGTCCAGCGAAGCCCAGCAACCAGAGCCCCTCCGCAGCCTCGTGCCTTACGGTCCCTTTCCTTGC AAAGCGATTACCAGAATCCTCTGGCTGACCACTAGGCAGGGGTTGCCCTTCACCATCTTCCAGGGTGGCA TGCCACGGGCCAGCTACGGGGACCGCCACTGCATCTCAGTGATCCACGATGGCCAGCAGACGGCCTTCGA CTTCACCTCCCGTGTCATCGGCTTCACTGTCCTCACAGAGGCAGACCCTGCAGCCACCTTTGACGACCCC TATGCCCTGGTGGTGCTGGCTGAGGAGGAGCTGGTGGTGATTGACCTGCAGACAGCAGGCTGGCCACCGG TCCAGCTGCCCTACCTGGCTTCTCTGCACTGTTCCGCCATCACCTGCTCTCACCACGTCTCCAACATCCC GCTGAAGCTGTGGGAGCGGATCATTGCCGCCGGCAGCCGGCAGAACGCACACTTCTCCACCATGGAGTGG CCAATTGATGGTGGCACCAGCCTGACCCCAGCCCCACCCCAGAGGGACCTGCTGCTCACAGGGCACGAGG ACGGCACGGTGCGGTTCTGGGATGCCTCGGGTGTCTGCCTGCGGCTGCTCTACAAACTCAGCACTGTGCG CGTGTTCCTCACCGACACGGACCCCAACGAGAACTTCAGTGCCCAGGGCGAGGACGAGTGGCCCCCACTC CGCAAGGTGGGCTCCTTTGACCCCTACAGTGATGACCCCCGGCTGGGCATCCAGAAGATCTTCCTCTGCA AGTACAGCGGCTACCTGGCTGTGGCAGGCACGGCAGGGCAGGTGCTGGTACTGGAACTGAATGACGAGGC AGCGGAGCAGGCTGTGGAGCAGGTGGAGGCCGACCTGCTGCAGGACCAAGAGGGCTACCGCTGGAAGGGG CACGAGCGCCTGGCAGCCCGCTCAGGGCCCGTGCGCTTTGAGCCTGGCTTTCAGCCCTTCGTGTTGGTGC AGTGTCAGCCCCCGGCTGTGGTCACCTCCTTGGCCCTGCACTCTGAGTGGCGGCTCGTGGCCTTCGGCAC CAGCCATGGCTTTGGCCTCTTTGACCACCAGCAGCGGCGGCAGGTCTTTGTTAAGTGCACACTGCACCCC AGTGACCAGCTGGCCTTGGAGGGCCCACTCTCCCGCGTCAAGTCCCTCAAGAAGTCCTTGCGTCAGTCAT TCCGCCGGATGCGTCGGAGCCGGGTGTCCAGCCGGAAGCGGCACCCGGCTGGCCCCCCAGGAGAGGCACA GGAGGGGAGTGCCAAGGCTGAGCGGCCAGGCCTCCAGAACATGGAGCTGGCGCCTGTGCAGCGCAAGATC GAGGCTCGCTCGGCAGAGGACTCCTTCACAGGCTTCGTCCGGACCCTGTACTTTGCTGACACCTACCTGA AGGACAGCTCCCGGCACTGCCCCTCGCTGTGGGCTGGCACCAATGGGGGCACCATCTATGCCTTCTCCCT GCGTGTGCCTCCCGCCGAGCGGAGAATGGATGAGCCTGTGCGGGCAGAGCAGGCCAAGGAGATCCAGCTG ATGCACCGGGCGCCGGTGGTGGGCATCCTGGTGCTCGACGGACACAGCGTACCCCTTCCCGAGCCCCTCG AAGTGGCCCATGATCTGTCGAAGAGCCCTGACATGCAGGGAAGCCACCAGCTGCTCGTCGTATCAGAGGA GCAGTTCAAGGTGTTCACGCTGCCCAAGGTGAGTGCCAAGCTGAAGTTGAAGCTGACGGCCCTGGAGGGC TCAAGAGTGCGGCGGGTCAGCGTGGCCCACTTCGGCAGTCGTCGAGCCGAGGACTACGGGGAGCACCACC TGGCAGTCCTTACCAACCTGGGCGACATCCAGGTGGTCTCGCTGCCCCTGCTCAAGCCCCAGGTGCGCTA CAGCTGCATCCGCCGGGAGGACGTCAGTGGCATCGCCTCCTGCGTCTTCACCAAATATGGCCAAGGCTTC TACCTGATCTCACCCTCGGAGTTTGAGCGCTTCTCTCTCTCCACCAAGTGGCTGGTGGAGCCCCGGTGTC TGGTGGATTCAGCAGAAACCAAGAACCACCGCCCTGGTAACGGTGCGGGCCCCAAGAAGGCCCCGAGCCG AGCCAGGAACTCAGGGACTCAGAGTGATGGCGAGGAGAAGCAGCCCGGCCTGGTGATGGAGCGCGCTCTG CTCAGTGATGAGAGAGCGGCAACTGGCGTTCACATCGAGCCGCCGTGGGGTGCAGCCTCAGCAATGGCGG AGCAGAGTGAGTGGCTGAGCGTCCAGGCTGCGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212604 representing NM_004524
Red=Cloning site Green=Tags(s) MRRFLRPGHDPVRERLKRDLFQFNKTVEHGFPHQPSALGYSPSLRILAIGTRSGAIKLYGAPGVEFMGLH QENNAVTQIHLLPGQCQLVTLLDDNSLHLWSLKVKGGASELQEDESFTLRGPPGAAPSATQITVVLPHSS CELLYLGTESGNVFVVQLPAFRALEDRTISSDAVLQRLPEEARHRRVFEMVEALQEHPRDPNQILIGYSR GLVVIWDLQGSRVLYHFLSSQQLENIWWQRDGRLLVSCHSDGSYCQWPVSSEAQQPEPLRSLVPYGPFPC KAITRILWLTTRQGLPFTIFQGGMPRASYGDRHCISVIHDGQQTAFDFTSRVIGFTVLTEADPAATFDDP YALVVLAEEELVVIDLQTAGWPPVQLPYLASLHCSAITCSHHVSNIPLKLWERIIAAGSRQNAHFSTMEW PIDGGTSLTPAPPQRDLLLTGHEDGTVRFWDASGVCLRLLYKLSTVRVFLTDTDPNENFSAQGEDEWPPL RKVGSFDPYSDDPRLGIQKIFLCKYSGYLAVAGTAGQVLVLELNDEAAEQAVEQVEADLLQDQEGYRWKG HERLAARSGPVRFEPGFQPFVLVQCQPPAVVTSLALHSEWRLVAFGTSHGFGLFDHQQRRQVFVKCTLHP SDQLALEGPLSRVKSLKKSLRQSFRRMRRSRVSSRKRHPAGPPGEAQEGSAKAERPGLQNMELAPVQRKI EARSAEDSFTGFVRTLYFADTYLKDSSRHCPSLWAGTNGGTIYAFSLRVPPAERRMDEPVRAEQAKEIQL MHRAPVVGILVLDGHSVPLPEPLEVAHDLSKSPDMQGSHQLLVVSEEQFKVFTLPKVSAKLKLKLTALEG SRVRRVSVAHFGSRRAEDYGEHHLAVLTNLGDIQVVSLPLLKPQVRYSCIRREDVSGIASCVFTKYGQGF YLISPSEFERFSLSTKWLVEPRCLVDSAETKNHRPGNGAGPKKAPSRARNSGTQSDGEEKQPGLVMERAL LSDERAATGVHIEPPWGAASAMAEQSEWLSVQAAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004524 |
| ORF Size | 3045 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004524.3 |
| RefSeq Size | 3547 bp |
| RefSeq ORF | 3048 bp |
| Locus ID | 3993 |
| UniProt ID | Q6P1M3 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| Protein Pathways | Tight junction |
| MW | 113.4 kDa |
| Gene Summary | The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212604L3 | Lenti-ORF clone of LLGL2 (Myc-DDK-tagged)-Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 1 |
CNY 12,070.00 |
|
| RC212604L4 | Lenti-ORF clone of LLGL2 (mGFP-tagged)-Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 1 |
CNY 12,070.00 |
|
| RG212604 | LLGL2 (tGFP-tagged) - Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 1 |
CNY 11,210.00 |
|
| SC310006 | LLGL2 (untagged)-Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 1 |
CNY 19,570.00 |
